Pentosan polysulfate ameliorates proliferation by suppressing the activation of P38MAPK in high glucose-treated HK-2 cells
Objective To investigate the effect of pentosan polysulfate(PPS) on diabetic nephropathy renal tubular epithelial cells injury through regulating p38MAPK signaling pathway.Methods Human proximal tubular epithelial cell line(HK-2) cells were grown in media with high glucose concentrationGO,20,30,40 a...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | zho |
| Published: |
Editorial Department of Journal of Clinical Nephrology
2016-01-01
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| Series: | Linchuang shenzangbing zazhi |
| Subjects: | |
| Online Access: | http://www.lcszb.com/thesisDetails?columnId=57918218&Fpath=home&index=0 |
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| Summary: | Objective To investigate the effect of pentosan polysulfate(PPS) on diabetic nephropathy renal tubular epithelial cells injury through regulating p38MAPK signaling pathway.Methods Human proximal tubular epithelial cell line(HK-2) cells were grown in media with high glucose concentrationGO,20,30,40 and 50 mmol/L).The rate of cell proliferation was examined by the CCK-8 method.The HK-2 cells were grown in media with high glucose(30 mmol/L) for 30 min,1 h,2 h,4 h,6 h,12 h,24 h and 48 h,and the expression of p38MAPK proteins in the HK-2 cells was analyzed by Western blotting.The HK-2 cells were divided into:controKCON) group,high glucose(30 mmol/L,HG) group,PPS(200 fig/ml) group,HG plus PPS group,p38MAPK inhibitor SB202190(20 μmol/L,SB) group,SB plus HG(SB + HG group),and SB plus PPS group.After pretreatment for 48 h,the rate of cell proliferation was examined.After pretreatment for 6 h,the expression of p38MAPK proteins in the HK-2 cells was analyzed.Results As compared with the CON group,the rate of cell proliferation in the HG group(30 mmol/L) was increased significantly.Simultaneous incubation with PPS inhibited HK-2 cell proliferation(P<0.01 for all).As compared with the CON group,the high glucose increased the phosphorylation of p38MAPK in HK-2 cells for 6 h(P<0.01 for all).The expression of phosphorylated p38(p-p38MAPK) protein in the HG+ PPS group was significantly decreased as compared with that in the HG group(P<0.01 for all).The HK-2 cells were treated with p38MAPK inhibitor SB202190 at the dose of 20 μmol/L for 6 h.As compared with the HG group,the p38MAPK phosphorylation levels in the HG+ SB group was decreased(P<0.05).The expression of p-p38MAPK protein in the PPS + SB group was significantly decreased as compared with that in the PPS group(P<0.05 for all).Conclusions This data demonstrate that blocking p38MAPK signal transduction pathway specifically could inhibit the proliferation of HK-2cells and exert a protective effect on diabetic nephropathy. |
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| ISSN: | 1671-2390 |