RETRACTED: In Vitro Neuroprotective Effect Evaluation of Donepezil‐Loaded PLGA Nanoparticles‐Embedded PVA/PEG Nanofibers on SH‐SY5Y Cells and AP‐APP Plasmid Related Alzheimer Cell Line Model
Abstract Recently developed nanoparticles and nanofibers present new brain‐specific treatment strategies, especially for Alzheimer's disease treatment. In this study, donepezil (DO)‐loaded PLGA nanoparticles (DNP) are embedded in PVA/PEG nanofibers (DNPF) produced by pressurized gyration for su...
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| Format: | Article |
| Language: | English |
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Wiley-VCH
2025-03-01
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| Series: | Macromolecular Materials and Engineering |
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| Online Access: | https://doi.org/10.1002/mame.202400160 |
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| author | Ece Guler Humeyra Betul Yekeler Burcu Uner Murat Dogan Asima Asghar Fakhera Ikram Yusufhan Yazir Oguzhan Gunduz Deepak M Kalaskar Muhammet Emin Cam |
| author_facet | Ece Guler Humeyra Betul Yekeler Burcu Uner Murat Dogan Asima Asghar Fakhera Ikram Yusufhan Yazir Oguzhan Gunduz Deepak M Kalaskar Muhammet Emin Cam |
| author_sort | Ece Guler |
| collection | DOAJ |
| description | Abstract Recently developed nanoparticles and nanofibers present new brain‐specific treatment strategies, especially for Alzheimer's disease treatment. In this study, donepezil (DO)‐loaded PLGA nanoparticles (DNP) are embedded in PVA/PEG nanofibers (DNPF) produced by pressurized gyration for sublingual administration. SEM images showed produced drug‐loaded and pure nanofibers, which have sizes between 978 and 1123 nm, demonstrated beadless morphology and homogeneous distribution. FT‐IR, XRD, and DSC results proved the produced nanoparticles and fibers to consist of the DO and other polymers. The in vitro drug release test presented that the release profile of DO is completed at the end of the 18th day. It is released by the first order kinetic model. DNPF has an ultra‐fast release profile via its disintegration within 2 sec, which proved itself to be suitable for the administration sublingually. All samples presented above ≈90% cell viability via their non‐toxic natures on SH‐SY5Y human neuroblastoma cells by using Alamar blue assay. The anti‐Alzheimer effects of DO, DNP, and DNPF are evaluated on the Aβ1−42‐induced SH‐SY5Y cells at 1, 5, and 10 µM as treatment groups. The 1 µM dosage exhibited the most significant neuroprotective effects, which showed enhanced cellular uptake and superior modulation of Alzheimer's‐related proteins, including tau and Aβ. |
| format | Article |
| id | doaj-art-d2e0d1ac8eaa4d8aad41ebc66ef00a8d |
| institution | Kabale University |
| issn | 1438-7492 1439-2054 |
| language | English |
| publishDate | 2025-03-01 |
| publisher | Wiley-VCH |
| record_format | Article |
| series | Macromolecular Materials and Engineering |
| spelling | doaj-art-d2e0d1ac8eaa4d8aad41ebc66ef00a8d2025-08-20T03:27:13ZengWiley-VCHMacromolecular Materials and Engineering1438-74921439-20542025-03-013103n/an/a10.1002/mame.202400160RETRACTED: In Vitro Neuroprotective Effect Evaluation of Donepezil‐Loaded PLGA Nanoparticles‐Embedded PVA/PEG Nanofibers on SH‐SY5Y Cells and AP‐APP Plasmid Related Alzheimer Cell Line ModelEce Guler0Humeyra Betul Yekeler1Burcu Uner2Murat Dogan3Asima Asghar4Fakhera Ikram5Yusufhan Yazir6Oguzhan Gunduz7Deepak M Kalaskar8Muhammet Emin Cam9Department of Pharmacology, School of Pharmacy Istanbul Kent University Istanbul 34406 Kagithane TürkiyeCenter for Nanotechnology and Biomaterials Application and Research Marmara University Istanbul 34722 TürkiyeMecNano Technologies Cube Incubation Istanbul 34906 Teknopark İstanbul TürkiyeDepartment of Pharmaceutical Biotechnology Faculty of Pharmacy Sivas Cumhuriyet University Sivas 58140 TürkiyeInterdisciplinary Research Centre in Biomedical Materials COMSATS University Islamabad Lahore Campus