Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouse
lncRNAs are acquiring increasing relevance as regulators in a wide spectrum of biological processes. The extreme heterogeneity in the mechanisms of action of these molecules, however, makes them very difficult to study, especially regarding their molecular function. A novel lncRNA has been recently...
Saved in:
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | International Journal of Genomics |
| Online Access: | http://dx.doi.org/10.1155/2017/9769171 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554863727214592 |
|---|---|
| author | Sara Carmela Credendino Nicole Lewin Miriane de Oliveira Swaraj Basu Barbara D’Andrea Elena Amendola Luigi Di Guida Antonio Nardone Remo Sanges Mario De Felice Gabriella De Vita |
| author_facet | Sara Carmela Credendino Nicole Lewin Miriane de Oliveira Swaraj Basu Barbara D’Andrea Elena Amendola Luigi Di Guida Antonio Nardone Remo Sanges Mario De Felice Gabriella De Vita |
| author_sort | Sara Carmela Credendino |
| collection | DOAJ |
| description | lncRNAs are acquiring increasing relevance as regulators in a wide spectrum of biological processes. The extreme heterogeneity in the mechanisms of action of these molecules, however, makes them very difficult to study, especially regarding their molecular function. A novel lncRNA has been recently identified as the most enriched transcript in mouse developing thyroid. Due to its genomic localization antisense to the protein-encoding Klhl14 gene, we named it Klhl14-AS. In this paper, we highlight that mouse Klhl14-AS produces at least five splicing variants, some of which have not been previously described. Klhl14-AS is expressed with a peculiar pattern, characterized by diverse relative abundance of its isoforms in different mouse tissues. We examine the whole expression level of Klhl14-AS in a panel of adult mouse tissues, showing that it is expressed in the thyroid, lung, kidney, testis, ovary, brain, and spleen, although at different levels. In situ hybridization analysis reveals that, in the context of each organ, Klhl14-AS shows a cell type-specific expression. Interestingly, databases report a similar expression profile for human Klhl14-AS. Our observations suggest that this lncRNA could play cell type-specific roles in several organs and pave the way for functional characterization of this gene in appropriate biological contexts. |
| format | Article |
| id | doaj-art-d4e975f2fec64975b68919f1e3dd939b |
| institution | Kabale University |
| issn | 2314-436X 2314-4378 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Genomics |
| spelling | doaj-art-d4e975f2fec64975b68919f1e3dd939b2025-02-03T05:50:25ZengWileyInternational Journal of Genomics2314-436X2314-43782017-01-01201710.1155/2017/97691719769171Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in MouseSara Carmela Credendino0Nicole Lewin1Miriane de Oliveira2Swaraj Basu3Barbara D’Andrea4Elena Amendola5Luigi Di Guida6Antonio Nardone7Remo Sanges8Mario De Felice9Gabriella De Vita10Dipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, ItalyDipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, ItalyDipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, ItalyDepartment of Medical Biochemistry and Cell Biology, Institute of Biomedicine, the Sahlgrenska Academy, University of Gothenburg, Gothenburg, SwedenDipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, ItalyDipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, ItalyDipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, ItalyDipartimento di Sanità Pubblica, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, ItalyBiology and Evolution of Marine Organisms, Stazione Zoologica Anton Dohrn, Villa Comunale 1, 80121 Napoli, ItalyDipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, ItalyDipartimento di Medicina Molecolare e Biotecnologie Mediche, Università degli Studi di Napoli Federico II, Via S. Pansini 5, 80131 Napoli, ItalylncRNAs are acquiring increasing relevance as regulators in a wide spectrum of biological processes. The extreme heterogeneity in the mechanisms of action of these molecules, however, makes them very difficult to study, especially regarding their molecular function. A novel lncRNA has been recently identified as the most enriched transcript in mouse developing thyroid. Due to its genomic localization antisense to the protein-encoding Klhl14 gene, we named it Klhl14-AS. In this paper, we highlight that mouse Klhl14-AS produces at least five splicing variants, some of which have not been previously described. Klhl14-AS is expressed with a peculiar pattern, characterized by diverse relative abundance of its isoforms in different mouse tissues. We examine the whole expression level of Klhl14-AS in a panel of adult mouse tissues, showing that it is expressed in the thyroid, lung, kidney, testis, ovary, brain, and spleen, although at different levels. In situ hybridization analysis reveals that, in the context of each organ, Klhl14-AS shows a cell type-specific expression. Interestingly, databases report a similar expression profile for human Klhl14-AS. Our observations suggest that this lncRNA could play cell type-specific roles in several organs and pave the way for functional characterization of this gene in appropriate biological contexts.http://dx.doi.org/10.1155/2017/9769171 |
| spellingShingle | Sara Carmela Credendino Nicole Lewin Miriane de Oliveira Swaraj Basu Barbara D’Andrea Elena Amendola Luigi Di Guida Antonio Nardone Remo Sanges Mario De Felice Gabriella De Vita Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouse International Journal of Genomics |
| title | Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouse |
| title_full | Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouse |
| title_fullStr | Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouse |
| title_full_unstemmed | Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouse |
| title_short | Tissue- and Cell Type-Specific Expression of the Long Noncoding RNA Klhl14-AS in Mouse |
| title_sort | tissue and cell type specific expression of the long noncoding rna klhl14 as in mouse |
| url | http://dx.doi.org/10.1155/2017/9769171 |
| work_keys_str_mv | AT saracarmelacredendino tissueandcelltypespecificexpressionofthelongnoncodingrnaklhl14asinmouse AT nicolelewin tissueandcelltypespecificexpressionofthelongnoncodingrnaklhl14asinmouse AT mirianedeoliveira tissueandcelltypespecificexpressionofthelongnoncodingrnaklhl14asinmouse AT swarajbasu tissueandcelltypespecificexpressionofthelongnoncodingrnaklhl14asinmouse AT barbaradandrea tissueandcelltypespecificexpressionofthelongnoncodingrnaklhl14asinmouse AT elenaamendola tissueandcelltypespecificexpressionofthelongnoncodingrnaklhl14asinmouse AT luigidiguida tissueandcelltypespecificexpressionofthelongnoncodingrnaklhl14asinmouse AT antonionardone tissueandcelltypespecificexpressionofthelongnoncodingrnaklhl14asinmouse AT remosanges tissueandcelltypespecificexpressionofthelongnoncodingrnaklhl14asinmouse AT mariodefelice tissueandcelltypespecificexpressionofthelongnoncodingrnaklhl14asinmouse AT gabrielladevita tissueandcelltypespecificexpressionofthelongnoncodingrnaklhl14asinmouse |