CYP Gene Expression and In Vivo Biological Effects of Khat Ethanol Extract, Cathinone and Cathine in Caenorhabditis elegans
The significant influence of khat, cathinone, and cathine on appetite suppression, reproduction impairment, and inhibitory effects on CYPs enzyme activity is widely recognised, but much remains unknown about its mode of action. This study demonstrated that exposure to high doses of KEE, cathinone,...
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| Format: | Article |
| Language: | English |
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HH Publisher
2023-09-01
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| Series: | Progress in Microbes and Molecular Biology |
| Online Access: | https://journals.hh-publisher.com/index.php/pmmb/article/view/855 |
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| author | Sharoen Yu Ming Lim Mohammed Abdullah Alshawsh Mustafa Alshagga Cin Kong Chee-Mun Fang Yan Pan |
| author_facet | Sharoen Yu Ming Lim Mohammed Abdullah Alshawsh Mustafa Alshagga Cin Kong Chee-Mun Fang Yan Pan |
| author_sort | Sharoen Yu Ming Lim |
| collection | DOAJ |
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The significant influence of khat, cathinone, and cathine on appetite suppression, reproduction impairment, and inhibitory effects on CYPs enzyme activity is widely recognised, but much remains unknown about its mode of action. This study demonstrated that exposure to high doses of KEE, cathinone, cathine and sibutramine at 12.5, 1.8, 1.8 and 1.8 mg/ml showed the most visible reduction in pharyngeal pumping rate, respectively. C. elegans showed an anomalous dynamic of reproduction rate upon exposure to these compounds but overall, showed a reduced amount of progeny. KEE, cathinone, and cathine extended the lifespan of C. elegans as compared to sibutramine and control with survival rate increments of 59.5%, 43.3%, and 15%, respectively. Quantitative RT-PCR results were insignificant, but cyp-14A3, cyp-34A9, and cyp-35A2 across all treatments were upregulated, ranging from >1 to 3-fold as compared to control. cyp genes expression could be upregulated to enhance the metabolism of khat extract, cathinone, cathine, and sibutramine in C. elegans.
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| format | Article |
| id | doaj-art-d4985936a619467b8fd660d5edfe0457 |
| institution | Kabale University |
| issn | 2637-1049 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | HH Publisher |
| record_format | Article |
| series | Progress in Microbes and Molecular Biology |
| spelling | doaj-art-d4985936a619467b8fd660d5edfe04572025-02-04T08:39:49ZengHH PublisherProgress in Microbes and Molecular Biology2637-10492023-09-016110.36877/pmmb.a0000344CYP Gene Expression and In Vivo Biological Effects of Khat Ethanol Extract, Cathinone and Cathine in Caenorhabditis elegansSharoen Yu Ming LimMohammed Abdullah AlshawshMustafa AlshaggaCin KongChee-Mun FangYan Pan The significant influence of khat, cathinone, and cathine on appetite suppression, reproduction impairment, and inhibitory effects on CYPs enzyme activity is widely recognised, but much remains unknown about its mode of action. This study demonstrated that exposure to high doses of KEE, cathinone, cathine and sibutramine at 12.5, 1.8, 1.8 and 1.8 mg/ml showed the most visible reduction in pharyngeal pumping rate, respectively. C. elegans showed an anomalous dynamic of reproduction rate upon exposure to these compounds but overall, showed a reduced amount of progeny. KEE, cathinone, and cathine extended the lifespan of C. elegans as compared to sibutramine and control with survival rate increments of 59.5%, 43.3%, and 15%, respectively. Quantitative RT-PCR results were insignificant, but cyp-14A3, cyp-34A9, and cyp-35A2 across all treatments were upregulated, ranging from >1 to 3-fold as compared to control. cyp genes expression could be upregulated to enhance the metabolism of khat extract, cathinone, cathine, and sibutramine in C. elegans. https://journals.hh-publisher.com/index.php/pmmb/article/view/855 |
| spellingShingle | Sharoen Yu Ming Lim Mohammed Abdullah Alshawsh Mustafa Alshagga Cin Kong Chee-Mun Fang Yan Pan CYP Gene Expression and In Vivo Biological Effects of Khat Ethanol Extract, Cathinone and Cathine in Caenorhabditis elegans Progress in Microbes and Molecular Biology |
| title | CYP Gene Expression and In Vivo Biological Effects of Khat Ethanol Extract, Cathinone and Cathine in Caenorhabditis elegans |
| title_full | CYP Gene Expression and In Vivo Biological Effects of Khat Ethanol Extract, Cathinone and Cathine in Caenorhabditis elegans |
| title_fullStr | CYP Gene Expression and In Vivo Biological Effects of Khat Ethanol Extract, Cathinone and Cathine in Caenorhabditis elegans |
| title_full_unstemmed | CYP Gene Expression and In Vivo Biological Effects of Khat Ethanol Extract, Cathinone and Cathine in Caenorhabditis elegans |
| title_short | CYP Gene Expression and In Vivo Biological Effects of Khat Ethanol Extract, Cathinone and Cathine in Caenorhabditis elegans |
| title_sort | cyp gene expression and in vivo biological effects of khat ethanol extract cathinone and cathine in caenorhabditis elegans |
| url | https://journals.hh-publisher.com/index.php/pmmb/article/view/855 |
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