The Effect of IFN-γ, Alum and Complete Freund Adjuvant on TNP-KLH Induced Ig.G1, IgE and IgG2a Responses in Mice
Adjuvants are considered to play an important role in directing the isotype and amount of antibodies produced upon immunization by conducting the development of either Th-1 or Th-2 cells upon T-cell stimulation. This is based on the different cytokine production patterns that were observed after in...
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| Format: | Article |
| Language: | English |
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Wiley
1994-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/S0962935194000542 |
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| author | R. van Ommen A. E. C. M. Vredendaal M. de Gooyer A. van Oudenaren H. F. J. Savelkoul |
| author_facet | R. van Ommen A. E. C. M. Vredendaal M. de Gooyer A. van Oudenaren H. F. J. Savelkoul |
| author_sort | R. van Ommen |
| collection | DOAJ |
| description | Adjuvants are considered to play an important role in directing the
isotype and amount of antibodies produced upon immunization by
conducting the development of either Th-1 or Th-2 cells upon T-cell
stimulation. This is based on the different cytokine production
patterns that were observed after in vitro resttmulation of T cells
isolated from mice immunized with antigen either adsorbed on alum or
emulsified in complete Freund adjuvant (CFA). However, other studies
suggest that primarily the type of antigen determines which isotypes
are produced and to what extent. In these studies, however, IgE was
not determined. Therefore, this study examined whether alum and CFA
influenced the amount and/or ratio of IgG1, IgE and IgG2a produced
after TNP-KLH immunization. Similar levels of IgG1, IgE and IgG2a
antibodies were found upon immunization with TNP-KLH either adsorbed
on alum or emulsified in CFA. Moreover, administration of IFN-γ in
combination with TNP-KLH adsorbed on alum did not increase the
amount of IgG2a produced. IFN-γ treatment resulted in an increased
IL-6 and decreased IFN-γ production by spleen cells upon Con A
stimulation, whereas it did not change the IL-4 production in
similar conditions. The presented results suggest that upon
immunization with TNP-KLH high IL-4 levels are produced, resulting
in an antibody response that is dominated by IgG1, independent of the
adjuvant employed. The IL-4 inducing property of TNP-KLH is
substantiated by the finding that repeated immunization of mice with
TNP-KI, without adjuvant, increases the serum total IgE level. The
presented data suggest that the carrier part of TNP-KLH
preferentially results in Th-2 cell activity after which the
adjuvant merely enhances the antibody responses generated. |
| format | Article |
| id | doaj-art-d347e1bbad50499c81dbffc2e004918e |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 1994-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-d347e1bbad50499c81dbffc2e004918e2025-02-03T01:27:46ZengWileyMediators of Inflammation0962-93511466-18611994-01-013538739210.1155/S0962935194000542The Effect of IFN-γ, Alum and Complete Freund Adjuvant on TNP-KLH Induced Ig.G1, IgE and IgG2a Responses in MiceR. van Ommen0A. E. C. M. Vredendaal1M. de Gooyer2A. van Oudenaren3H. F. J. Savelkoul4Department of Immunology, Erasmus University, PO Box 1738, Rotterdam 3000 DR, The NetherlandsDepartment of Immunology, Erasmus University, PO Box 1738, Rotterdam 3000 DR, The NetherlandsDepartment of Immunology, Erasmus University, PO Box 1738, Rotterdam 3000 DR, The NetherlandsDepartment of Immunology, Erasmus University, PO Box 1738, Rotterdam 3000 DR, The NetherlandsDepartment of Immunology, Erasmus University, PO Box 1738, Rotterdam 3000 DR, The NetherlandsAdjuvants are considered to play an important role in directing the isotype and amount of antibodies produced upon immunization by conducting the development of either Th-1 or Th-2 cells upon T-cell stimulation. This is based on the different cytokine production patterns that were observed after in vitro resttmulation of T cells isolated from mice immunized with antigen either adsorbed on alum or emulsified in complete Freund adjuvant (CFA). However, other studies suggest that primarily the type of antigen determines which isotypes are produced and to what extent. In these studies, however, IgE was not determined. Therefore, this study examined whether alum and CFA influenced the amount and/or ratio of IgG1, IgE and IgG2a produced after TNP-KLH immunization. Similar levels of IgG1, IgE and IgG2a antibodies were found upon immunization with TNP-KLH either adsorbed on alum or emulsified in CFA. Moreover, administration of IFN-γ in combination with TNP-KLH adsorbed on alum did not increase the amount of IgG2a produced. IFN-γ treatment resulted in an increased IL-6 and decreased IFN-γ production by spleen cells upon Con A stimulation, whereas it did not change the IL-4 production in similar conditions. The presented results suggest that upon immunization with TNP-KLH high IL-4 levels are produced, resulting in an antibody response that is dominated by IgG1, independent of the adjuvant employed. The IL-4 inducing property of TNP-KLH is substantiated by the finding that repeated immunization of mice with TNP-KI, without adjuvant, increases the serum total IgE level. The presented data suggest that the carrier part of TNP-KLH preferentially results in Th-2 cell activity after which the adjuvant merely enhances the antibody responses generated.http://dx.doi.org/10.1155/S0962935194000542 |
| spellingShingle | R. van Ommen A. E. C. M. Vredendaal M. de Gooyer A. van Oudenaren H. F. J. Savelkoul The Effect of IFN-γ, Alum and Complete Freund Adjuvant on TNP-KLH Induced Ig.G1, IgE and IgG2a Responses in Mice Mediators of Inflammation |
| title | The Effect of IFN-γ, Alum and Complete Freund Adjuvant on TNP-KLH Induced Ig.G1, IgE and IgG2a Responses in Mice |
| title_full | The Effect of IFN-γ, Alum and Complete Freund Adjuvant on TNP-KLH Induced Ig.G1, IgE and IgG2a Responses in Mice |
| title_fullStr | The Effect of IFN-γ, Alum and Complete Freund Adjuvant on TNP-KLH Induced Ig.G1, IgE and IgG2a Responses in Mice |
| title_full_unstemmed | The Effect of IFN-γ, Alum and Complete Freund Adjuvant on TNP-KLH Induced Ig.G1, IgE and IgG2a Responses in Mice |
| title_short | The Effect of IFN-γ, Alum and Complete Freund Adjuvant on TNP-KLH Induced Ig.G1, IgE and IgG2a Responses in Mice |
| title_sort | effect of ifn γ alum and complete freund adjuvant on tnp klh induced ig g1 ige and igg2a responses in mice |
| url | http://dx.doi.org/10.1155/S0962935194000542 |
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