Effects of Severe Hypoxia on Bone Marrow Mesenchymal Stem Cells Differentiation Potential
Background. The interests in mesenchymal stem cells (MSCs) and their application in cell therapy have resulted in a better understanding of the basic biology of these cells. Recently hypoxia has been indicated as crucial for complete chondrogenesis. We aimed at analyzing bone marrow MSCs (BM-MSCs) d...
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| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2013/232896 |
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| author | Claudia Cicione Emma Muiños-López Tamara Hermida-Gómez Isaac Fuentes-Boquete Silvia Díaz-Prado Francisco J. Blanco |
| author_facet | Claudia Cicione Emma Muiños-López Tamara Hermida-Gómez Isaac Fuentes-Boquete Silvia Díaz-Prado Francisco J. Blanco |
| author_sort | Claudia Cicione |
| collection | DOAJ |
| description | Background. The interests in mesenchymal stem cells (MSCs) and their application in cell therapy have resulted in a better understanding of the basic biology of these cells. Recently hypoxia has been indicated as crucial for complete chondrogenesis. We aimed at analyzing bone marrow MSCs (BM-MSCs) differentiation capacity under normoxic and severe hypoxic culture conditions. Methods. MSCs were characterized by flow cytometry and differentiated towards adipocytes, osteoblasts, and chondrocytes under normoxic or severe hypoxic conditions. The differentiations were confirmed comparing each treated point with a control point made of cells grown in DMEM and fetal bovine serum (FBS). Results. BM-MSCs from the donors displayed only few phenotypical differences in surface antigens expressions. Analyzing marker genes expression levels of the treated cells compared to their control point for each lineage showed a good differentiation in normoxic conditions and the absence of this differentiation capacity in severe hypoxic cultures. Conclusions. In our experimental conditions, severe hypoxia affects the in vitro differentiation potential of BM-MSCs. Adipogenic, osteogenic, and chondrogenic differentiations are absent in severe hypoxic conditions. Our work underlines that severe hypoxia slows cell differentiation by means of molecular mechanisms since a decrease in the expression of adipocyte-, osteoblast-, and chondrocyte-specific genes was observed. |
| format | Article |
| id | doaj-art-d4afcb388cc245988dd3f0483c31cb75 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-d4afcb388cc245988dd3f0483c31cb752025-02-03T01:20:57ZengWileyStem Cells International1687-966X1687-96782013-01-01201310.1155/2013/232896232896Effects of Severe Hypoxia on Bone Marrow Mesenchymal Stem Cells Differentiation PotentialClaudia Cicione0Emma Muiños-López1Tamara Hermida-Gómez2Isaac Fuentes-Boquete3Silvia Díaz-Prado4Francisco J. Blanco5Rheumatology Division, INIBIC Hospital Universitario A Coruña, C/As Xubias S/N, 15006 A Coruña, SpainRheumatology Division, INIBIC Hospital Universitario A Coruña, C/As Xubias S/N, 15006 A Coruña, SpainRheumatology Division, INIBIC Hospital Universitario A Coruña, C/As Xubias S/N, 15006 A Coruña, SpainCIBER-BBN-Cellular Therapy Area, Hospital Universitario A Coruña, C/As Xubias S/N, 15006 A Coruña, SpainCIBER-BBN-Cellular Therapy Area, Hospital Universitario A Coruña, C/As Xubias S/N, 15006 A Coruña, SpainRheumatology Division, INIBIC Hospital Universitario A Coruña, C/As Xubias S/N, 15006 A Coruña, SpainBackground. The interests in mesenchymal stem cells (MSCs) and their application in cell therapy have resulted in a better understanding of the basic biology of these cells. Recently hypoxia has been indicated as crucial for complete chondrogenesis. We aimed at analyzing bone marrow MSCs (BM-MSCs) differentiation capacity under normoxic and severe hypoxic culture conditions. Methods. MSCs were characterized by flow cytometry and differentiated towards adipocytes, osteoblasts, and chondrocytes under normoxic or severe hypoxic conditions. The differentiations were confirmed comparing each treated point with a control point made of cells grown in DMEM and fetal bovine serum (FBS). Results. BM-MSCs from the donors displayed only few phenotypical differences in surface antigens expressions. Analyzing marker genes expression levels of the treated cells compared to their control point for each lineage showed a good differentiation in normoxic conditions and the absence of this differentiation capacity in severe hypoxic cultures. Conclusions. In our experimental conditions, severe hypoxia affects the in vitro differentiation potential of BM-MSCs. Adipogenic, osteogenic, and chondrogenic differentiations are absent in severe hypoxic conditions. Our work underlines that severe hypoxia slows cell differentiation by means of molecular mechanisms since a decrease in the expression of adipocyte-, osteoblast-, and chondrocyte-specific genes was observed.http://dx.doi.org/10.1155/2013/232896 |
| spellingShingle | Claudia Cicione Emma Muiños-López Tamara Hermida-Gómez Isaac Fuentes-Boquete Silvia Díaz-Prado Francisco J. Blanco Effects of Severe Hypoxia on Bone Marrow Mesenchymal Stem Cells Differentiation Potential Stem Cells International |
| title | Effects of Severe Hypoxia on Bone Marrow Mesenchymal Stem Cells Differentiation Potential |
| title_full | Effects of Severe Hypoxia on Bone Marrow Mesenchymal Stem Cells Differentiation Potential |
| title_fullStr | Effects of Severe Hypoxia on Bone Marrow Mesenchymal Stem Cells Differentiation Potential |
| title_full_unstemmed | Effects of Severe Hypoxia on Bone Marrow Mesenchymal Stem Cells Differentiation Potential |
| title_short | Effects of Severe Hypoxia on Bone Marrow Mesenchymal Stem Cells Differentiation Potential |
| title_sort | effects of severe hypoxia on bone marrow mesenchymal stem cells differentiation potential |
| url | http://dx.doi.org/10.1155/2013/232896 |
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