BV/ODV-E26 is a conserved baculoviral inhibitory factor for optimizing viral virulence in lepidopteran hosts
Summary: Alphabaculoviruses induce abnormal behavior in lepidopteran larval hosts. A baculoviral gene, bv/odv-e26, is crucial for behavioral manipulation in Bombyx mori larvae by Bombyx mori nucleopolyhedrovirus (BmNPV). However, how bv/odv-e26 fulfills its role in this phenotype remains largely unk...
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Elsevier
2025-02-01
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| Series: | iScience |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422402950X |
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| author | Hiroyuki Hikida Ryuhei Kokusho Susumu Katsuma |
| author_facet | Hiroyuki Hikida Ryuhei Kokusho Susumu Katsuma |
| author_sort | Hiroyuki Hikida |
| collection | DOAJ |
| description | Summary: Alphabaculoviruses induce abnormal behavior in lepidopteran larval hosts. A baculoviral gene, bv/odv-e26, is crucial for behavioral manipulation in Bombyx mori larvae by Bombyx mori nucleopolyhedrovirus (BmNPV). However, how bv/odv-e26 fulfills its role in this phenotype remains largely unknown. In this study, we found that the overexpression of BmNPV bv/odv-e26 delayed viral infection in cultured cells and decreased pathogenicity in B. mori larvae. We also discovered that homologs of bv/odv-e26 are conserved more widely in alphabaculoviruses than previously thought. The inhibitory activity was demonstrated in bv/odv-e26 homologs of phylogenetically close and distant baculoviruses, indicating conserved inhibitory function among alphabaculoviruses. Furthermore, locomotory analyses revealed that bv/odv-e26 increased larval locomotory activity but had little effect on the timing of abnormal behavior initiation. Collectively, our findings demonstrate that bv/odv-e26 is a baculoviral inhibitory factor that is widely conserved in the genus alphabaculovirus and may reduce viral virulence for successful host behavioral manipulation. |
| format | Article |
| id | doaj-art-d5169b4b04bc4ef098888d40605181a2 |
| institution | Kabale University |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-d5169b4b04bc4ef098888d40605181a22025-01-19T06:26:29ZengElsevieriScience2589-00422025-02-01282111723BV/ODV-E26 is a conserved baculoviral inhibitory factor for optimizing viral virulence in lepidopteran hostsHiroyuki Hikida0Ryuhei Kokusho1Susumu Katsuma2Institute for Chemical Research, Kyoto University, Gokasho, Uji, Kyoto 611-0011, Japan; Department of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Corresponding authorDepartment of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, JapanDepartment of Agricultural and Environmental Biology, Graduate School of Agricultural and Life Sciences, The University of Tokyo, 1-1-1, Yayoi, Bunkyo-ku, Tokyo 113-8657, Japan; Corresponding authorSummary: Alphabaculoviruses induce abnormal behavior in lepidopteran larval hosts. A baculoviral gene, bv/odv-e26, is crucial for behavioral manipulation in Bombyx mori larvae by Bombyx mori nucleopolyhedrovirus (BmNPV). However, how bv/odv-e26 fulfills its role in this phenotype remains largely unknown. In this study, we found that the overexpression of BmNPV bv/odv-e26 delayed viral infection in cultured cells and decreased pathogenicity in B. mori larvae. We also discovered that homologs of bv/odv-e26 are conserved more widely in alphabaculoviruses than previously thought. The inhibitory activity was demonstrated in bv/odv-e26 homologs of phylogenetically close and distant baculoviruses, indicating conserved inhibitory function among alphabaculoviruses. Furthermore, locomotory analyses revealed that bv/odv-e26 increased larval locomotory activity but had little effect on the timing of abnormal behavior initiation. Collectively, our findings demonstrate that bv/odv-e26 is a baculoviral inhibitory factor that is widely conserved in the genus alphabaculovirus and may reduce viral virulence for successful host behavioral manipulation.http://www.sciencedirect.com/science/article/pii/S258900422402950XTranscriptomicsVirology |
| spellingShingle | Hiroyuki Hikida Ryuhei Kokusho Susumu Katsuma BV/ODV-E26 is a conserved baculoviral inhibitory factor for optimizing viral virulence in lepidopteran hosts iScience Transcriptomics Virology |
| title | BV/ODV-E26 is a conserved baculoviral inhibitory factor for optimizing viral virulence in lepidopteran hosts |
| title_full | BV/ODV-E26 is a conserved baculoviral inhibitory factor for optimizing viral virulence in lepidopteran hosts |
| title_fullStr | BV/ODV-E26 is a conserved baculoviral inhibitory factor for optimizing viral virulence in lepidopteran hosts |
| title_full_unstemmed | BV/ODV-E26 is a conserved baculoviral inhibitory factor for optimizing viral virulence in lepidopteran hosts |
| title_short | BV/ODV-E26 is a conserved baculoviral inhibitory factor for optimizing viral virulence in lepidopteran hosts |
| title_sort | bv odv e26 is a conserved baculoviral inhibitory factor for optimizing viral virulence in lepidopteran hosts |
| topic | Transcriptomics Virology |
| url | http://www.sciencedirect.com/science/article/pii/S258900422402950X |
| work_keys_str_mv | AT hiroyukihikida bvodve26isaconservedbaculoviralinhibitoryfactorforoptimizingviralvirulenceinlepidopteranhosts AT ryuheikokusho bvodve26isaconservedbaculoviralinhibitoryfactorforoptimizingviralvirulenceinlepidopteranhosts AT susumukatsuma bvodve26isaconservedbaculoviralinhibitoryfactorforoptimizingviralvirulenceinlepidopteranhosts |