From Cartilage to Matrix: Protocols for the Decellularization of Porcine Auricular Cartilage
The shortage of tissues and damaged organs led to the development of tissue engineering. Biological scaffolds, created from the extracellular matrix (ECM) of organs and tissues, have emerged as a promising solution for transplants. The ECM of decellularized auricular cartilage is a potential tool fo...
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MDPI AG
2025-01-01
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| author | Ana Caroline dos Santos Livia Maria Barbosa de Andrade Raí André Querino Candelária Juliana Casanovas de Carvalho Maria Carolina Miglino Valbão Rodrigo da Silva Nunes Barreto Marcelo Domingues de Faria Rogerio Leone Buchaim Daniela Vieira Buchaim Maria Angelica Miglino |
| author_facet | Ana Caroline dos Santos Livia Maria Barbosa de Andrade Raí André Querino Candelária Juliana Casanovas de Carvalho Maria Carolina Miglino Valbão Rodrigo da Silva Nunes Barreto Marcelo Domingues de Faria Rogerio Leone Buchaim Daniela Vieira Buchaim Maria Angelica Miglino |
| author_sort | Ana Caroline dos Santos |
| collection | DOAJ |
| description | The shortage of tissues and damaged organs led to the development of tissue engineering. Biological scaffolds, created from the extracellular matrix (ECM) of organs and tissues, have emerged as a promising solution for transplants. The ECM of decellularized auricular cartilage is a potential tool for producing ideal scaffolds for the recellularization and implantation of new tissue in damaged areas. In order to be classified as an ideal scaffold, it must be acellular, preserving its proteins and physical characteristics necessary for cell adhesion. This study aimed to develop a decellularization protocol for pig ear cartilage and evaluate the integrity of the ECM. Four tests were performed using different methods and protocols, with four pig ears from which the skin and subcutaneous tissue were removed, leaving only the cartilage. The most efficient protocol was the combination of trypsin with a sodium hydroxide solution (0.2 N) and SDS (1%) without altering the ECM conformation or the collagen architecture. In conclusion, it was observed that auricular cartilage is difficult to decellularize, influenced by material size, exposure time, and the composition of the solution. Freezing and thawing did not affect the procedure. The sample thickness significantly impacted the decellularization time. |
| format | Article |
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| institution | Kabale University |
| issn | 2306-5354 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Bioengineering |
| spelling | doaj-art-d4129673c49341c997ec1c1ba0e2b26e2025-01-24T13:23:06ZengMDPI AGBioengineering2306-53542025-01-011215210.3390/bioengineering12010052From Cartilage to Matrix: Protocols for the Decellularization of Porcine Auricular CartilageAna Caroline dos Santos0Livia Maria Barbosa de Andrade1Raí André Querino Candelária2Juliana Casanovas de Carvalho3Maria Carolina Miglino Valbão4Rodrigo da Silva Nunes Barreto5Marcelo Domingues de Faria6Rogerio Leone Buchaim7Daniela Vieira Buchaim8Maria Angelica Miglino9Graduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), São Paulo 05508-270, BrazilGraduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), São Paulo 05508-270, BrazilGraduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), São Paulo 05508-270, BrazilGraduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), São Paulo 05508-270, BrazilMedical School, University of Marília (UNIMAR), Marília 17525-902, BrazilGraduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), São Paulo 05508-270, BrazilDepartment of Animal Anatomy, Agricultural Sciences—Federal University of Vale do São Francisco (UNIVASF), Petrolina 56300-000, BrazilGraduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), São Paulo 05508-270, BrazilGraduate Program in Anatomy of Domestic and Wild Animals, Faculty of Veterinary Medicine and Animal Science, University of São Paulo (FMVZ/USP), São Paulo 05508-270, BrazilPostgraduate Program in Structural and Functional Interactions in Rehabilitation, Postgraduate Department, University of Marilia (UNIMAR), Marilia 17525-902, BrazilThe shortage of tissues and damaged organs led to the development of tissue engineering. Biological scaffolds, created from the extracellular matrix (ECM) of organs and tissues, have emerged as a promising solution for transplants. The ECM of decellularized auricular cartilage is a potential tool for producing ideal scaffolds for the recellularization and implantation of new tissue in damaged areas. In order to be classified as an ideal scaffold, it must be acellular, preserving its proteins and physical characteristics necessary for cell adhesion. This study aimed to develop a decellularization protocol for pig ear cartilage and evaluate the integrity of the ECM. Four tests were performed using different methods and protocols, with four pig ears from which the skin and subcutaneous tissue were removed, leaving only the cartilage. The most efficient protocol was the combination of trypsin with a sodium hydroxide solution (0.2 N) and SDS (1%) without altering the ECM conformation or the collagen architecture. In conclusion, it was observed that auricular cartilage is difficult to decellularize, influenced by material size, exposure time, and the composition of the solution. Freezing and thawing did not affect the procedure. The sample thickness significantly impacted the decellularization time.https://www.mdpi.com/2306-5354/12/1/52tissue engineeringrecellularizationbiological scaffoldsextracellular matrixcartilaginous tissueenzymatic decellularization |
| spellingShingle | Ana Caroline dos Santos Livia Maria Barbosa de Andrade Raí André Querino Candelária Juliana Casanovas de Carvalho Maria Carolina Miglino Valbão Rodrigo da Silva Nunes Barreto Marcelo Domingues de Faria Rogerio Leone Buchaim Daniela Vieira Buchaim Maria Angelica Miglino From Cartilage to Matrix: Protocols for the Decellularization of Porcine Auricular Cartilage Bioengineering tissue engineering recellularization biological scaffolds extracellular matrix cartilaginous tissue enzymatic decellularization |
| title | From Cartilage to Matrix: Protocols for the Decellularization of Porcine Auricular Cartilage |
| title_full | From Cartilage to Matrix: Protocols for the Decellularization of Porcine Auricular Cartilage |
| title_fullStr | From Cartilage to Matrix: Protocols for the Decellularization of Porcine Auricular Cartilage |
| title_full_unstemmed | From Cartilage to Matrix: Protocols for the Decellularization of Porcine Auricular Cartilage |
| title_short | From Cartilage to Matrix: Protocols for the Decellularization of Porcine Auricular Cartilage |
| title_sort | from cartilage to matrix protocols for the decellularization of porcine auricular cartilage |
| topic | tissue engineering recellularization biological scaffolds extracellular matrix cartilaginous tissue enzymatic decellularization |
| url | https://www.mdpi.com/2306-5354/12/1/52 |
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