Molecular detection of Trypanosoma minasense in captive black-faced black spider monkeys Ateles chamek (mammalia; primate)
The present study aimed to assess Trypanosoma spp. infection in 17 captive black-faced black spider monkeys (Ateles chamek), living at the Zoological Garden of Curitiba, Paraná, Brazil. Blood samples (3 mL) were collected by femoral puncture by certified veterinarians. A total of 5 μL of extracted b...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Veterinary Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fvets.2024.1527193/full |
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| author | Louise Bach Kmetiuk Louise Bach Kmetiuk Ana Silvia Miranda Passerino Marcelo Bonat Vívien Midori Morikawa Alice Pereira Berbigier André Luiz Rodrigues Roque Fabiano Borges Figueiredo Alexander Welker Biondo |
| author_facet | Louise Bach Kmetiuk Louise Bach Kmetiuk Ana Silvia Miranda Passerino Marcelo Bonat Vívien Midori Morikawa Alice Pereira Berbigier André Luiz Rodrigues Roque Fabiano Borges Figueiredo Alexander Welker Biondo |
| author_sort | Louise Bach Kmetiuk |
| collection | DOAJ |
| description | The present study aimed to assess Trypanosoma spp. infection in 17 captive black-faced black spider monkeys (Ateles chamek), living at the Zoological Garden of Curitiba, Paraná, Brazil. Blood samples (3 mL) were collected by femoral puncture by certified veterinarians. A total of 5 μL of extracted blood DNA was submitted to Nested-PCR 18S rDNA. In overall, 4/17 (23.5%) spider monkeys were positive to Trypanosoma spp. by PCR. Sequencing results for 18S rDNA revealed Trypanosoma minasense infection. This is the first report of T. minasense in black-faced black spider monkey worldwide and the first assessment of Trypanosoma species in NHP of southern Brazil. |
| format | Article |
| id | doaj-art-d5132a8868d74799a20cacf3db5225de |
| institution | Kabale University |
| issn | 2297-1769 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Veterinary Science |
| spelling | doaj-art-d5132a8868d74799a20cacf3db5225de2025-01-23T14:24:45ZengFrontiers Media S.A.Frontiers in Veterinary Science2297-17692025-01-011110.3389/fvets.2024.15271931527193Molecular detection of Trypanosoma minasense in captive black-faced black spider monkeys Ateles chamek (mammalia; primate)Louise Bach Kmetiuk0Louise Bach Kmetiuk1Ana Silvia Miranda Passerino2Marcelo Bonat3Vívien Midori Morikawa4Alice Pereira Berbigier5André Luiz Rodrigues Roque6Fabiano Borges Figueiredo7Alexander Welker Biondo8Graduate Program in Biosciences and Biotechnology, Instituto Carlos Chagas, Fundação Oswaldo Cruz, Curitiba, Paraná, BrazilCuritiba City Secretary of Health, Curitiba, Paraná, BrazilCuritiba City Secretary of Environment, Curitiba, Paraná, BrazilCuritiba City Secretary of Environment, Curitiba, Paraná, BrazilCuritiba City Secretary of Environment, Curitiba, Paraná, BrazilLaboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, BrazilLaboratory of Trypanosomatid Biology, Oswaldo Cruz Institute, Oswaldo Cruz Foundation, Rio de Janeiro, Rio de Janeiro, BrazilGraduate Program in Biosciences and Biotechnology, Instituto Carlos Chagas, Fundação Oswaldo Cruz, Curitiba, Paraná, BrazilDepartment of Veterinary Medicine, Federal University of Paraná, Curitiba, Paraná, BrazilThe present study aimed to assess Trypanosoma spp. infection in 17 captive black-faced black spider monkeys (Ateles chamek), living at the Zoological Garden of Curitiba, Paraná, Brazil. Blood samples (3 mL) were collected by femoral puncture by certified veterinarians. A total of 5 μL of extracted blood DNA was submitted to Nested-PCR 18S rDNA. In overall, 4/17 (23.5%) spider monkeys were positive to Trypanosoma spp. by PCR. Sequencing results for 18S rDNA revealed Trypanosoma minasense infection. This is the first report of T. minasense in black-faced black spider monkey worldwide and the first assessment of Trypanosoma species in NHP of southern Brazil.https://www.frontiersin.org/articles/10.3389/fvets.2024.1527193/fullcaptive primatesblood parasitesmolecular analysisnon-human primatesTrypanosoma spp. |
| spellingShingle | Louise Bach Kmetiuk Louise Bach Kmetiuk Ana Silvia Miranda Passerino Marcelo Bonat Vívien Midori Morikawa Alice Pereira Berbigier André Luiz Rodrigues Roque Fabiano Borges Figueiredo Alexander Welker Biondo Molecular detection of Trypanosoma minasense in captive black-faced black spider monkeys Ateles chamek (mammalia; primate) Frontiers in Veterinary Science captive primates blood parasites molecular analysis non-human primates Trypanosoma spp. |
| title | Molecular detection of Trypanosoma minasense in captive black-faced black spider monkeys Ateles chamek (mammalia; primate) |
| title_full | Molecular detection of Trypanosoma minasense in captive black-faced black spider monkeys Ateles chamek (mammalia; primate) |
| title_fullStr | Molecular detection of Trypanosoma minasense in captive black-faced black spider monkeys Ateles chamek (mammalia; primate) |
| title_full_unstemmed | Molecular detection of Trypanosoma minasense in captive black-faced black spider monkeys Ateles chamek (mammalia; primate) |
| title_short | Molecular detection of Trypanosoma minasense in captive black-faced black spider monkeys Ateles chamek (mammalia; primate) |
| title_sort | molecular detection of trypanosoma minasense in captive black faced black spider monkeys ateles chamek mammalia primate |
| topic | captive primates blood parasites molecular analysis non-human primates Trypanosoma spp. |
| url | https://www.frontiersin.org/articles/10.3389/fvets.2024.1527193/full |
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