Comprehensive toxicity assessment of silver nanoparticles on Bacteria, human vein endothelial cells, and Caenorhabditis Elegans
Silver nanoparticles (AgNPs) exhibit concentration-dependent toxicity across multiple biological systems. In this study, it was investigated the effects of AgNPs on bacteria (Escherichia coli and Bacillus subtilis), a model organism (Caenorhabditis elegans), and human vein endothelial cells (HUVECs)...
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| Language: | English |
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Elsevier
2025-03-01
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| Series: | Results in Chemistry |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S221171562500075X |
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| author | Bircan Dinç |
| author_facet | Bircan Dinç |
| author_sort | Bircan Dinç |
| collection | DOAJ |
| description | Silver nanoparticles (AgNPs) exhibit concentration-dependent toxicity across multiple biological systems. In this study, it was investigated the effects of AgNPs on bacteria (Escherichia coli and Bacillus subtilis), a model organism (Caenorhabditis elegans), and human vein endothelial cells (HUVECs) at low concentrations (10, 30, and 50 μg/mL).The AgNPs were synthesized using a chemical reduction method and thoroughly characterized, showing a hydrodynamic size of 55 nm, a zeta potential of −57 mV, and thermal decomposition temperatures ranging from 182 to 318 °C. Our results revealed a significant inhibition of bacterial growth (52 % at 50 μg/mL), a 25 % reduction in C. elegans reproduction at just 10 μg/mL, and a decrease in body bending frequency from 42 to 19 beats per minute. In HUVEC cells, the IC50 was found to be 38 μg/mL, with cellular uptake increasing proportionally to concentration after 48 h.This study highlights the unique toxicological profile of AgNPs at low concentrations across multiple biological systems, emphasizing the importance of careful consideration for their safe use in biomedical and environmental applications. |
| format | Article |
| id | doaj-art-d47912338e56412183ef08da85098bcf |
| institution | Kabale University |
| issn | 2211-7156 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Results in Chemistry |
| spelling | doaj-art-d47912338e56412183ef08da85098bcf2025-02-06T05:11:34ZengElsevierResults in Chemistry2211-71562025-03-0114102092Comprehensive toxicity assessment of silver nanoparticles on Bacteria, human vein endothelial cells, and Caenorhabditis ElegansBircan Dinç0Biophysics, School of Medicine, Bahcesehir University, Sahrayı Cedit, Batman Sk. No:66, 34734, Kadıköy, İstanbul, TurkeySilver nanoparticles (AgNPs) exhibit concentration-dependent toxicity across multiple biological systems. In this study, it was investigated the effects of AgNPs on bacteria (Escherichia coli and Bacillus subtilis), a model organism (Caenorhabditis elegans), and human vein endothelial cells (HUVECs) at low concentrations (10, 30, and 50 μg/mL).The AgNPs were synthesized using a chemical reduction method and thoroughly characterized, showing a hydrodynamic size of 55 nm, a zeta potential of −57 mV, and thermal decomposition temperatures ranging from 182 to 318 °C. Our results revealed a significant inhibition of bacterial growth (52 % at 50 μg/mL), a 25 % reduction in C. elegans reproduction at just 10 μg/mL, and a decrease in body bending frequency from 42 to 19 beats per minute. In HUVEC cells, the IC50 was found to be 38 μg/mL, with cellular uptake increasing proportionally to concentration after 48 h.This study highlights the unique toxicological profile of AgNPs at low concentrations across multiple biological systems, emphasizing the importance of careful consideration for their safe use in biomedical and environmental applications.http://www.sciencedirect.com/science/article/pii/S221171562500075XSilver nanoparticlesCharacterizationAntibacterial propertiesToxicityCaenorhabditis elegans |
| spellingShingle | Bircan Dinç Comprehensive toxicity assessment of silver nanoparticles on Bacteria, human vein endothelial cells, and Caenorhabditis Elegans Results in Chemistry Silver nanoparticles Characterization Antibacterial properties Toxicity Caenorhabditis elegans |
| title | Comprehensive toxicity assessment of silver nanoparticles on Bacteria, human vein endothelial cells, and Caenorhabditis Elegans |
| title_full | Comprehensive toxicity assessment of silver nanoparticles on Bacteria, human vein endothelial cells, and Caenorhabditis Elegans |
| title_fullStr | Comprehensive toxicity assessment of silver nanoparticles on Bacteria, human vein endothelial cells, and Caenorhabditis Elegans |
| title_full_unstemmed | Comprehensive toxicity assessment of silver nanoparticles on Bacteria, human vein endothelial cells, and Caenorhabditis Elegans |
| title_short | Comprehensive toxicity assessment of silver nanoparticles on Bacteria, human vein endothelial cells, and Caenorhabditis Elegans |
| title_sort | comprehensive toxicity assessment of silver nanoparticles on bacteria human vein endothelial cells and caenorhabditis elegans |
| topic | Silver nanoparticles Characterization Antibacterial properties Toxicity Caenorhabditis elegans |
| url | http://www.sciencedirect.com/science/article/pii/S221171562500075X |
| work_keys_str_mv | AT bircandinc comprehensivetoxicityassessmentofsilvernanoparticlesonbacteriahumanveinendothelialcellsandcaenorhabditiselegans |