Study of RNA Interference Targeting NET-1 Combination with Sorafenib for Hepatocellular Carcinoma Therapy In Vitro and In Vivo
The aim of this study is to explore the inhibitory effects of RNA interference (RNAi) targeting NET-1 or combined with sorafenib on HCC in vitro and in vivo and the possible underlying mechanisms. The expressions of NET-1 mRNA and protein were detected by RT-QPCR and western blot. The ability of pro...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Gastroenterology Research and Practice |
| Online Access: | http://dx.doi.org/10.1155/2013/685150 |
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| author | Song He Ying-ze Wei Gui-lan Wang Yu-yin Xu Jia-ming Zhou Yi-xin Zhang Li Chen |
| author_facet | Song He Ying-ze Wei Gui-lan Wang Yu-yin Xu Jia-ming Zhou Yi-xin Zhang Li Chen |
| author_sort | Song He |
| collection | DOAJ |
| description | The aim of this study is to explore the inhibitory effects of RNA interference (RNAi) targeting NET-1 or combined with sorafenib on HCC in vitro and in vivo and the possible underlying mechanisms. The expressions of NET-1 mRNA and protein were detected by RT-QPCR and western blot. The ability of proliferation was determined by CCK-8 assay. Apoptosis was examined by flow cytometry (FCM). Abilities of migration and invasion were measured by scratch-wound assay and transwell assay. MHCC97H cells with stable transfection of NET-1shRNA were injected subcutaneously to prepare nude mice model of HCC and Caspase-3, Caspase-8, and Caspase-9 mRNAs of tumor tissues in different groups were examined. NET-1 mRNA and protein were reduced sharply in MHCC97H cells transfected with NET-1shRNA. The abilities of proliferation and migration were inhibited and apoptosis was promoted in either NET-1shRNA or sorafenib as compared with untreated cells in vitro and in vivo (P<0.05). The mRNA levels of caspase-3, caspase-8, and caspase-9 of tumor tissues were reduced in different treatment groups compared with untreated group, particularly in combination group. (P<0.05). The combination NET-1shRNA with sorafenib dramatically enhanced the effects of sorafenib antitumor ,which may involve in blocking ras signaling pathway and stimulating apoptotic pathways simultaneously. |
| format | Article |
| id | doaj-art-d4ff95c2ee564ab480befc6113750a74 |
| institution | Kabale University |
| issn | 1687-6121 1687-630X |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Gastroenterology Research and Practice |
| spelling | doaj-art-d4ff95c2ee564ab480befc6113750a742025-02-03T01:00:53ZengWileyGastroenterology Research and Practice1687-61211687-630X2013-01-01201310.1155/2013/685150685150Study of RNA Interference Targeting NET-1 Combination with Sorafenib for Hepatocellular Carcinoma Therapy In Vitro and In VivoSong He0Ying-ze Wei1Gui-lan Wang2Yu-yin Xu3Jia-ming Zhou4Yi-xin Zhang5Li Chen6Department of Pathology, Nantong Tumor Hospital, Nantong 226361, ChinaDepartment of Pathology, Nantong Tumor Hospital, Nantong 226361, ChinaDepartment of Pathological Anatomy, Nantong University, Nantong 226001, ChinaDepartment of Pathological Anatomy, Nantong University, Nantong 226001, ChinaDepartment of Pathological Anatomy, Nantong University, Nantong 226001, ChinaDepartment of Pathology, Nantong Tumor Hospital, Nantong 226361, ChinaDepartment of Pathology, Nantong Tumor Hospital, Nantong 226361, ChinaThe aim of this study is to explore the inhibitory effects of RNA interference (RNAi) targeting NET-1 or combined with sorafenib on HCC in vitro and in vivo and the possible underlying mechanisms. The expressions of NET-1 mRNA and protein were detected by RT-QPCR and western blot. The ability of proliferation was determined by CCK-8 assay. Apoptosis was examined by flow cytometry (FCM). Abilities of migration and invasion were measured by scratch-wound assay and transwell assay. MHCC97H cells with stable transfection of NET-1shRNA were injected subcutaneously to prepare nude mice model of HCC and Caspase-3, Caspase-8, and Caspase-9 mRNAs of tumor tissues in different groups were examined. NET-1 mRNA and protein were reduced sharply in MHCC97H cells transfected with NET-1shRNA. The abilities of proliferation and migration were inhibited and apoptosis was promoted in either NET-1shRNA or sorafenib as compared with untreated cells in vitro and in vivo (P<0.05). The mRNA levels of caspase-3, caspase-8, and caspase-9 of tumor tissues were reduced in different treatment groups compared with untreated group, particularly in combination group. (P<0.05). The combination NET-1shRNA with sorafenib dramatically enhanced the effects of sorafenib antitumor ,which may involve in blocking ras signaling pathway and stimulating apoptotic pathways simultaneously.http://dx.doi.org/10.1155/2013/685150 |
| spellingShingle | Song He Ying-ze Wei Gui-lan Wang Yu-yin Xu Jia-ming Zhou Yi-xin Zhang Li Chen Study of RNA Interference Targeting NET-1 Combination with Sorafenib for Hepatocellular Carcinoma Therapy In Vitro and In Vivo Gastroenterology Research and Practice |
| title | Study of RNA Interference Targeting NET-1 Combination with Sorafenib for Hepatocellular Carcinoma Therapy In Vitro and In Vivo |
| title_full | Study of RNA Interference Targeting NET-1 Combination with Sorafenib for Hepatocellular Carcinoma Therapy In Vitro and In Vivo |
| title_fullStr | Study of RNA Interference Targeting NET-1 Combination with Sorafenib for Hepatocellular Carcinoma Therapy In Vitro and In Vivo |
| title_full_unstemmed | Study of RNA Interference Targeting NET-1 Combination with Sorafenib for Hepatocellular Carcinoma Therapy In Vitro and In Vivo |
| title_short | Study of RNA Interference Targeting NET-1 Combination with Sorafenib for Hepatocellular Carcinoma Therapy In Vitro and In Vivo |
| title_sort | study of rna interference targeting net 1 combination with sorafenib for hepatocellular carcinoma therapy in vitro and in vivo |
| url | http://dx.doi.org/10.1155/2013/685150 |
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