The effect of <i>T. aestivum</i> chromosomes 1A and 1D on fertility of alloplasmic recombinant (<i>H. vulgare</i>)-<i>T. aestivum</i> lines depending on cytonuclear compatibility
The effect of T. aestivum L. chromosomes 1A and 1D on fertility of recombinant bread wheat allolines of the same origin carrying the cytoplasm of barley H. vulgare L. and different levels of cytonuclear compatibility was studied. Alloline L-56 included mainly fully sterile (FS) and partially sterile...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders
2024-10-01
|
| Series: | Вавиловский журнал генетики и селекции |
| Subjects: | |
| Online Access: | https://vavilov.elpub.ru/jour/article/view/4289 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832575048988229632 |
|---|---|
| author | L. A. Pershina N. V. Trubacheeva V. K. Shumny |
| author_facet | L. A. Pershina N. V. Trubacheeva V. K. Shumny |
| author_sort | L. A. Pershina |
| collection | DOAJ |
| description | The effect of T. aestivum L. chromosomes 1A and 1D on fertility of recombinant bread wheat allolines of the same origin carrying the cytoplasm of barley H. vulgare L. and different levels of cytonuclear compatibility was studied. Alloline L-56 included mainly fully sterile (FS) and partially sterile (PS) plants, alloline L-57 included partially fertile (PF) plants and line L-58 included fertile (F) ones. Analysis of morphobiological traits and pollen painting indicated complete or partial male sterility in plants of allolines L-56 and L-57. To differentiate genotypes with cytonuclear coadaptation and genotypes with cytonuclear incompatibility, PCR analysis of the 18S/5S mitochondrial (mt) repeat was performed. Heteroplasmy (simultaneous presence of barley and wheat mtDNA copies) was found in FS, PS, PF and some F plants, which was associated with a violation of cytonuclear compatibility. Wheattype homoplasmy (hm) was detected in the majority of the fertile plants, which was associated with cytonuclear coadaptation. The allolines used as maternal genotypes were crossed with wheat-rye substitution lines 1R(1A) and 1R(1D). In F1, all plants of PF×1R(1A) and PF×1R(1D) combinations were fertile, and in F2, a segregation close to 3 (fertile) : 1 (sterile) was observed. These results showed for the first time that chromosomes 1A and 1D carry one dominant Rf gene, which controls the restoration of male fertility of bread wheat carrying the cytoplasm of H. vulgare. All plants of F1 combinations FS×1R(1A), FS×1R(1D), PS×1R(1A), PS×1R(1D) were sterile, which indicates that a single dose of genes localized on wheat chromosomes 1A or 1D is not enough to restore male fertility in FS and PS plants. All plants of hybrid combinations F(hm)×1R(1A) and F(hm)×1R(1D) in both F1 and F2 were fertile, that is, fertility of allolines with cytonuclear coadaptation does not depend on wheat chromosomes 1A and 1D. |
| format | Article |
| id | doaj-art-d328283e2ab8406bb7950a6af7a0c5bd |
| institution | Kabale University |
| issn | 2500-3259 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | Siberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and Breeders |
| record_format | Article |
| series | Вавиловский журнал генетики и селекции |
| spelling | doaj-art-d328283e2ab8406bb7950a6af7a0c5bd2025-02-01T09:58:13ZengSiberian Branch of the Russian Academy of Sciences, Federal Research Center Institute of Cytology and Genetics, The Vavilov Society of Geneticists and BreedersВавиловский журнал генетики и селекции2500-32592024-10-0128661061810.18699/vjgb-24-681496The effect of <i>T. aestivum</i> chromosomes 1A and 1D on fertility of alloplasmic recombinant (<i>H. vulgare</i>)-<i>T. aestivum</i> lines depending on cytonuclear compatibilityL. A. Pershina0N. V. Trubacheeva1V. K. Shumny2Institute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Kurchatov Genomic Center of ICG SB RASInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of Sciences; Kurchatov Genomic Center of ICG SB RASInstitute of Cytology and Genetics of the Siberian Branch of the Russian Academy of SciencesThe effect of T. aestivum L. chromosomes 1A and 1D on fertility of recombinant bread wheat allolines of the same origin carrying the cytoplasm of barley H. vulgare L. and different levels of cytonuclear compatibility was studied. Alloline L-56 included mainly fully sterile (FS) and partially sterile (PS) plants, alloline L-57 included partially fertile (PF) plants and