Bacterial blood microbiome of Mastomys rodents: implications for disease spill-over at the animal-human interface within the Bushbuckridge-East community, South Africa
The Bushbuckridge-East community in Mpumalanga Province, South Africa is bordered by nature reserves, including the Manyeleti Game Reserve. Murid rodents are prevalent in both Manyeleti and communal rangelands adjoining the community households. Although rodents are reservoir hosts for a broad range...
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Frontiers Media S.A.
2025-02-01
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| Series: | Frontiers in Cellular and Infection Microbiology |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1520086/full |
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| author | Agatha O. Kolo Agatha O. Kolo Kelly A. Brayton Kelly A. Brayton Nicola E. Collins Armanda D. S. Bastos Armanda D. S. Bastos Armanda D. S. Bastos Sonja Matthee Cory A. Gall Jeanette Wentzel Jeanette Wentzel Luis Neves Luis Neves Marinda C. Oosthuizen |
| author_facet | Agatha O. Kolo Agatha O. Kolo Kelly A. Brayton Kelly A. Brayton Nicola E. Collins Armanda D. S. Bastos Armanda D. S. Bastos Armanda D. S. Bastos Sonja Matthee Cory A. Gall Jeanette Wentzel Jeanette Wentzel Luis Neves Luis Neves Marinda C. Oosthuizen |
| author_sort | Agatha O. Kolo |
| collection | DOAJ |
| description | The Bushbuckridge-East community in Mpumalanga Province, South Africa is bordered by nature reserves, including the Manyeleti Game Reserve. Murid rodents are prevalent in both Manyeleti and communal rangelands adjoining the community households. Although rodents are reservoir hosts for a broad range of viral, bacterial and parasitic pathogens, the rodent microbial diversity and transmission of zoonotic agents to humans in the community is understudied. In this study we investigated bacterial diversity in wild and commensal rodents sampled from different habitats. The 16S rRNA gene was amplified from DNA extracted from the blood of 24 wild Mastomys and one Steatomys sp. and subjected to PacBio circular consensus sequencing. As Bartonella species were dominant in the blood microbiome, gltA gene characterization was performed to delineate species. Rodents sampled from peri-urban and communal rangelands had higher proportions of Bartonella spp. [Hlalakahle (77.7%), Gottenburg (47.8%), Tlhavekisa (83.8%)] compared to those from the protected habitat (43.8%). Ehrlichia spp., Anaplasma spp., and Coxiella burnetii were detected at <1% of the sequence reads. Conventional PCR and sequencing validated the detection of Bartonella spp. with the first confirmation of Bartonella mastomydis infection in Mastomys in South Africa. Additionally, 317 mites, 90 fleas, 10 ticks and eight lice were collected from the rodents, providing evidence of possible vectors of the organisms detected. The detection of zoonotic agents in rodents in Bushbuckridge-East community, together with prior serological confirmation of Bartonella and Coxiella in non-malarial acute febrile patients from this community, highlights the possible risks that commensal rodents pose to human health. |
| format | Article |
| id | doaj-art-d4b2e6ffece041fcaec1b2e160f8abf9 |
| institution | Kabale University |
| issn | 2235-2988 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Cellular and Infection Microbiology |
| spelling | doaj-art-d4b2e6ffece041fcaec1b2e160f8abf92025-02-03T06:33:43ZengFrontiers Media S.A.Frontiers in Cellular and Infection Microbiology2235-29882025-02-011510.3389/fcimb.2025.15200861520086Bacterial blood microbiome of Mastomys rodents: implications for disease spill-over at the animal-human interface within the Bushbuckridge-East community, South AfricaAgatha O. Kolo0Agatha O. Kolo1Kelly A. Brayton2Kelly A. Brayton3Nicola E. Collins4Armanda D. S. Bastos5Armanda D. S. Bastos6Armanda D. S. Bastos7Sonja Matthee8Cory A. Gall9Jeanette Wentzel10Jeanette Wentzel11Luis Neves12Luis Neves13Marinda C. Oosthuizen14Department of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South AfricaDepartment of Molecular Microbiology and Immunology, The University of Texas at San Antonio, San Antonio, TX, United StatesDepartment of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South AfricaDepartment of Veterinary Microbiology and Pathology, Washington State University, Pullman, WA, United StatesDepartment of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South AfricaDepartment of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South AfricaDepartment of Zoology and