Molecular Identification of Enterocytozoon bieneusi Isolates from Nigerian Children
A study was conducted to detect and identify enteric microsporidian species in 43 children from Oyo state, Nigeria. Using nested polymerase chain reaction, 9.3% of the children were identified as positive for Enterocytozoon bieneusi. DNA sequencing of the PCR products showed the presence of three kn...
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Main Authors: | , , |
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Format: | Article |
Language: | English |
Published: |
Wiley
2011-01-01
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Series: | Journal of Parasitology Research |
Online Access: | http://dx.doi.org/10.1155/2011/129542 |
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Summary: | A study was conducted to detect and identify enteric microsporidian species in 43 children from Oyo state, Nigeria. Using nested polymerase chain reaction, 9.3% of the children were identified as positive for Enterocytozoon bieneusi. DNA sequencing of the PCR products showed the presence of three known genotypes (two isolates of genotype D and one of genotype K) and one new genotype. This study suggests that either human or animal (or both) could be the infection source for the children, since identified genotypes D and K have been previously detected in both immunocompromised and immunocompetent patients and domestic animals. The identification of high diversity also suggests intensive transmission of microsporidiosis in the studied area. |
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ISSN: | 2090-0023 2090-0031 |