line L-58 included fertile (F) ones. Analysis of morphobiological traits and pollen painting indicated complete or partial male sterility in plants of allolines L-56 and L-57. To differentiate genotypes with cytonuclear coadaptation and genotypes with cytonuclear incompatibility, PCR analysis of the 18S/5S mitochondrial (mt) repeat was performed. Heteroplasmy (simultaneous presence of barley and wheat mtDNA copies) was found in FS, PS, PF and some F plants, which was associated with a violation of cytonuclear compatibility. Wheattype homoplasmy (hm) was detected in the majority of the fertile plants, which was associated with cytonuclear coadaptation. The allolines used as maternal genotypes were crossed with wheat-rye substitution lines 1R(1A) and 1R(1D). In F1, all plants of PF×1R(1A) and PF×1R(1D) combinations were fertile, and in F2, a segregation close to 3 (fertile) : 1 (sterile) was observed. These results showed for the first time that chromosomes 1A and 1D carry one dominant Rf gene, which controls the restoration of male fertility of bread wheat carrying the cytoplasm of H. vulgare. All plants of F1 combinations FS×1R(1A), FS×1R(1D), PS×1R(1A), PS×1R(1D) were sterile, which indicates that a single dose of genes localized on wheat chromosomes 1A or 1D is not enough to restore male fertility in FS and PS plants. All plants of hybrid combinations F(hm)×1R(1A) and F(hm)×1R(1D) in both F1 and F2 were fertile, that is, fertility of allolines with cytonuclear coadaptation does not depend on wheat chromosomes 1A and 1D.https://vavilov.elpub.ru/jour/article/view/4289allolines (h. vulgare)-t. aestivumchromosomes 1a and 1dmtdnaviolation of cytonuclear compatibilitycytonuclear coadaptationrf genes |
| spellingShingle | L. A. Pershina N. V. Trubacheeva V. K. Shumny The effect of <i>T. aestivum</i> chromosomes 1A and 1D on fertility of alloplasmic recombinant (<i>H. vulgare</i>)-<i>T. aestivum</i> lines depending on cytonuclear compatibility Вавиловский журнал генетики и селекции allolines (h. vulgare)-t. aestivum chromosomes 1a and 1d mtdna violation of cytonuclear compatibility cytonuclear coadaptation rf genes |
| title | The effect of <i>T. aestivum</i> chromosomes 1A and 1D on fertility of alloplasmic recombinant (<i>H. vulgare</i>)-<i>T. aestivum</i> lines depending on cytonuclear compatibility |
| title_full | The effect of <i>T. aestivum</i> chromosomes 1A and 1D on fertility of alloplasmic recombinant (<i>H. vulgare</i>)-<i>T. aestivum</i> lines depending on cytonuclear compatibility |
| title_fullStr | The effect of <i>T. aestivum</i> chromosomes 1A and 1D on fertility of alloplasmic recombinant (<i>H. vulgare</i>)-<i>T. aestivum</i> lines depending on cytonuclear compatibility |
| title_full_unstemmed | The effect of <i>T. aestivum</i> chromosomes 1A and 1D on fertility of alloplasmic recombinant (<i>H. vulgare</i>)-<i>T. aestivum</i> lines depending on cytonuclear compatibility |
| title_short | The effect of <i>T. aestivum</i> chromosomes 1A and 1D on fertility of alloplasmic recombinant (<i>H. vulgare</i>)-<i>T. aestivum</i> lines depending on cytonuclear compatibility |
| title_sort | effect of i t aestivum i chromosomes 1a and 1d on fertility of alloplasmic recombinant i h vulgare i i t aestivum i lines depending on cytonuclear compatibility |
| topic | allolines (h. vulgare)-t. aestivum chromosomes 1a and 1d mtdna violation of cytonuclear compatibility cytonuclear coadaptation rf genes |
| url | https://vavilov.elpub.ru/jour/article/view/4289 |
| work_keys_str_mv | AT lapershina theeffectofitaestivumichromosomes1aand1donfertilityofalloplasmicrecombinantihvulgareiitaestivumilinesdependingoncytonuclearcompatibility AT nvtrubacheeva theeffectofitaestivumichromosomes1aand1donfertilityofalloplasmicrecombinantihvulgareiitaestivumilinesdependingoncytonuclearcompatibility AT vkshumny theeffectofitaestivumichromosomes1aand1donfertilityofalloplasmicrecombinantihvulgareiitaestivumilinesdependingoncytonuclearcompatibility AT lapershina effectofitaestivumichromosomes1aand1donfertilityofalloplasmicrecombinantihvulgareiitaestivumilinesdependingoncytonuclearcompatibility AT nvtrubacheeva effectofitaestivumichromosomes1aand1donfertilityofalloplasmicrecombinantihvulgareiitaestivumilinesdependingoncytonuclearcompatibility AT vkshumny effectofitaestivumichromosomes1aand1donfertilityofalloplasmicrecombinantihvulgareiitaestivumilinesdependingoncytonuclearcompatibility |