PakistanInterdisciplinary Research Centre in Biomedical Materials COMSATS University Islamabad Lahore Campus PakistanStem Cell and Gene Therapies Research and Applied Center, Medical Faculty Kocaeli University Kocaeli 41380 TurkiyeCenter for Nanotechnology and Biomaterials Application and Research Marmara University Istanbul 34722 TürkiyeUCL Division of Surgery and Interventional Sciences Rowland Hill Street London NW3 2PF UKDepartment of Pharmacology, School of Pharmacy Istanbul Kent University Istanbul 34406 Kagithane TürkiyeAbstract Recently developed nanoparticles and nanofibers present new brain‐specific treatment strategies, especially for Alzheimer's disease treatment. In this study, donepezil (DO)‐loaded PLGA nanoparticles (DNP) are embedded in PVA/PEG nanofibers (DNPF) produced by pressurized gyration for sublingual administration. SEM images showed produced drug‐loaded and pure nanofibers, which have sizes between 978 and 1123 nm, demonstrated beadless morphology and homogeneous distribution. FT‐IR, XRD, and DSC results proved the produced nanoparticles and fibers to consist of the DO and other polymers. The in vitro drug release test presented that the release profile of DO is completed at the end of the 18th day. It is released by the first order kinetic model. DNPF has an ultra‐fast release profile via its disintegration within 2 sec, which proved itself to be suitable for the administration sublingually. All samples presented above ≈90% cell viability via their non‐toxic natures on SH‐SY5Y human neuroblastoma cells by using Alamar blue assay. The anti‐Alzheimer effects of DO, DNP, and DNPF are evaluated on the Aβ1−42‐induced SH‐SY5Y cells at 1, 5, and 10 µM as treatment groups. The 1 µM dosage exhibited the most significant neuroprotective effects, which showed enhanced cellular uptake and superior modulation of Alzheimer's‐related proteins, including tau and Aβ.https://doi.org/10.1002/mame.202400160Alzheimer's diseaseAlzheimer's disease cell modelAP‐APP plasmid transfectiondonepezildrug deliverynanofiber |
| spellingShingle | Ece Guler Humeyra Betul Yekeler Burcu Uner Murat Dogan Asima Asghar Fakhera Ikram Yusufhan Yazir Oguzhan Gunduz Deepak M Kalaskar Muhammet Emin Cam RETRACTED: In Vitro Neuroprotective Effect Evaluation of Donepezil‐Loaded PLGA Nanoparticles‐Embedded PVA/PEG Nanofibers on SH‐SY5Y Cells and AP‐APP Plasmid Related Alzheimer Cell Line Model Macromolecular Materials and Engineering Alzheimer's disease Alzheimer's disease cell model AP‐APP plasmid transfection donepezil drug delivery nanofiber |
| title | RETRACTED: In Vitro Neuroprotective Effect Evaluation of Donepezil‐Loaded PLGA Nanoparticles‐Embedded PVA/PEG Nanofibers on SH‐SY5Y Cells and AP‐APP Plasmid Related Alzheimer Cell Line Model |
| title_full | RETRACTED: In Vitro Neuroprotective Effect Evaluation of Donepezil‐Loaded PLGA Nanoparticles‐Embedded PVA/PEG Nanofibers on SH‐SY5Y Cells and AP‐APP Plasmid Related Alzheimer Cell Line Model |
| title_fullStr | RETRACTED: In Vitro Neuroprotective Effect Evaluation of Donepezil‐Loaded PLGA Nanoparticles‐Embedded PVA/PEG Nanofibers on SH‐SY5Y Cells and AP‐APP Plasmid Related Alzheimer Cell Line Model |
| title_full_unstemmed | RETRACTED: In Vitro Neuroprotective Effect Evaluation of Donepezil‐Loaded PLGA Nanoparticles‐Embedded PVA/PEG Nanofibers on SH‐SY5Y Cells and AP‐APP Plasmid Related Alzheimer Cell Line Model |
| title_short | RETRACTED: In Vitro Neuroprotective Effect Evaluation of Donepezil‐Loaded PLGA Nanoparticles‐Embedded PVA/PEG Nanofibers on SH‐SY5Y Cells and AP‐APP Plasmid Related Alzheimer Cell Line Model |
| title_sort | retracted in vitro neuroprotective effect evaluation of donepezil loaded plga nanoparticles embedded pva peg nanofibers on sh sy5y cells and ap app plasmid related alzheimer cell line model |
| topic | Alzheimer's disease Alzheimer's disease cell model AP‐APP plasmid transfection donepezil drug delivery nanofiber |
| url | https://doi.org/10.1002/mame.202400160 |
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