Lipidomic Signature of Pregnant and Postpartum Females by Longitudinal and Transversal Evaluation: Putative Biomarkers Determined by UHPLC-QTOF-ESI<sup>+</sup>-MS
Background: Pregnancy induces significant physiological and metabolic changes in the mother to support fetal growth and prepare for childbirth. These adaptations impact various systems, including immune tolerance, metabolism, and endocrine function. While metabolomics has been utilized to study preg...
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2025-01-01
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| author | Alexandra Traila Marius Craina Carmen Socaciu Andreea Iulia Socaciu Diana Nitusca Catalin Marian |
| author_facet | Alexandra Traila Marius Craina Carmen Socaciu Andreea Iulia Socaciu Diana Nitusca Catalin Marian |
| author_sort | Alexandra Traila |
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| description | Background: Pregnancy induces significant physiological and metabolic changes in the mother to support fetal growth and prepare for childbirth. These adaptations impact various systems, including immune tolerance, metabolism, and endocrine function. While metabolomics has been utilized to study pregnancy-related metabolic changes, comprehensive comparisons between pregnant and non-pregnant states, particularly using ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS), remain limited. Methods: This study aimed to explore the dynamic, longitudinal metabolic shifts during pregnancy by profiling plasma samples from 65 pregnant women across three time points (6–14 weeks, 14–22 weeks, and >24 weeks) and 42 postpartum women. Lipidomics was prioritized, and a solvent mixture was employed to enhance lipid extraction, using UHPLC-QTOF-ESI<sup>+</sup>-MS. Results: A total of 290 metabolites were identified and analyzed. Our results revealed significant metabolic differences between pregnant and postpartum women, with lipid molecules such as estrogen derivatives, fatty acids, and ceramides showing strong potential as biomarkers. Further biomarker analysis highlighted distinct metabolic signatures between early and late pregnancy stages, particularly in lipid metabolism (with AUC values > 0.8). Conclusions: These findings contribute to a deeper understanding of pregnancy-related metabolic changes and may offer insights into maternal and neonatal health outcomes. |
| format | Article |
| id | doaj-art-d44ff0991e2c459db72287ca2c851001 |
| institution | Kabale University |
| issn | 2218-1989 |
| language | English |
| publishDate | 2025-01-01 |
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| series | Metabolites |
| spelling | doaj-art-d44ff0991e2c459db72287ca2c8510012025-01-24T13:41:13ZengMDPI AGMetabolites2218-19892025-01-011512710.3390/metabo15010027Lipidomic Signature of Pregnant and Postpartum Females by Longitudinal and Transversal Evaluation: Putative Biomarkers Determined by UHPLC-QTOF-ESI<sup>+</sup>-MSAlexandra Traila0Marius Craina1Carmen Socaciu2Andreea Iulia Socaciu3Diana Nitusca4Catalin Marian5Doctoral School, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, RomaniaDepartment of Obstetrics and Gynecology, “Victor Babes” University of Medicine and Pharmacy Timisoara, Eftimie Murgu Square 2, 300041 Timisoara, RomaniaBiodiatech, Research Center for Applied Biotechnology in Diagnosis and Molecular Therapy, 400478 Cluj-Napoca, RomaniaDepartment of Occupational Health, Iuliu Hateganu University of Medicine and Pharmacy, Str. Victor Babes Nr. 8, 400347 Cluj-Napoca, RomaniaDepartment of Biochemistry and Pharmacology, Victor Babes University of Medicine and Pharmacy, Pta Eftimie Murgu Nr. 2, 300041 Timisoara, RomaniaDepartment of Biochemistry and Pharmacology, Victor Babes University of Medicine and Pharmacy, Pta Eftimie Murgu Nr. 2, 300041 Timisoara, RomaniaBackground: Pregnancy induces significant physiological and metabolic changes in the mother to support fetal growth and prepare for childbirth. These adaptations impact various systems, including immune tolerance, metabolism, and endocrine function. While metabolomics has been utilized to study pregnancy-related metabolic changes, comprehensive comparisons between pregnant and non-pregnant states, particularly using ultra-high-performance liquid chromatography coupled with mass spectrometry (UHPLC-MS), remain limited. Methods: This study aimed to explore the dynamic, longitudinal metabolic shifts during pregnancy by profiling plasma samples from 65 pregnant women across three time points (6–14 weeks, 14–22 weeks, and >24 weeks) and 42 postpartum women. Lipidomics was prioritized, and a solvent mixture was employed to enhance lipid extraction, using UHPLC-QTOF-ESI<sup>+</sup>-MS. Results: A total of 290 metabolites were identified and analyzed. Our results revealed significant metabolic differences between pregnant and postpartum women, with lipid molecules such as estrogen derivatives, fatty acids, and ceramides showing strong potential as biomarkers. Further biomarker analysis highlighted distinct metabolic signatures between early and late pregnancy stages, particularly in lipid metabolism (with AUC values > 0.8). Conclusions: These findings contribute to a deeper understanding of pregnancy-related metabolic changes and may offer insights into maternal and neonatal health outcomes.https://www.mdpi.com/2218-1989/15/1/27lipidomicsbiomarkersmetabolomicspregnancypostpartum |
| spellingShingle | Alexandra Traila Marius Craina Carmen Socaciu Andreea Iulia Socaciu Diana Nitusca Catalin Marian Lipidomic Signature of Pregnant and Postpartum Females by Longitudinal and Transversal Evaluation: Putative Biomarkers Determined by UHPLC-QTOF-ESI<sup>+</sup>-MS Metabolites lipidomics biomarkers metabolomics pregnancy postpartum |
| title | Lipidomic Signature of Pregnant and Postpartum Females by Longitudinal and Transversal Evaluation: Putative Biomarkers Determined by UHPLC-QTOF-ESI<sup>+</sup>-MS |
| title_full | Lipidomic Signature of Pregnant and Postpartum Females by Longitudinal and Transversal Evaluation: Putative Biomarkers Determined by UHPLC-QTOF-ESI<sup>+</sup>-MS |
| title_fullStr | Lipidomic Signature of Pregnant and Postpartum Females by Longitudinal and Transversal Evaluation: Putative Biomarkers Determined by UHPLC-QTOF-ESI<sup>+</sup>-MS |
| title_full_unstemmed | Lipidomic Signature of Pregnant and Postpartum Females by Longitudinal and Transversal Evaluation: Putative Biomarkers Determined by UHPLC-QTOF-ESI<sup>+</sup>-MS |
| title_short | Lipidomic Signature of Pregnant and Postpartum Females by Longitudinal and Transversal Evaluation: Putative Biomarkers Determined by UHPLC-QTOF-ESI<sup>+</sup>-MS |
| title_sort | lipidomic signature of pregnant and postpartum females by longitudinal and transversal evaluation putative biomarkers determined by uhplc qtof esi sup sup ms |
| topic | lipidomics biomarkers metabolomics pregnancy postpartum |
| url | https://www.mdpi.com/2218-1989/15/1/27 |
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