Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning
Persistent alteration of protein conformation due to interaction with isoflurane may be a novel molecular aspect of preconditioning. We preincubated human serum albumin with isoflurane, dialyzed to release agent, and assessed protein conformation. Susceptibility to chemical modification by methylgly...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Biochemistry Research International |
| Online Access: | http://dx.doi.org/10.1155/2011/739712 |
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| author | Michelle R. Baker Sean K. Benton Christopher S. Theisen Chad A. McClintick Eugene E. Fibuch Norbert W. Seidler |
| author_facet | Michelle R. Baker Sean K. Benton Christopher S. Theisen Chad A. McClintick Eugene E. Fibuch Norbert W. Seidler |
| author_sort | Michelle R. Baker |
| collection | DOAJ |
| description | Persistent alteration of protein conformation due to interaction with isoflurane may be a novel molecular aspect of preconditioning. We preincubated human serum albumin with isoflurane, dialyzed to release agent, and assessed protein conformation. Susceptibility to chemical modification by methylglyoxal and nitrophenylacetate was also examined. Isoflurane had a persistent effect on protein conformation. An increase in the susceptibility of surface residues to chemical modification attended this change in conformation. Modification of isoflurane-treated HSA included intra- and intersubunit cross-linking that may be a consequence of anesthetic-induced changes in multimeric subpopulations. This irreversible effect of isoflurane may represent a mechanism for preconditioning. |
| format | Article |
| id | doaj-art-d4b91e5d70894f95925704f08bf21d20 |
| institution | Kabale University |
| issn | 2090-2247 2090-2255 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Biochemistry Research International |
| spelling | doaj-art-d4b91e5d70894f95925704f08bf21d202025-02-03T05:51:18ZengWileyBiochemistry Research International2090-22472090-22552011-01-01201110.1155/2011/739712739712Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for PreconditioningMichelle R. Baker0Sean K. Benton1Christopher S. Theisen2Chad A. McClintick3Eugene E. Fibuch4Norbert W. Seidler5Department of Anesthesiology, University of Missouri-Kansas City School of Medicine, 4401 Wornall Road, Kansas City, MO 64108, USADepartment of Anesthesiology, University of Missouri-Kansas City School of Medicine, 4401 Wornall Road, Kansas City, MO 64108, USADepartment of Biochemistry, Kansas City University of Medicine and Biosciences, 1750 Independence Avenue, Kansas City, MO 64106, USADepartment of Anesthesiology, University of Missouri-Kansas City School of Medicine, 4401 Wornall Road, Kansas City, MO 64108, USADepartment of Anesthesiology, University of Missouri-Kansas City School of Medicine, 4401 Wornall Road, Kansas City, MO 64108, USADepartment of Biochemistry, Kansas City University of Medicine and Biosciences, 1750 Independence Avenue, Kansas City, MO 64106, USAPersistent alteration of protein conformation due to interaction with isoflurane may be a novel molecular aspect of preconditioning. We preincubated human serum albumin with isoflurane, dialyzed to release agent, and assessed protein conformation. Susceptibility to chemical modification by methylglyoxal and nitrophenylacetate was also examined. Isoflurane had a persistent effect on protein conformation. An increase in the susceptibility of surface residues to chemical modification attended this change in conformation. Modification of isoflurane-treated HSA included intra- and intersubunit cross-linking that may be a consequence of anesthetic-induced changes in multimeric subpopulations. This irreversible effect of isoflurane may represent a mechanism for preconditioning.http://dx.doi.org/10.1155/2011/739712 |
| spellingShingle | Michelle R. Baker Sean K. Benton Christopher S. Theisen Chad A. McClintick Eugene E. Fibuch Norbert W. Seidler Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning Biochemistry Research International |
| title | Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning |
| title_full | Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning |
| title_fullStr | Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning |
| title_full_unstemmed | Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning |
| title_short | Isoflurane's Effect on Protein Conformation as a Proposed Mechanism for Preconditioning |
| title_sort | isoflurane s effect on protein conformation as a proposed mechanism for preconditioning |
| url | http://dx.doi.org/10.1155/2011/739712 |
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