A Simple Microfluidic Chip Design for Fundamental Bioseparation
A microchip pressure-driven liquid chromatographic system with a packed column has been designed and fabricated by using poly(dimethylsiloxane) (PDMS). The liquid chromatographic column was packed with mesoporous silica beads of Ia3d space group. Separation of dyes and biopolymers was carried out to...
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| Format: | Article |
| Language: | English |
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Wiley
2014-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2014/175457 |
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| author | Alan S. Chan Michael K. Danquah Dominic Agyei Patrick G. Hartley Yonggang Zhu |
| author_facet | Alan S. Chan Michael K. Danquah Dominic Agyei Patrick G. Hartley Yonggang Zhu |
| author_sort | Alan S. Chan |
| collection | DOAJ |
| description | A microchip pressure-driven liquid chromatographic system with a packed column has been designed and fabricated by using poly(dimethylsiloxane) (PDMS). The liquid chromatographic column was packed with mesoporous silica beads of Ia3d space group. Separation of dyes and biopolymers was carried out to verify the performance of the chip. A mixture of dyes (fluorescein and rhodamine B) and a biopolymer mixture (10 kDa Dextran and 66 kDa BSA) were separated and the fluorescence technique was employed to detect the movement of the molecules. Fluorescein molecule was a nonretained species and rhodamine B was attached onto silica surface when dye mixture in deionized water was injected into the microchannel. The retention times for dextran molecule and BSA molecule in biopolymer separation experiment were 45 s and 120 s, respectively. Retention factor was estimated to be 3.3 for dextran and 10.4 for BSA. The selectivity was 3.2 and resolution was 10.7. Good separation of dyes and biopolymers was achieved and the chip design was verified. |
| format | Article |
| id | doaj-art-d4309cf05cd74f8fa1102978e9c70935 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-d4309cf05cd74f8fa1102978e9c709352025-02-03T01:22:00ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732014-01-01201410.1155/2014/175457175457A Simple Microfluidic Chip Design for Fundamental BioseparationAlan S. Chan0Michael K. Danquah1Dominic Agyei2Patrick G. Hartley3Yonggang Zhu4CSIRO Materials Science and Engineering, Highett, VIC 3190, AustraliaDepartment of Chemical Engineering, Monash University, Clayton, VIC 3800, AustraliaDepartment of Chemical Engineering, Monash University, Clayton, VIC 3800, AustraliaCSIRO Materials Science and Engineering, Highett, VIC 3190, AustraliaCSIRO Materials Science and Engineering, Highett, VIC 3190, AustraliaA microchip pressure-driven liquid chromatographic system with a packed column has been designed and fabricated by using poly(dimethylsiloxane) (PDMS). The liquid chromatographic column was packed with mesoporous silica beads of Ia3d space group. Separation of dyes and biopolymers was carried out to verify the performance of the chip. A mixture of dyes (fluorescein and rhodamine B) and a biopolymer mixture (10 kDa Dextran and 66 kDa BSA) were separated and the fluorescence technique was employed to detect the movement of the molecules. Fluorescein molecule was a nonretained species and rhodamine B was attached onto silica surface when dye mixture in deionized water was injected into the microchannel. The retention times for dextran molecule and BSA molecule in biopolymer separation experiment were 45 s and 120 s, respectively. Retention factor was estimated to be 3.3 for dextran and 10.4 for BSA. The selectivity was 3.2 and resolution was 10.7. Good separation of dyes and biopolymers was achieved and the chip design was verified.http://dx.doi.org/10.1155/2014/175457 |
| spellingShingle | Alan S. Chan Michael K. Danquah Dominic Agyei Patrick G. Hartley Yonggang Zhu A Simple Microfluidic Chip Design for Fundamental Bioseparation Journal of Analytical Methods in Chemistry |
| title | A Simple Microfluidic Chip Design for Fundamental Bioseparation |
| title_full | A Simple Microfluidic Chip Design for Fundamental Bioseparation |
| title_fullStr | A Simple Microfluidic Chip Design for Fundamental Bioseparation |
| title_full_unstemmed | A Simple Microfluidic Chip Design for Fundamental Bioseparation |
| title_short | A Simple Microfluidic Chip Design for Fundamental Bioseparation |
| title_sort | simple microfluidic chip design for fundamental bioseparation |
| url | http://dx.doi.org/10.1155/2014/175457 |
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