Effects of bFGF on the Modulation of Apoptosis in Gingival Fibroblasts with Different Host Ages
The purpose of this study was to investigate the effects of basic fibroblast growth factor (bFGF) treatment on the proliferation and apoptosis of cultured gingival fibroblasts (GFs). Human GFs were isolated from the palatal gingival tissues of 16 healthy volunteers ranging in the age from 9 to 35 ye...
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| Main Authors: | , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | International Journal of Dentistry |
| Online Access: | http://dx.doi.org/10.1155/2013/619580 |
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| author | Kotaro Tanimoto Satoru Ohkuma Yuki Tanne Ryo Kunimatsu Naoto Hirose Tomomi Mitsuyoshi Yuki Yoshimi Shaoching Su Kazuo Tanne |
| author_facet | Kotaro Tanimoto Satoru Ohkuma Yuki Tanne Ryo Kunimatsu Naoto Hirose Tomomi Mitsuyoshi Yuki Yoshimi Shaoching Su Kazuo Tanne |
| author_sort | Kotaro Tanimoto |
| collection | DOAJ |
| description | The purpose of this study was to investigate the effects of basic fibroblast growth factor (bFGF) treatment on the proliferation and apoptosis of cultured gingival fibroblasts (GFs). Human GFs were isolated from the palatal gingival tissues of 16 healthy volunteers ranging in the age from 9 to 35 years old. Cultured GFs were subjected to the analyses for cell proliferation by ELISA assay, gene expression by RT-PCR analysis, and apoptosis potency by caspase-3 assay. The cell proliferation activity and gene expression of type-I collagen and caspase-3 activity were enhanced significantly by the treatment with bFGF in cultured GFs. Furthermore, the activity of caspase-3 in cultured GFs from young subjects was significantly higher than that in GFs from adults. It is shown that bFGF significantly enhances the gene expression of type-I collagen in cultured fibroblasts from human gingival tissues. It also demonstrated that bFGF modulates the apoptosis of periodontal fibroblasts, and the effect is higher in young subjects, indicating a significant role of bFGF in the prevention of scar formation during wound healing. |
| format | Article |
| id | doaj-art-d2fd334fbb1f436aaa24f661e5210cb0 |
| institution | Kabale University |
| issn | 1687-8728 1687-8736 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Dentistry |
| spelling | doaj-art-d2fd334fbb1f436aaa24f661e5210cb02025-02-03T05:57:12ZengWileyInternational Journal of Dentistry1687-87281687-87362013-01-01201310.1155/2013/619580619580Effects of bFGF on the Modulation of Apoptosis in Gingival Fibroblasts with Different Host AgesKotaro Tanimoto0Satoru Ohkuma1Yuki Tanne2Ryo Kunimatsu3Naoto Hirose4Tomomi Mitsuyoshi5Yuki Yoshimi6Shaoching Su7Kazuo Tanne8Department of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, JapanDepartment of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, JapanDepartment of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, JapanDepartment of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, JapanDepartment of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, JapanDepartment of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, JapanDepartment of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, JapanDepartment of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, JapanDepartment of Orthodontics, Applied Life Sciences, Hiroshima University Institute of Biomedical & Health Sciences, 1-2-3 Kasumi, Minami-ku, Hiroshima 734-8553, JapanThe purpose of this study was to investigate the effects of basic fibroblast growth factor (bFGF) treatment on the proliferation and apoptosis of cultured gingival fibroblasts (GFs). Human GFs were isolated from the palatal gingival tissues of 16 healthy volunteers ranging in the age from 9 to 35 years old. Cultured GFs were subjected to the analyses for cell proliferation by ELISA assay, gene expression by RT-PCR analysis, and apoptosis potency by caspase-3 assay. The cell proliferation activity and gene expression of type-I collagen and caspase-3 activity were enhanced significantly by the treatment with bFGF in cultured GFs. Furthermore, the activity of caspase-3 in cultured GFs from young subjects was significantly higher than that in GFs from adults. It is shown that bFGF significantly enhances the gene expression of type-I collagen in cultured fibroblasts from human gingival tissues. It also demonstrated that bFGF modulates the apoptosis of periodontal fibroblasts, and the effect is higher in young subjects, indicating a significant role of bFGF in the prevention of scar formation during wound healing.http://dx.doi.org/10.1155/2013/619580 |
| spellingShingle | Kotaro Tanimoto Satoru Ohkuma Yuki Tanne Ryo Kunimatsu Naoto Hirose Tomomi Mitsuyoshi Yuki Yoshimi Shaoching Su Kazuo Tanne Effects of bFGF on the Modulation of Apoptosis in Gingival Fibroblasts with Different Host Ages International Journal of Dentistry |
| title | Effects of bFGF on the Modulation of Apoptosis in Gingival Fibroblasts with Different Host Ages |
| title_full | Effects of bFGF on the Modulation of Apoptosis in Gingival Fibroblasts with Different Host Ages |
| title_fullStr | Effects of bFGF on the Modulation of Apoptosis in Gingival Fibroblasts with Different Host Ages |
| title_full_unstemmed | Effects of bFGF on the Modulation of Apoptosis in Gingival Fibroblasts with Different Host Ages |
| title_short | Effects of bFGF on the Modulation of Apoptosis in Gingival Fibroblasts with Different Host Ages |
| title_sort | effects of bfgf on the modulation of apoptosis in gingival fibroblasts with different host ages |
| url | http://dx.doi.org/10.1155/2013/619580 |
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