Protocol for fast antibiotic resistance-based gene editing of mammalian cells with CRISPR-Cas9

Protocol for fast antibiotic resistance-based gene editing of mammalian cells with CRISPR-Cas9

Summary: Protein tagging with CRISPR-Cas9 enables the investigation of protein function in its native environment but is limited by low homology-directed repair (HDR) efficiency. Here, we present a protocol for fast antibiotic resistance-based gene editing with CRISPR-Cas9 (FAB-CRISPR), which stream...

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Bibliographic Details
Main Authors: Petia Adarska, Eleanor Fox, Joshua Heyza, Carlo Barnaba, Jens Schmidt, Francesca Bottanelli
Format: Article
Language:English
Published: Elsevier 2025-09-01
Series:STAR Protocols
Subjects:
Cell Biology
Molecular Biology
CRISPR
Online Access:http://www.sciencedirect.com/science/article/pii/S2666166725003557
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