2,4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical Mastitis
Mastitis is a major disease in dairy cows resulting in significant economic losses. In vitro works suggest that ruminants peroxisome proliferator-activated receptor gamma (PPARγ) can aid in improving the response to mastitis and can control milk fat synthesis. The objectives of the present experimen...
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| Format: | Article |
| Language: | English |
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Wiley
2017-01-01
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| Series: | PPAR Research |
| Online Access: | http://dx.doi.org/10.1155/2017/7097450 |
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| author | Fernanda Rosa Johan S. Osorio Erminio Trevisi Francisco Yanqui-Rivera Charles T. Estill Massimo Bionaz |
| author_facet | Fernanda Rosa Johan S. Osorio Erminio Trevisi Francisco Yanqui-Rivera Charles T. Estill Massimo Bionaz |
| author_sort | Fernanda Rosa |
| collection | DOAJ |
| description | Mastitis is a major disease in dairy cows resulting in significant economic losses. In vitro works suggest that ruminants peroxisome proliferator-activated receptor gamma (PPARγ) can aid in improving the response to mastitis and can control milk fat synthesis. The objectives of the present experiment were to test if treatment with the putative PPARγ agonist 2,4-thiazolidinedione (TZD) improves (1) the response to subclinical mastitis and (2) milk fat production. Lactating goats received daily injections of 8 mg/kg BW of TZD or saline for 3 weeks. After one week of TZD injection, half of the goats in each group received intramammary infusion of Strep. uberis or saline in both halves for a total of 4 groups (n=6/group). TZD treatment did not affect milk fat but had positive effect on milk somatic cells count, blood nonesterified fatty acids, inflammatory markers, and liver function. TZD significantly increased myeloperoxidase but did not affect leukocytes phagocytosis or insulin. TZD increased adipocytes size and had minor effect on expression of PPARγ target genes in mammary epithelial cells but not in adipose tissue. Overall, TZD ameliorated the response to intramammary infection but the effect on milk fat synthesis and expression of related transcripts was less than expected. |
| format | Article |
| id | doaj-art-d31510ff04ef4770b5e15b23ffb205a9 |
| institution | Kabale University |
| issn | 1687-4757 1687-4765 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | PPAR Research |
| spelling | doaj-art-d31510ff04ef4770b5e15b23ffb205a92025-02-03T01:24:15ZengWileyPPAR Research1687-47571687-47652017-01-01201710.1155/2017/709745070974502,4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical MastitisFernanda Rosa0Johan S. Osorio1Erminio Trevisi2Francisco Yanqui-Rivera3Charles T. Estill4Massimo Bionaz5Department of Animal and Rangeland Sciences, Oregon State University, Corvallis, OR 97331, USADepartment of Animal and Rangeland Sciences, Oregon State University, Corvallis, OR 97331, USAIstituto di Zootecnica, Facoltà di Scienze Agrarie, Alimentari e Ambientali, Università Cattolica del Sacro Cuore, 29122 Piacenza, ItalyDepartment of Animal and Rangeland Sciences, Oregon State University, Corvallis, OR 97331, USADepartment of Animal and Rangeland Sciences, Oregon State University, Corvallis, OR 97331, USADepartment of Animal and Rangeland Sciences, Oregon State University, Corvallis, OR 97331, USAMastitis is a major disease in dairy cows resulting in significant economic losses. In vitro works suggest that ruminants peroxisome proliferator-activated receptor gamma (PPARγ) can aid in improving the response to mastitis and can control milk fat synthesis. The objectives of the present experiment were to test if treatment with the putative PPARγ agonist 2,4-thiazolidinedione (TZD) improves (1) the response to subclinical mastitis and (2) milk fat production. Lactating goats received daily injections of 8 mg/kg BW of TZD or saline for 3 weeks. After one week of TZD injection, half of the goats in each group received intramammary infusion of Strep. uberis or saline in both halves for a total of 4 groups (n=6/group). TZD treatment did not affect milk fat but had positive effect on milk somatic cells count, blood nonesterified fatty acids, inflammatory markers, and liver function. TZD significantly increased myeloperoxidase but did not affect leukocytes phagocytosis or insulin. TZD increased adipocytes size and had minor effect on expression of PPARγ target genes in mammary epithelial cells but not in adipose tissue. Overall, TZD ameliorated the response to intramammary infection but the effect on milk fat synthesis and expression of related transcripts was less than expected.http://dx.doi.org/10.1155/2017/7097450 |
| spellingShingle | Fernanda Rosa Johan S. Osorio Erminio Trevisi Francisco Yanqui-Rivera Charles T. Estill Massimo Bionaz 2,4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical Mastitis PPAR Research |
| title | 2,4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical Mastitis |
| title_full | 2,4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical Mastitis |
| title_fullStr | 2,4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical Mastitis |
| title_full_unstemmed | 2,4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical Mastitis |
| title_short | 2,4-Thiazolidinedione Treatment Improves the Innate Immune Response in Dairy Goats with Induced Subclinical Mastitis |
| title_sort | 2 4 thiazolidinedione treatment improves the innate immune response in dairy goats with induced subclinical mastitis |
| url | http://dx.doi.org/10.1155/2017/7097450 |
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