Entomology, Mammal Research Institute, University of Pretoria, Pretoria, South AfricaHans Hoheisen Research Centre, Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Pretoria, South AfricaDepartment of Conservation Ecology and Entomology, Stellenbosch University, Matieland, South AfricaDepartment of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South AfricaDepartment of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South AfricaHans Hoheisen Research Centre, Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Pretoria, South AfricaDepartment of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South AfricaCentro de Biotecnologia, Eduardo Mondlane University, Maputo, MozambiqueDepartment of Veterinary Tropical Diseases, University of Pretoria, Pretoria, South AfricaThe Bushbuckridge-East community in Mpumalanga Province, South Africa is bordered by nature reserves, including the Manyeleti Game Reserve. Murid rodents are prevalent in both Manyeleti and communal rangelands adjoining the community households. Although rodents are reservoir hosts for a broad range of viral, bacterial and parasitic pathogens, the rodent microbial diversity and transmission of zoonotic agents to humans in the community is understudied. In this study we investigated bacterial diversity in wild and commensal rodents sampled from different habitats. The 16S rRNA gene was amplified from DNA extracted from the blood of 24 wild Mastomys and one Steatomys sp. and subjected to PacBio circular consensus sequencing. As Bartonella species were dominant in the blood microbiome, gltA gene characterization was performed to delineate species. Rodents sampled from peri-urban and communal rangelands had higher proportions of Bartonella spp. [Hlalakahle (77.7%), Gottenburg (47.8%), Tlhavekisa (83.8%)] compared to those from the protected habitat (43.8%). Ehrlichia spp., Anaplasma spp., and Coxiella burnetii were detected at <1% of the sequence reads. Conventional PCR and sequencing validated the detection of Bartonella spp. with the first confirmation of Bartonella mastomydis infection in Mastomys in South Africa. Additionally, 317 mites, 90 fleas, 10 ticks and eight lice were collected from the rodents, providing evidence of possible vectors of the organisms detected. The detection of zoonotic agents in rodents in Bushbuckridge-East community, together with prior serological confirmation of Bartonella and Coxiella in non-malarial acute febrile patients from this community, highlights the possible risks that commensal rodents pose to human health.https://www.frontiersin.org/articles/10.3389/fcimb.2025.1520086/fullBartonellaEhrlichiaAnaplasmaCoxiella burnetii16S rRNA genegltA gene |
| spellingShingle | Agatha O. Kolo Agatha O. Kolo Kelly A. Brayton Kelly A. Brayton Nicola E. Collins Armanda D. S. Bastos Armanda D. S. Bastos Armanda D. S. Bastos Sonja Matthee Cory A. Gall Jeanette Wentzel Jeanette Wentzel Luis Neves Luis Neves Marinda C. Oosthuizen Bacterial blood microbiome of Mastomys rodents: implications for disease spill-over at the animal-human interface within the Bushbuckridge-East community, South Africa Frontiers in Cellular and Infection Microbiology Bartonella Ehrlichia Anaplasma Coxiella burnetii 16S rRNA gene gltA gene |
| title | Bacterial blood microbiome of Mastomys rodents: implications for disease spill-over at the animal-human interface within the Bushbuckridge-East community, South Africa |
| title_full | Bacterial blood microbiome of Mastomys rodents: implications for disease spill-over at the animal-human interface within the Bushbuckridge-East community, South Africa |
| title_fullStr | Bacterial blood microbiome of Mastomys rodents: implications for disease spill-over at the animal-human interface within the Bushbuckridge-East community, South Africa |
| title_full_unstemmed | Bacterial blood microbiome of Mastomys rodents: implications for disease spill-over at the animal-human interface within the Bushbuckridge-East community, South Africa |
| title_short | Bacterial blood microbiome of Mastomys rodents: implications for disease spill-over at the animal-human interface within the Bushbuckridge-East community, South Africa |
| title_sort | bacterial blood microbiome of mastomys rodents implications for disease spill over at the animal human interface within the bushbuckridge east community south africa |
| topic | Bartonella Ehrlichia Anaplasma Coxiella burnetii 16S rRNA gene gltA gene |
| url | https://www.frontiersin.org/articles/10.3389/fcimb.2025.1520086/full |
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