PLLA honeycombs activated by plasma and high-energy excimer laser for stem cell support
In this study, we constructed and activated honeycomb structures on perfluorinated substrates subjected to KrF laser treatment (wavelength 248 nm). We selected the biopolymer poly L-lactic acid (PLLA) as the honeycomb material, which was dissolved in a mixture of chloroform/methanol. A micropattern...
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Elsevier
2025-01-01
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| Series: | Applied Surface Science Advances |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666523924000904 |
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| author | P. Slepička N. Slepičková Kasálková J. Musílková L. Bačáková B. Frýdlová P. Sajdl Z. Kolská E. Rebollar V. Švorčík |
| author_facet | P. Slepička N. Slepičková Kasálková J. Musílková L. Bačáková B. Frýdlová P. Sajdl Z. Kolská E. Rebollar V. Švorčík |
| author_sort | P. Slepička |
| collection | DOAJ |
| description | In this study, we constructed and activated honeycomb structures on perfluorinated substrates subjected to KrF laser treatment (wavelength 248 nm). We selected the biopolymer poly L-lactic acid (PLLA) as the honeycomb material, which was dissolved in a mixture of chloroform/methanol. A micropattern of a plasma-treated perfluorethyleneperopylene (FEP) substrate was prepared by improved phase separation during dip-coating. The PLLA micropattern was subsequently treated with an excimer laser with a laser fluence of 10 mJ.cm-2 and with a different number of laser pulses. Alternatively, plasma exposure can be used as a secondary treatment. The surface morphologies of the pristine and laser-treated PLLA patterns were studied using atomic force and scanning electron microscopy. The surface chemistry was analyzed using energy-dispersive spectroscopy and X-ray photoelectron spectroscopy. In addition, the metabolic activity of adipose stem cells was evaluated using the MTS test, and cell numbers in selected samples were determined. The morphology of cells growing in the honeycomb-like pattern was studied in detail using fluorescence microscopy. In all, we used a combination of honeycomb pattern (HCP) laser treatment and plasma treatment to construct an optimal scaffold for adipose stem cell culture. |
| format | Article |
| id | doaj-art-d36e12ca44bf410faa13007c89a44cdc |
| institution | Kabale University |
| issn | 2666-5239 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Applied Surface Science Advances |
| spelling | doaj-art-d36e12ca44bf410faa13007c89a44cdc2025-01-29T05:02:03ZengElsevierApplied Surface Science Advances2666-52392025-01-0125100662PLLA honeycombs activated by plasma and high-energy excimer laser for stem cell supportP. Slepička0N. Slepičková Kasálková1J. Musílková2L. Bačáková3B. Frýdlová4P. Sajdl5Z. Kolská6E. Rebollar7V. Švorčík8Department of Solid State Engineering, University of Chemistry and Technology Prague, Technická 3, 166 28 Prague, Czech Republic; Corresponding author.Department of Solid State Engineering, University of Chemistry and Technology Prague, Technická 3, 166 28 Prague, Czech RepublicInstitute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech RepublicDepartment of Solid State Engineering, University of Chemistry and Technology Prague, Technická 3, 166 28 Prague, Czech Republic; Institute of Physiology of the Czech Academy of Sciences, Videnska 1083, 142 20 Prague 4, Czech RepublicDepartment of Solid State Engineering, University of Chemistry and Technology Prague, Technická 3, 166 28 Prague, Czech RepublicDepartment of Power Engineering, The University of Chemistry and Technology, 166 28 Prague, Czech RepublicDepartment of Solid State Engineering, University of Chemistry and Technology Prague, Technická 3, 166 28 Prague, Czech Republic; Faculty of Science, J. E. Purkyně University in Ústí nad Labem, Ústí nad Labem 40096, Czech RepublicInstituto de Química Física Blas Cabrera, CSIC, Serran 119, Madrid 28006, SpainDepartment of Solid State Engineering, University of Chemistry and Technology Prague, Technická 3, 166 28 Prague, Czech RepublicIn this study, we constructed and activated honeycomb structures on perfluorinated substrates subjected to KrF laser treatment (wavelength 248 nm). We selected the biopolymer poly L-lactic acid (PLLA) as the honeycomb material, which was dissolved in a mixture of chloroform/methanol. A micropattern of a plasma-treated perfluorethyleneperopylene (FEP) substrate was prepared by improved phase separation during dip-coating. The PLLA micropattern was subsequently treated with an excimer laser with a laser fluence of 10 mJ.cm-2 and with a different number of laser pulses. Alternatively, plasma exposure can be used as a secondary treatment. The surface morphologies of the pristine and laser-treated PLLA patterns were studied using atomic force and scanning electron microscopy. The surface chemistry was analyzed using energy-dispersive spectroscopy and X-ray photoelectron spectroscopy. In addition, the metabolic activity of adipose stem cells was evaluated using the MTS test, and cell numbers in selected samples were determined. The morphology of cells growing in the honeycomb-like pattern was studied in detail using fluorescence microscopy. In all, we used a combination of honeycomb pattern (HCP) laser treatment and plasma treatment to construct an optimal scaffold for adipose stem cell culture.http://www.sciencedirect.com/science/article/pii/S2666523924000904Honeycomb patternOrganic coatingLaser treatmentBiopolymerMorphologyCytocompatibility |
| spellingShingle | P. Slepička N. Slepičková Kasálková J. Musílková L. Bačáková B. Frýdlová P. Sajdl Z. Kolská E. Rebollar V. Švorčík PLLA honeycombs activated by plasma and high-energy excimer laser for stem cell support Applied Surface Science Advances Honeycomb pattern Organic coating Laser treatment Biopolymer Morphology Cytocompatibility |
| title | PLLA honeycombs activated by plasma and high-energy excimer laser for stem cell support |
| title_full | PLLA honeycombs activated by plasma and high-energy excimer laser for stem cell support |
| title_fullStr | PLLA honeycombs activated by plasma and high-energy excimer laser for stem cell support |
| title_full_unstemmed | PLLA honeycombs activated by plasma and high-energy excimer laser for stem cell support |
| title_short | PLLA honeycombs activated by plasma and high-energy excimer laser for stem cell support |
| title_sort | plla honeycombs activated by plasma and high energy excimer laser for stem cell support |
| topic | Honeycomb pattern Organic coating Laser treatment Biopolymer Morphology Cytocompatibility |
| url | http://www.sciencedirect.com/science/article/pii/S2666523924000904 |
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