Bone Regeneration Induced by Bone Porcine Block with Bone Marrow Stromal Stem Cells in a Minipig Model of Mandibular “Critical Size” Defect
Introduction. Adding stem cells to biodegradable scaffolds to enhance bone regeneration is a valuable option. Different kinds of stem cells with osteoblastic activity were tested, such as bone marrow stromal stem cells (BMSSCs). Aim. To assess a correct protocol for osteogenic stem cell differentiat...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/9082869 |
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| author | Antonio Scarano Vito Crincoli Adriana Di Benedetto Valerio Cozzolino Felice Lorusso Michele Podaliri Vulpiani Maria Grano Zamira Kalemaj Giorgio Mori Felice Roberto Grassi |
| author_facet | Antonio Scarano Vito Crincoli Adriana Di Benedetto Valerio Cozzolino Felice Lorusso Michele Podaliri Vulpiani Maria Grano Zamira Kalemaj Giorgio Mori Felice Roberto Grassi |
| author_sort | Antonio Scarano |
| collection | DOAJ |
| description | Introduction. Adding stem cells to biodegradable scaffolds to enhance bone regeneration is a valuable option. Different kinds of stem cells with osteoblastic activity were tested, such as bone marrow stromal stem cells (BMSSCs). Aim. To assess a correct protocol for osteogenic stem cell differentiation, so BMSSCs were seeded on a bone porcine block (BPB). Materials and Methods. Bone marrow from six minipigs was extracted from tibiae and humeri and treated to isolate BMSSCs. After seeding on BPB, critical-size defects were created on each mandible of the minipigs and implanted with BPB and BPB/BMSSCs. After three months, histomorphometric analysis was performed. Results. Histomorphometric analysis provided percentages of the three groups. Tissues present in control defects were 23 ± 2% lamellar bone, 28 ± 1% woven bone, and 56 ± 4% marrow spaces; in BPB defects were 20 ± 5% BPB, 32 ± 2% lamellar bone, 24 ± 1% woven bone, and 28 ± 2% marrow spaces; in BPB/BMSSCs defects were 17 ± 4% BPB/BMSSCs, 42 ± 2% lamellar bone, 12 ± 1% woven bone, and 22 ± 3% marrow spaces. Conclusion. BPB used as a scaffold to induce bone regeneration may benefit from the addition of BDPSCs in the tissue-engineered constructs. |
| format | Article |
| id | doaj-art-d58d507c62aa403b84998e27183b5bb6 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-d58d507c62aa403b84998e27183b5bb62025-02-03T05:58:50ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/90828699082869Bone Regeneration Induced by Bone Porcine Block with Bone Marrow Stromal Stem Cells in a Minipig Model of Mandibular “Critical Size” DefectAntonio Scarano0Vito Crincoli1Adriana Di Benedetto2Valerio Cozzolino3Felice Lorusso4Michele Podaliri Vulpiani5Maria Grano6Zamira Kalemaj7Giorgio Mori8Felice Roberto Grassi9Department of Medical, Oral and Biotechnological Sciences and CeSI-Met, University of Chieti-Pescara, Chieti, ItalyInterdisciplinary Department of Medicine, University of Bari "A. Moro", Bari, ItalyDepartment of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, ItalyDepartment of Medical, Oral and Biotechnological Sciences and CeSI-Met, University of Chieti-Pescara, Chieti, ItalyDepartment of Medical, Oral and Biotechnological Sciences and CeSI-Met, University of Chieti-Pescara, Chieti, ItalyDepartment of Animals Sperimentation, Istituto Zooprofilattico Sperimentale dell'Abruzzo e del Molise "G. Caporale", Teramo, ItalyDepartment of Emergency and Organ Transplantation, University of Bari "A. Moro", Bari, ItalyDepartment of Surgical Sciences, Dental School, University of Turin, Torino, ItalyDepartment of Clinical and Experimental Medicine, Medical School, University of Foggia, Foggia, ItalyDepartment of Basic and Medical Sciences, Neurosciences and Sense Organs, University of Bari, Bari, ItalyIntroduction. Adding stem cells to biodegradable scaffolds to enhance bone regeneration is a valuable option. Different kinds of stem cells with osteoblastic activity were tested, such as bone marrow stromal stem cells (BMSSCs). Aim. To assess a correct protocol for osteogenic stem cell differentiation, so BMSSCs were seeded on a bone porcine block (BPB). Materials and Methods. Bone marrow from six minipigs was extracted from tibiae and humeri and treated to isolate BMSSCs. After seeding on BPB, critical-size defects were created on each mandible of the minipigs and implanted with BPB and BPB/BMSSCs. After three months, histomorphometric analysis was performed. Results. Histomorphometric analysis provided percentages of the three groups. Tissues present in control defects were 23 ± 2% lamellar bone, 28 ± 1% woven bone, and 56 ± 4% marrow spaces; in BPB defects were 20 ± 5% BPB, 32 ± 2% lamellar bone, 24 ± 1% woven bone, and 28 ± 2% marrow spaces; in BPB/BMSSCs defects were 17 ± 4% BPB/BMSSCs, 42 ± 2% lamellar bone, 12 ± 1% woven bone, and 22 ± 3% marrow spaces. Conclusion. BPB used as a scaffold to induce bone regeneration may benefit from the addition of BDPSCs in the tissue-engineered constructs.http://dx.doi.org/10.1155/2017/9082869 |
| spellingShingle | Antonio Scarano Vito Crincoli Adriana Di Benedetto Valerio Cozzolino Felice Lorusso Michele Podaliri Vulpiani Maria Grano Zamira Kalemaj Giorgio Mori Felice Roberto Grassi Bone Regeneration Induced by Bone Porcine Block with Bone Marrow Stromal Stem Cells in a Minipig Model of Mandibular “Critical Size” Defect Stem Cells International |
| title | Bone Regeneration Induced by Bone Porcine Block with Bone Marrow Stromal Stem Cells in a Minipig Model of Mandibular “Critical Size” Defect |
| title_full | Bone Regeneration Induced by Bone Porcine Block with Bone Marrow Stromal Stem Cells in a Minipig Model of Mandibular “Critical Size” Defect |
| title_fullStr | Bone Regeneration Induced by Bone Porcine Block with Bone Marrow Stromal Stem Cells in a Minipig Model of Mandibular “Critical Size” Defect |
| title_full_unstemmed | Bone Regeneration Induced by Bone Porcine Block with Bone Marrow Stromal Stem Cells in a Minipig Model of Mandibular “Critical Size” Defect |
| title_short | Bone Regeneration Induced by Bone Porcine Block with Bone Marrow Stromal Stem Cells in a Minipig Model of Mandibular “Critical Size” Defect |
| title_sort | bone regeneration induced by bone porcine block with bone marrow stromal stem cells in a minipig model of mandibular critical size defect |
| url | http://dx.doi.org/10.1155/2017/9082869 |
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