Role of mast cell in locally advanced rectal cancer treated with neoadjuvant chemoradiotherapy
Abstract The pro-tumor effects of mast cell (MC) in the tumor microenvironment (TME) are becoming increasingly clear. Recently, MC were shown to contribute to tumor malignancy by supporting the migration of tumor-associated macrophages (TAMs), suggesting a relationship with tumor immunity. In the cu...
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BMC
2025-01-01
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| Series: | BMC Cancer |
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| Online Access: | https://doi.org/10.1186/s12885-025-13458-9 |
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| author | Masaaki Nishi Shoko Yamashita Chie Takasu Yuma Wada Kozo Yoshikawa Takuya Tokunaga Toshihiro Nakao Hideya Kashihara Toshiaki Yoshimoto Mitsuo Shimada |
| author_facet | Masaaki Nishi Shoko Yamashita Chie Takasu Yuma Wada Kozo Yoshikawa Takuya Tokunaga Toshihiro Nakao Hideya Kashihara Toshiaki Yoshimoto Mitsuo Shimada |
| author_sort | Masaaki Nishi |
| collection | DOAJ |
| description | Abstract The pro-tumor effects of mast cell (MC) in the tumor microenvironment (TME) are becoming increasingly clear. Recently, MC were shown to contribute to tumor malignancy by supporting the migration of tumor-associated macrophages (TAMs), suggesting a relationship with tumor immunity. In the current study, we aimed to examine the correlation between MC infiltration and neoadjuvant chemoradiotherapy (nCRT) response for locally advanced rectal cancer (LARC). Ninety-five LARC patients who recieved nCRT were enrolled in this study. Protein levels of the MC marker tryptase and TAM marker CD206 were evaluated with immunohistochemistry (IHC). The correlation between MC infiltration and prognostic factors was evaluated. The effects of MCs on the malignant potential were examined using in vitro proliferation and invasion assays with a colorectal cancer (CRC) cell line (HCT-116). Following nCRT, 31.6% of resected LARC patient specimens were positive for MC infiltration by tryptase IHC analysis. MC infiltration was significantly correlated with nCRT response. The 5-year disease-free survival (DFS) rate was significantly lower in the MC-positive group compared with the MC-negative group (52.3% vs. 76.8%). Univariate and multivariate analyses revealed that MC infiltration was the independent prognostic indicator for DFS. MC infiltration was significantly correlated with CD206 expression, and therefore TAMs. In vitro experiments suggested that tumor activated mast cells could promote CRC cell malignant behavior via production of macrophage inhibitory factor. MC infiltration in LARC patients was positively correlated with TAM infiltration and resistance to nCRT, and was also an independent poor prognostic indicator. |
| format | Article |
| id | doaj-art-d504556fc42b4fa0a2f6aa3aa3d0b9fb |
| institution | Kabale University |
| issn | 1471-2407 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Cancer |
| spelling | doaj-art-d504556fc42b4fa0a2f6aa3aa3d0b9fb2025-01-19T12:26:43ZengBMCBMC Cancer1471-24072025-01-0125111010.1186/s12885-025-13458-9Role of mast cell in locally advanced rectal cancer treated with neoadjuvant chemoradiotherapyMasaaki Nishi0Shoko Yamashita1Chie Takasu2Yuma Wada3Kozo Yoshikawa4Takuya Tokunaga5Toshihiro Nakao6Hideya Kashihara7Toshiaki Yoshimoto8Mitsuo Shimada9Department of Surgery, Tokushima UniversityDepartment of Surgery, Tokushima UniversityDepartment of Surgery, Tokushima UniversityDepartment of Surgery, Tokushima UniversityDepartment of Surgery, Tokushima UniversityDepartment of Surgery, Tokushima UniversityDepartment of Surgery, Tokushima UniversityDepartment of Surgery, Tokushima UniversityDepartment of Surgery, Tokushima UniversityDepartment of Surgery, Tokushima UniversityAbstract The pro-tumor effects of mast cell (MC) in the tumor microenvironment (TME) are becoming increasingly clear. Recently, MC were shown to contribute to tumor malignancy by supporting the migration of tumor-associated macrophages (TAMs), suggesting a relationship with tumor immunity. In the current study, we aimed to examine the correlation between MC infiltration and neoadjuvant chemoradiotherapy (nCRT) response for locally advanced rectal cancer (LARC). Ninety-five LARC patients who recieved nCRT were enrolled in this study. Protein levels of the MC marker tryptase and TAM marker CD206 were evaluated with immunohistochemistry (IHC). The correlation between MC infiltration and prognostic factors was evaluated. The effects of MCs on the malignant potential were examined using in vitro proliferation and invasion assays with a colorectal cancer (CRC) cell line (HCT-116). Following nCRT, 31.6% of resected LARC patient specimens were positive for MC infiltration by tryptase IHC analysis. MC infiltration was significantly correlated with nCRT response. The 5-year disease-free survival (DFS) rate was significantly lower in the MC-positive group compared with the MC-negative group (52.3% vs. 76.8%). Univariate and multivariate analyses revealed that MC infiltration was the independent prognostic indicator for DFS. MC infiltration was significantly correlated with CD206 expression, and therefore TAMs. In vitro experiments suggested that tumor activated mast cells could promote CRC cell malignant behavior via production of macrophage inhibitory factor. MC infiltration in LARC patients was positively correlated with TAM infiltration and resistance to nCRT, and was also an independent poor prognostic indicator.https://doi.org/10.1186/s12885-025-13458-9Mast cellRectal cancerNeoadjuvant chemoradiotherapyTumor associated macrophage |
| spellingShingle | Masaaki Nishi Shoko Yamashita Chie Takasu Yuma Wada Kozo Yoshikawa Takuya Tokunaga Toshihiro Nakao Hideya Kashihara Toshiaki Yoshimoto Mitsuo Shimada Role of mast cell in locally advanced rectal cancer treated with neoadjuvant chemoradiotherapy BMC Cancer Mast cell Rectal cancer Neoadjuvant chemoradiotherapy Tumor associated macrophage |
| title | Role of mast cell in locally advanced rectal cancer treated with neoadjuvant chemoradiotherapy |
| title_full | Role of mast cell in locally advanced rectal cancer treated with neoadjuvant chemoradiotherapy |
| title_fullStr | Role of mast cell in locally advanced rectal cancer treated with neoadjuvant chemoradiotherapy |
| title_full_unstemmed | Role of mast cell in locally advanced rectal cancer treated with neoadjuvant chemoradiotherapy |
| title_short | Role of mast cell in locally advanced rectal cancer treated with neoadjuvant chemoradiotherapy |
| title_sort | role of mast cell in locally advanced rectal cancer treated with neoadjuvant chemoradiotherapy |
| topic | Mast cell Rectal cancer Neoadjuvant chemoradiotherapy Tumor associated macrophage |
| url | https://doi.org/10.1186/s12885-025-13458-9 |
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