Antioxidant and antihyperglycemic activities of Scorzonera cinerea radical leaves in streptozocin-induced diabetic rats
Scorzonera species are used for treating various diseases. They are consumed raw, especially in the spring, and have nutritious and dietetic values. This study evaluated the antidiabetic and antioxidant effects of ethanolic extracts of Scorzonera cinerea (Sc) radical leaves in diabetes mellitus. Fiv...
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2021-12-01
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| Online Access: | https://doi.org/10.2478/acph-2021-0045 |
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| author | Temiz Mehmet Ali |
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| description | Scorzonera species are used for treating various diseases. They are consumed raw, especially in the spring, and have nutritious and dietetic values. This study evaluated the antidiabetic and antioxidant effects of ethanolic extracts of Scorzonera cinerea (Sc) radical leaves in diabetes mellitus. Five random groups of Wistar rats (n = 8) were created – control, diabetic, acarbose, Sc-Dried, and Sc-Frozen. Phenolic profiles of extracts were determined by HPLC. Free radical scavenging capacity was measured using DPPH and ABTS tests. The inhibitory effects of Sc extracts on α-glucosidase and α-amylase activities were also evaluated. Moreover, superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activities, glutathione (GSH) concentration, malondialdehyde (MDA), total antioxidant status (TAS) and total oxidant status (TOS) were analyzed in the liver tissues. While dried Scorzonera extract was more effective in α-amylase inhibitory activity, frozen Scorzonera extract was more effective in α-glucosidase inhibitory activity. Sc-Dried and Sc-Frozen extracts lowered blood glucose and HbA1c levels, they also increased insulin. Although liver MDA and TOS were significantly increased in the diabetic group, their values were significantly lower in the Sc-Dried- and Sc-Frozen-treated groups. GSH, TAS, and anti-oxidant enzyme activities decreased in the diabetic group, but Sc-Dried and Sc-Frozen supplements significantly enhanced liver antioxidant values. In conclusion, S. cinerea treatment exerts potential hypoglycemic and antioxidant effects in diabetes. Thus, it can be considered as a candidate dietary supplement for health benefits in diabetes. |
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| institution | Kabale University |
| issn | 1846-9558 |
| language | English |
| publishDate | 2021-12-01 |
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| spelling | doaj-art-d31ede34005f45fba01dec614b941e392025-02-02T16:56:11ZengSciendoActa Pharmaceutica1846-95582021-12-0171460361710.2478/acph-2021-0045Antioxidant and antihyperglycemic activities of Scorzonera cinerea radical leaves in streptozocin-induced diabetic ratsTemiz Mehmet Ali0Programme of Medicinal and Aromatic Plants, Vocational School of Technical Sciences, Karamanoğlu Mehmetbey University, Karaman, TurkeyScorzonera species are used for treating various diseases. They are consumed raw, especially in the spring, and have nutritious and dietetic values. This study evaluated the antidiabetic and antioxidant effects of ethanolic extracts of Scorzonera cinerea (Sc) radical leaves in diabetes mellitus. Five random groups of Wistar rats (n = 8) were created – control, diabetic, acarbose, Sc-Dried, and Sc-Frozen. Phenolic profiles of extracts were determined by HPLC. Free radical scavenging capacity was measured using DPPH and ABTS tests. The inhibitory effects of Sc extracts on α-glucosidase and α-amylase activities were also evaluated. Moreover, superoxide dismutase (SOD), glutathione peroxidase (GPx), and catalase (CAT) activities, glutathione (GSH) concentration, malondialdehyde (MDA), total antioxidant status (TAS) and total oxidant status (TOS) were analyzed in the liver tissues. While dried Scorzonera extract was more effective in α-amylase inhibitory activity, frozen Scorzonera extract was more effective in α-glucosidase inhibitory activity. Sc-Dried and Sc-Frozen extracts lowered blood glucose and HbA1c levels, they also increased insulin. Although liver MDA and TOS were significantly increased in the diabetic group, their values were significantly lower in the Sc-Dried- and Sc-Frozen-treated groups. GSH, TAS, and anti-oxidant enzyme activities decreased in the diabetic group, but Sc-Dried and Sc-Frozen supplements significantly enhanced liver antioxidant values. In conclusion, S. cinerea treatment exerts potential hypoglycemic and antioxidant effects in diabetes. Thus, it can be considered as a candidate dietary supplement for health benefits in diabetes.https://doi.org/10.2478/acph-2021-0045scorzonera cinerearadical leavesethanolic extractantidiabeticantioxidantα-glucosidase |
| spellingShingle | Temiz Mehmet Ali Antioxidant and antihyperglycemic activities of Scorzonera cinerea radical leaves in streptozocin-induced diabetic rats Acta Pharmaceutica scorzonera cinerea radical leaves ethanolic extract antidiabetic antioxidant α-glucosidase |
| title | Antioxidant and antihyperglycemic activities of Scorzonera cinerea radical leaves in streptozocin-induced diabetic rats |
| title_full | Antioxidant and antihyperglycemic activities of Scorzonera cinerea radical leaves in streptozocin-induced diabetic rats |
| title_fullStr | Antioxidant and antihyperglycemic activities of Scorzonera cinerea radical leaves in streptozocin-induced diabetic rats |
| title_full_unstemmed | Antioxidant and antihyperglycemic activities of Scorzonera cinerea radical leaves in streptozocin-induced diabetic rats |
| title_short | Antioxidant and antihyperglycemic activities of Scorzonera cinerea radical leaves in streptozocin-induced diabetic rats |
| title_sort | antioxidant and antihyperglycemic activities of scorzonera cinerea radical leaves in streptozocin induced diabetic rats |
| topic | scorzonera cinerea radical leaves ethanolic extract antidiabetic antioxidant α-glucosidase |
| url | https://doi.org/10.2478/acph-2021-0045 |
| work_keys_str_mv | AT temizmehmetali antioxidantandantihyperglycemicactivitiesofscorzoneracinerearadicalleavesinstreptozocininduceddiabeticrats |