<i>Musa paradisiaca</i> L. Inflorescence Abrogates Neutrophil Activation by Downregulating TLR4/NF-KB Signaling Pathway in LPS-Induced Acute Lung Injury Model
<b>Background/Objectives</b>: Acute lung injury (ALI) is an inflammatory disorder affecting patients in intensive care with high mortality. No specific pharmacological treatment is available. <i>Musa paradisiaca</i> L. (banana) is a cosmopolitan plant, and homemade syrup from...
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MDPI AG
2024-12-01
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| Online Access: | https://www.mdpi.com/1424-8247/18/1/8 |
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| author | Francisco Allysson Assis Ferreira Gadelha Raquel Fragoso Pereira Cavalcanti Cosmo Isaias Duvirgens Vieira Joao Batista De Oliveira Louíse Mangueira De Lima Adriano Francisco Alves Matheus Marley Bezerra Pessoa Leônia Maria Batista Naiara Naiana Dejani Marcia Regina Piuvezam |
| author_facet | Francisco Allysson Assis Ferreira Gadelha Raquel Fragoso Pereira Cavalcanti Cosmo Isaias Duvirgens Vieira Joao Batista De Oliveira Louíse Mangueira De Lima Adriano Francisco Alves Matheus Marley Bezerra Pessoa Leônia Maria Batista Naiara Naiana Dejani Marcia Regina Piuvezam |
| author_sort | Francisco Allysson Assis Ferreira Gadelha |
| collection | DOAJ |
| description | <b>Background/Objectives</b>: Acute lung injury (ALI) is an inflammatory disorder affecting patients in intensive care with high mortality. No specific pharmacological treatment is available. <i>Musa paradisiaca</i> L. (banana) is a cosmopolitan plant, and homemade syrup from its inflorescence is used in many countries to treat pulmonary inflammation. Therefore, this study analyzed the hydroalcoholic extract (HEM) of the inflorescence on the ALI experimental model. <b>Methods</b>: Swiss mice were challenged with lipopolysaccharide and treated with HEM after 1, 24, and 48 h (five animals/group, three times). <b>Results</b>: The HEM-treated ALI mice presented a decrease in neutrophil migration in the bronchoalveolar lavage fluid (BALF), in the alveolar region, and in the blood, correlating to downregulation of CD18 expression. The HEM treatment also reduced the protein concentration in the BALF, caused lung edema formation, impaired NF-κB activation via inhibition of TLR4 signaling pathway, and decreased IL-1β, TNF-α production, free DNA release, and myeloperoxidase (MPO) activity. However, the extract induced an increased IL-10 in the BALF. <b>Conclusions</b>: Therefore, HEM’s anti-inflammatory and immunomodulatory activities in ALI mice are by deactivating neutrophils by decreasing CD18 receptor, free DNA release, and MPO activity and inducing IL-10 production. Thus, this study supports the use of banana inflorescence in folk medicine and suggests its rational use to develop a phytomedicine to treat pulmonary inflammation. |
| format | Article |
| id | doaj-art-d3a4d5e86e384acbb5ad2e8b637fb45c |
| institution | Kabale University |
| issn | 1424-8247 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceuticals |
| spelling | doaj-art-d3a4d5e86e384acbb5ad2e8b637fb45c2025-01-24T13:44:58ZengMDPI AGPharmaceuticals1424-82472024-12-01181810.3390/ph18010008<i>Musa paradisiaca</i> L. Inflorescence Abrogates Neutrophil Activation by Downregulating TLR4/NF-KB Signaling Pathway in LPS-Induced Acute Lung Injury ModelFrancisco Allysson Assis Ferreira Gadelha0Raquel Fragoso Pereira Cavalcanti1Cosmo Isaias Duvirgens Vieira2Joao Batista De Oliveira3Louíse Mangueira De Lima4Adriano Francisco Alves5Matheus Marley Bezerra Pessoa6Leônia Maria Batista7Naiara Naiana Dejani8Marcia Regina Piuvezam9Laboratory of Immunopharmacology, Research Institute for Drugs and Medicines, Federal University of Paraíba, João Pessoa 58051-900, PB, BrazilPostgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Paraíba, João Pessoa 58051-900, PB, BrazilPostgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Paraíba, João Pessoa 58051-900, PB, BrazilPostgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Paraíba, João Pessoa 