Rust HUBB: DNA barcode-based identification of Pucciniales
Abstract Rust fungi (Pucciniales, Basidiomycota) are a species-rich (ca. 8000 species), globally distributed order of obligate plant pathogens. Rust species are host-specific, and as a group they cause disease on many of our most economically and/or ecologically significant plants. As such, the abil...
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| Language: | English |
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BMC
2024-02-01
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| Series: | IMA Fungus |
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| Online Access: | https://doi.org/10.1186/s43008-023-00132-7 |
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| author | Patricia Kaishian Christopher R. K. Layug Mark Anderson Diane R. Berg M. Catherine Aime |
| author_facet | Patricia Kaishian Christopher R. K. Layug Mark Anderson Diane R. Berg M. Catherine Aime |
| author_sort | Patricia Kaishian |
| collection | DOAJ |
| description | Abstract Rust fungi (Pucciniales, Basidiomycota) are a species-rich (ca. 8000 species), globally distributed order of obligate plant pathogens. Rust species are host-specific, and as a group they cause disease on many of our most economically and/or ecologically significant plants. As such, the ability to accurately and rapidly identify these fungi is of particular interest to mycologists, botanists, agricultural scientists, farmers, quarantine officials, and associated stakeholders. However, the complexities of the rust life cycle, which may include production of up to five different spore types and alternation between two unrelated host species, have made standard identifications, especially of less-documented spore states or alternate hosts, extremely difficult. The Arthur Fungarium (PUR) at Purdue University is home to one of the most comprehensive collections of rust fungi in the world. Using material vouchered in PUR supplemented with fresh collections we generated DNA barcodes of the 28S ribosomal repeat from > 3700 rust fungal specimens. Barcoded material spans 120 genera and > 1100 species, most represented by several replicate sequences. Barcodes and associated metadata are hosted in a publicly accessible, BLAST searchable database called Rust HUBB (Herbarium-based Universal Barcode Blast) and will be continuously updated. |
| format | Article |
| id | doaj-art-d375833ef1a940dc93d92f4f4ec69e32 |
| institution | Kabale University |
| issn | 2210-6359 |
| language | English |
| publishDate | 2024-02-01 |
| publisher | BMC |
| record_format | Article |
| series | IMA Fungus |
| spelling | doaj-art-d375833ef1a940dc93d92f4f4ec69e322025-02-03T09:13:41ZengBMCIMA Fungus2210-63592024-02-011511610.1186/s43008-023-00132-7Rust HUBB: DNA barcode-based identification of PuccinialesPatricia Kaishian0Christopher R. K. Layug1Mark Anderson2Diane R. Berg3M. Catherine Aime4Department of Botany and Plant Pathology, Purdue UniversityDepartment of Botany and Plant Pathology, Purdue UniversityDepartment of Botany and Plant Pathology, Purdue UniversityDepartment of Botany and Plant Pathology, Purdue UniversityDepartment of Botany and Plant Pathology, Purdue UniversityAbstract Rust fungi (Pucciniales, Basidiomycota) are a species-rich (ca. 8000 species), globally distributed order of obligate plant pathogens. Rust species are host-specific, and as a group they cause disease on many of our most economically and/or ecologically significant plants. As such, the ability to accurately and rapidly identify these fungi is of particular interest to mycologists, botanists, agricultural scientists, farmers, quarantine officials, and associated stakeholders. However, the complexities of the rust life cycle, which may include production of up to five different spore types and alternation between two unrelated host species, have made standard identifications, especially of less-documented spore states or alternate hosts, extremely difficult. The Arthur Fungarium (PUR) at Purdue University is home to one of the most comprehensive collections of rust fungi in the world. Using material vouchered in PUR supplemented with fresh collections we generated DNA barcodes of the 28S ribosomal repeat from > 3700 rust fungal specimens. Barcoded material spans 120 genera and > 1100 species, most represented by several replicate sequences. Barcodes and associated metadata are hosted in a publicly accessible, BLAST searchable database called Rust HUBB (Herbarium-based Universal Barcode Blast) and will be continuously updated.https://doi.org/10.1186/s43008-023-00132-7PucciniaColeosporiumUromycesPhakopsoraRaveneliaUredinales |
| spellingShingle | Patricia Kaishian Christopher R. K. Layug Mark Anderson Diane R. Berg M. Catherine Aime Rust HUBB: DNA barcode-based identification of Pucciniales IMA Fungus Puccinia Coleosporium Uromyces Phakopsora Ravenelia Uredinales |
| title | Rust HUBB: DNA barcode-based identification of Pucciniales |
| title_full | Rust HUBB: DNA barcode-based identification of Pucciniales |
| title_fullStr | Rust HUBB: DNA barcode-based identification of Pucciniales |
| title_full_unstemmed | Rust HUBB: DNA barcode-based identification of Pucciniales |
| title_short | Rust HUBB: DNA barcode-based identification of Pucciniales |
| title_sort | rust hubb dna barcode based identification of pucciniales |
| topic | Puccinia Coleosporium Uromyces Phakopsora Ravenelia Uredinales |
| url | https://doi.org/10.1186/s43008-023-00132-7 |
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