A Comparative Study of the Metabolic and Skeletal Response of C57BL/6J and C57BL/6N Mice in a Diet-Induced Model of Type 2 Diabetes
Type 2 diabetes mellitus (T2DM) represents a complex clinical scenario of altered energy metabolism and increased fracture incidence. The C57BL/6 mouse model of diet-induced obesity has been used to study the mechanisms by which altered glucose homeostasis affects bone mass and quality, but genetic...
Saved in:
| Main Authors: | , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2015-01-01
|
| Series: | Journal of Nutrition and Metabolism |
| Online Access: | http://dx.doi.org/10.1155/2015/758080 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832554460442787840 |
|---|---|
| author | Elizabeth Rendina-Ruedy Kelsey D. Hembree Angela Sasaki McKale R. Davis Stan A. Lightfoot Stephen L. Clarke Edralin A. Lucas Brenda J. Smith |
| author_facet | Elizabeth Rendina-Ruedy Kelsey D. Hembree Angela Sasaki McKale R. Davis Stan A. Lightfoot Stephen L. Clarke Edralin A. Lucas Brenda J. Smith |
| author_sort | Elizabeth Rendina-Ruedy |
| collection | DOAJ |
| description | Type 2 diabetes mellitus (T2DM) represents a complex clinical scenario of altered energy metabolism and increased fracture incidence. The C57BL/6 mouse model of diet-induced obesity has been used to study the mechanisms by which altered glucose homeostasis affects bone mass and quality, but genetic variations in substrains of C57BL/6 may have confounded data interpretation. This study investigated the long-term metabolic and skeletal consequences of two commonly used C57BL/6 substrains to a high fat (HF) diet. Male C57BL/6J, C57BL/6N, and the negative control strain, C3H/HeJ, mice were fed a control or HF diet for 24 wks. C57BL/6N mice on a HF diet demonstrated an increase in plasma insulin and blood glucose as early as 4 wk, whereas these responses were delayed in the C57BL/6J mice. The C57BL/6N mice exhibited more severe hepatic steatosis and inflammation. Only the C57BL/6N mice lost significant trabecular bone in response to the high fat diet. The C3H/HeJ mice were protected from bone loss. The data show that C57BL/6J and C57BL/6N mice differ in their metabolic and skeletal response when fed a HF diet. These substrain differences should be considered when designing experiments and are likely to have implications on data interpretation and reproducibility. |
| format | Article |
| id | doaj-art-d480beed32f84f32813fcb4b32624a4f |
| institution | Kabale University |
| issn | 2090-0724 2090-0732 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Nutrition and Metabolism |
| spelling | doaj-art-d480beed32f84f32813fcb4b32624a4f2025-02-03T05:51:27ZengWileyJournal of Nutrition and Metabolism2090-07242090-07322015-01-01201510.1155/2015/758080758080A Comparative Study of the Metabolic and Skeletal Response of C57BL/6J and C57BL/6N Mice in a Diet-Induced Model of Type 2 DiabetesElizabeth Rendina-Ruedy0Kelsey D. Hembree1Angela Sasaki2McKale R. Davis3Stan A. Lightfoot4Stephen L. Clarke5Edralin A. Lucas6Brenda J. Smith7Department of Nutritional Sciences, Oklahoma State University, Stillwater, OK 74078, USADepartment of Nutritional Sciences, Oklahoma State University, Stillwater, OK 74078, USADepartment of Nutritional Sciences, Oklahoma State University, Stillwater, OK 74078, USADepartment of Nutritional Sciences, Oklahoma State University, Stillwater, OK 74078, USACenter for Cancer Prevention and Drug Development, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USADepartment of Nutritional Sciences, Oklahoma State University, Stillwater, OK 74078, USADepartment of Nutritional Sciences, Oklahoma State University, Stillwater, OK 74078, USADepartment of Nutritional Sciences, Oklahoma State University, Stillwater, OK 74078, USAType 2 diabetes mellitus (T2DM) represents a complex clinical scenario of altered energy metabolism and increased fracture incidence. The C57BL/6 mouse model of diet-induced obesity has been used to study the mechanisms by which altered glucose homeostasis affects bone mass and quality, but genetic variations in substrains of C57BL/6 may have confounded data interpretation. This study investigated the long-term metabolic and skeletal consequences of two commonly used C57BL/6 substrains to a high fat (HF) diet. Male C57BL/6J, C57BL/6N, and the negative control strain, C3H/HeJ, mice were fed a control or HF diet for 24 wks. C57BL/6N mice on a HF diet demonstrated an increase in plasma insulin and blood glucose as early as 4 wk, whereas these responses were delayed in the C57BL/6J mice. The C57BL/6N mice exhibited more severe hepatic steatosis and inflammation. Only the C57BL/6N mice lost significant trabecular bone in response to the high fat diet. The C3H/HeJ mice were protected from bone loss. The data show that C57BL/6J and C57BL/6N mice differ in their metabolic and skeletal response when fed a HF diet. These substrain differences should be considered when designing experiments and are likely to have implications on data interpretation and reproducibility.http://dx.doi.org/10.1155/2015/758080 |
| spellingShingle | Elizabeth Rendina-Ruedy Kelsey D. Hembree Angela Sasaki McKale R. Davis Stan A. Lightfoot Stephen L. Clarke Edralin A. Lucas Brenda J. Smith A Comparative Study of the Metabolic and Skeletal Response of C57BL/6J and C57BL/6N Mice in a Diet-Induced Model of Type 2 Diabetes Journal of Nutrition and Metabolism |
| title | A Comparative Study of the Metabolic and Skeletal Response of C57BL/6J and C57BL/6N Mice in a Diet-Induced Model of Type 2 Diabetes |
| title_full | A Comparative Study of the Metabolic and Skeletal Response of C57BL/6J and C57BL/6N Mice in a Diet-Induced Model of Type 2 Diabetes |
| title_fullStr | A Comparative Study of the Metabolic and Skeletal Response of C57BL/6J and C57BL/6N Mice in a Diet-Induced Model of Type 2 Diabetes |
| title_full_unstemmed | A Comparative Study of the Metabolic and Skeletal Response of C57BL/6J and C57BL/6N Mice in a Diet-Induced Model of Type 2 Diabetes |
| title_short | A Comparative Study of the Metabolic and Skeletal Response of C57BL/6J and C57BL/6N Mice in a Diet-Induced Model of Type 2 Diabetes |
| title_sort | comparative study of the metabolic and skeletal response of c57bl 6j and c57bl 6n mice in a diet induced model of type 2 diabetes |
| url | http://dx.doi.org/10.1155/2015/758080 |
| work_keys_str_mv | AT elizabethrendinaruedy acomparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT kelseydhembree acomparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT angelasasaki acomparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT mckalerdavis acomparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT stanalightfoot acomparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT stephenlclarke acomparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT edralinalucas acomparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT brendajsmith acomparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT elizabethrendinaruedy comparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT kelseydhembree comparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT angelasasaki comparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT mckalerdavis comparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT stanalightfoot comparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT stephenlclarke comparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT edralinalucas comparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes AT brendajsmith comparativestudyofthemetabolicandskeletalresponseofc57bl6jandc57bl6nmiceinadietinducedmodeloftype2diabetes |