Characterization and PCR Application of Family B DNA Polymerases from <i>Thermococcus stetteri</i>

Characterization and PCR Application of Family B DNA Polymerases from <i>Thermococcus stetteri</i>

DNA polymerases from the hyperthermophilic Archaea have attracted considerable attention as PCR enzymes due to their high thermal stability and proofreading 3′ → 5′ exonuclease activity. This study is the first to report data concerning the purification and biochemical characteristics of the Tst DNA...

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Bibliographic Details
Main Authors: Aleksandra A. Kuznetsova, Marina A. Soloveva, Elena S. Mikushina, Anastasia A. Gavrilova, Artemiy S. Bakman, Nikita A. Kuznetsov
Format: Article
Language:English
Published: MDPI AG 2024-11-01
Series:Life
Subjects:
PCR
<i>Thermococcus stetteri</i>
fusion DNA polymerase
mutagenesis
enzyme activity
family B
Online Access:https://www.mdpi.com/2075-1729/14/12/1544
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