From Cartilage to Matrix: Protocols for the Decellularization of Porcine Auricular Cartilage

From Cartilage to Matrix: Protocols for the Decellularization of Porcine Auricular Cartilage

The shortage of tissues and damaged organs led to the development of tissue engineering. Biological scaffolds, created from the extracellular matrix (ECM) of organs and tissues, have emerged as a promising solution for transplants. The ECM of decellularized auricular cartilage is a potential tool fo...

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Main Authors: Ana Caroline dos Santos, Livia Maria Barbosa de Andrade, Raí André Querino Candelária, Juliana Casanovas de Carvalho, Maria Carolina Miglino Valbão, Rodrigo da Silva Nunes Barreto, Marcelo Domingues de Faria, Rogerio Leone Buchaim, Daniela Vieira Buchaim, Maria Angelica Miglino
Format: Article
Language:English
Published: MDPI AG 2025-01-01
Series:Bioengineering
Subjects:
tissue engineering
recellularization
biological scaffolds
extracellular matrix
cartilaginous tissue
enzymatic decellularization
Online Access:https://www.mdpi.com/2306-5354/12/1/52
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Summary:The shortage of tissues and damaged organs led to the development of tissue engineering. Biological scaffolds, created from the extracellular matrix (ECM) of organs and tissues, have emerged as a promising solution for transplants. The ECM of decellularized auricular cartilage is a potential tool for producing ideal scaffolds for the recellularization and implantation of new tissue in damaged areas. In order to be classified as an ideal scaffold, it must be acellular, preserving its proteins and physical characteristics necessary for cell adhesion. This study aimed to develop a decellularization protocol for pig ear cartilage and evaluate the integrity of the ECM. Four tests were performed using different methods and protocols, with four pig ears from which the skin and subcutaneous tissue were removed, leaving only the cartilage. The most efficient protocol was the combination of trypsin with a sodium hydroxide solution (0.2 N) and SDS (1%) without altering the ECM conformation or the collagen architecture. In conclusion, it was observed that auricular cartilage is difficult to decellularize, influenced by material size, exposure time, and the composition of the solution. Freezing and thawing did not affect the procedure. The sample thickness significantly impacted the decellularization time.
ISSN:2306-5354

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