58051-900, PB, BrazilPostgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Paraíba, João Pessoa 58051-900, PB, BrazilDepartment of Phisiology and Phatology, Health Sciences Center, Federal University of Paraíba, João Pessoa 58051-900, PB, BrazilPostgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Paraíba, João Pessoa 58051-900, PB, BrazilPostgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Paraíba, João Pessoa 58051-900, PB, BrazilDepartment of Phisiology and Phatology, Health Sciences Center, Federal University of Paraíba, João Pessoa 58051-900, PB, BrazilPostgraduate Program in Natural and Synthetic Bioactive Products, Federal University of Paraíba, João Pessoa 58051-900, PB, Brazil<b>Background/Objectives</b>: Acute lung injury (ALI) is an inflammatory disorder affecting patients in intensive care with high mortality. No specific pharmacological treatment is available. <i>Musa paradisiaca</i> L. (banana) is a cosmopolitan plant, and homemade syrup from its inflorescence is used in many countries to treat pulmonary inflammation. Therefore, this study analyzed the hydroalcoholic extract (HEM) of the inflorescence on the ALI experimental model. <b>Methods</b>: Swiss mice were challenged with lipopolysaccharide and treated with HEM after 1, 24, and 48 h (five animals/group, three times). <b>Results</b>: The HEM-treated ALI mice presented a decrease in neutrophil migration in the bronchoalveolar lavage fluid (BALF), in the alveolar region, and in the blood, correlating to downregulation of CD18 expression. The HEM treatment also reduced the protein concentration in the BALF, caused lung edema formation, impaired NF-κB activation via inhibition of TLR4 signaling pathway, and decreased IL-1β, TNF-α production, free DNA release, and myeloperoxidase (MPO) activity. However, the extract induced an increased IL-10 in the BALF. <b>Conclusions</b>: Therefore, HEM’s anti-inflammatory and immunomodulatory activities in ALI mice are by deactivating neutrophils by decreasing CD18 receptor, free DNA release, and MPO activity and inducing IL-10 production. Thus, this study supports the use of banana inflorescence in folk medicine and suggests its rational use to develop a phytomedicine to treat pulmonary inflammation.https://www.mdpi.com/1424-8247/18/1/8banana heartimmunomodulatory activityDNAmyeloperoxidase |
| spellingShingle | Francisco Allysson Assis Ferreira Gadelha Raquel Fragoso Pereira Cavalcanti Cosmo Isaias Duvirgens Vieira Joao Batista De Oliveira Louíse Mangueira De Lima Adriano Francisco Alves Matheus Marley Bezerra Pessoa Leônia Maria Batista Naiara Naiana Dejani Marcia Regina Piuvezam <i>Musa paradisiaca</i> L. Inflorescence Abrogates Neutrophil Activation by Downregulating TLR4/NF-KB Signaling Pathway in LPS-Induced Acute Lung Injury Model Pharmaceuticals banana heart immunomodulatory activity DNA myeloperoxidase |
| title | <i>Musa paradisiaca</i> L. Inflorescence Abrogates Neutrophil Activation by Downregulating TLR4/NF-KB Signaling Pathway in LPS-Induced Acute Lung Injury Model |
| title_full | <i>Musa paradisiaca</i> L. Inflorescence Abrogates Neutrophil Activation by Downregulating TLR4/NF-KB Signaling Pathway in LPS-Induced Acute Lung Injury Model |
| title_fullStr | <i>Musa paradisiaca</i> L. Inflorescence Abrogates Neutrophil Activation by Downregulating TLR4/NF-KB Signaling Pathway in LPS-Induced Acute Lung Injury Model |
| title_full_unstemmed | <i>Musa paradisiaca</i> L. Inflorescence Abrogates Neutrophil Activation by Downregulating TLR4/NF-KB Signaling Pathway in LPS-Induced Acute Lung Injury Model |
| title_short | <i>Musa paradisiaca</i> L. Inflorescence Abrogates Neutrophil Activation by Downregulating TLR4/NF-KB Signaling Pathway in LPS-Induced Acute Lung Injury Model |
| title_sort | i musa paradisiaca i l inflorescence abrogates neutrophil activation by downregulating tlr4 nf kb signaling pathway in lps induced acute lung injury model |
| topic | banana heart immunomodulatory activity DNA myeloperoxidase |
| url | https://www.mdpi.com/1424-8247/18/1/8 |
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