Molecular Identification of Enterocytozoon bieneusi Isolates from Nigerian Children
A study was conducted to detect and identify enteric microsporidian species in 43 children from Oyo state, Nigeria. Using nested polymerase chain reaction, 9.3% of the children were identified as positive for Enterocytozoon bieneusi. DNA sequencing of the PCR products showed the presence of three kn...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Journal of Parasitology Research |
| Online Access: | http://dx.doi.org/10.1155/2011/129542 |
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| _version_ | 1832567622457098240 |
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| author | Adekunle Bamidele Ayinmode Oladele Teslim Ojuromi Lihua Xiao |
| author_facet | Adekunle Bamidele Ayinmode Oladele Teslim Ojuromi Lihua Xiao |
| author_sort | Adekunle Bamidele Ayinmode |
| collection | DOAJ |
| description | A study was conducted to detect and identify enteric microsporidian species in 43 children from Oyo state, Nigeria. Using nested polymerase chain reaction, 9.3% of the children were identified as positive for Enterocytozoon bieneusi. DNA sequencing of the PCR products showed the presence of three known genotypes (two isolates of genotype D and one of genotype K) and one new genotype. This study suggests that either human or animal (or both) could be the infection source for the children, since identified genotypes D and K have been previously detected in both immunocompromised and immunocompetent patients and domestic animals. The identification of high diversity also suggests intensive transmission of microsporidiosis in the studied area. |
| format | Article |
| id | doaj-art-d3e3b9ca77974daca8ece075fdb15cb9 |
| institution | Kabale University |
| issn | 2090-0023 2090-0031 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Parasitology Research |
| spelling | doaj-art-d3e3b9ca77974daca8ece075fdb15cb92025-02-03T01:01:04ZengWileyJournal of Parasitology Research2090-00232090-00312011-01-01201110.1155/2011/129542129542Molecular Identification of Enterocytozoon bieneusi Isolates from Nigerian ChildrenAdekunle Bamidele Ayinmode0Oladele Teslim Ojuromi1Lihua Xiao2Department of Veterinary Microbiology and Parasitology, Faculty of Veterinary Medicine, University of Ibadan, Ibadan, NigeriaDepartment of Zoology, Lagos State University, Ojo, Lagos, NigeriaDivision of Foodborne, Waterborne, and Environmental Diseases, Centers for Disease Control and Prevention, Atlanta, GA 30333, USAA study was conducted to detect and identify enteric microsporidian species in 43 children from Oyo state, Nigeria. Using nested polymerase chain reaction, 9.3% of the children were identified as positive for Enterocytozoon bieneusi. DNA sequencing of the PCR products showed the presence of three known genotypes (two isolates of genotype D and one of genotype K) and one new genotype. This study suggests that either human or animal (or both) could be the infection source for the children, since identified genotypes D and K have been previously detected in both immunocompromised and immunocompetent patients and domestic animals. The identification of high diversity also suggests intensive transmission of microsporidiosis in the studied area.http://dx.doi.org/10.1155/2011/129542 |
| spellingShingle | Adekunle Bamidele Ayinmode Oladele Teslim Ojuromi Lihua Xiao Molecular Identification of Enterocytozoon bieneusi Isolates from Nigerian Children Journal of Parasitology Research |
| title | Molecular Identification of Enterocytozoon bieneusi Isolates from Nigerian Children |
| title_full | Molecular Identification of Enterocytozoon bieneusi Isolates from Nigerian Children |
| title_fullStr | Molecular Identification of Enterocytozoon bieneusi Isolates from Nigerian Children |
| title_full_unstemmed | Molecular Identification of Enterocytozoon bieneusi Isolates from Nigerian Children |
| title_short | Molecular Identification of Enterocytozoon bieneusi Isolates from Nigerian Children |
| title_sort | molecular identification of enterocytozoon bieneusi isolates from nigerian children |
| url | http://dx.doi.org/10.1155/2011/129542 |
| work_keys_str_mv | AT adekunlebamideleayinmode molecularidentificationofenterocytozoonbieneusiisolatesfromnigerianchildren AT oladeleteslimojuromi molecularidentificationofenterocytozoonbieneusiisolatesfromnigerianchildren AT lihuaxiao molecularidentificationofenterocytozoonbieneusiisolatesfromnigerianchildren |