Comparison of Modified Chandler, Roller Pump, and Ball Valve Circulation Models for In Vitro Testing in High Blood Flow Conditions: Application in Thrombogenicity Testing of Different Materials for Vascular Applications
Three different models, a modified Chandler loop, roller pump, and a new ball valve model (Hemobile), were compared with regard to intrinsic damage of blood components and activation of platelets. The Hemobile was used for testing of polymer tubes. High flow was not possible with the Chandler loop....
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | International Journal of Biomaterials |
| Online Access: | http://dx.doi.org/10.1155/2012/673163 |
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| author | Wim van Oeveren Ignace F. Tielliu Jurgen de Hart |
| author_facet | Wim van Oeveren Ignace F. Tielliu Jurgen de Hart |
| author_sort | Wim van Oeveren |
| collection | DOAJ |
| description | Three different models, a modified Chandler loop, roller pump, and a new ball valve model (Hemobile), were compared with regard to intrinsic damage of blood components and activation of platelets. The Hemobile was used for testing of polymer tubes.
High flow was not possible with the Chandler loop. The roller pump and the Hemobile could be adjusted to high flow, but he pump induced hemolysis. Platelet numbers were reduced in the roller pump and Chandler loop (P<0.05), but remained high in the Hemobile. Platelet aggregation was reduced in all models.
The Hemobile was applied for testing vascular graft materials, and allowed different circuits circulated simultaneously at 37°C. ePTFE, Dyneema Purity UHMWPE fiber and PET fiber based tubes, all showed hemolysis below 0.2% and reduced platelet count and function. Binding of fibrin and platelets was higer on PET, inflammatory markers were lowest on Dyneema Purity UHMWPE.
We concluded that the Hemobile minimally affects blood and could be adjusted to high blood flows, simulating arterial shear stress. The Hemobile was used to measure hemocompatibility of graft material and showed Dyneema Purity UHMWPE fiber in many ways more hemocompatible than ePTFE and PET. |
| format | Article |
| id | doaj-art-d2cc832b1605447da1c3b774863394c4 |
| institution | Kabale University |
| issn | 1687-8787 1687-8795 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Biomaterials |
| spelling | doaj-art-d2cc832b1605447da1c3b774863394c42025-02-03T01:23:47ZengWileyInternational Journal of Biomaterials1687-87871687-87952012-01-01201210.1155/2012/673163673163Comparison of Modified Chandler, Roller Pump, and Ball Valve Circulation Models for In Vitro Testing in High Blood Flow Conditions: Application in Thrombogenicity Testing of Different Materials for Vascular ApplicationsWim van Oeveren0Ignace F. Tielliu1Jurgen de Hart2Haemoscan and Department of Cardiothoracic Surgery, UMCG, Haemoscan, Stavangerweg 23-23, 9723 JC, Groningen, The NetherlandsUniversity Medical Center Groningen (UMCG), P.O. Box 9700 RB Groningen, The NetherlandsHemolab, Den Dolech 2, 5612 AZ Eindhoven, The NetherlandsThree different models, a modified Chandler loop, roller pump, and a new ball valve model (Hemobile), were compared with regard to intrinsic damage of blood components and activation of platelets. The Hemobile was used for testing of polymer tubes. High flow was not possible with the Chandler loop. The roller pump and the Hemobile could be adjusted to high flow, but he pump induced hemolysis. Platelet numbers were reduced in the roller pump and Chandler loop (P<0.05), but remained high in the Hemobile. Platelet aggregation was reduced in all models. The Hemobile was applied for testing vascular graft materials, and allowed different circuits circulated simultaneously at 37°C. ePTFE, Dyneema Purity UHMWPE fiber and PET fiber based tubes, all showed hemolysis below 0.2% and reduced platelet count and function. Binding of fibrin and platelets was higer on PET, inflammatory markers were lowest on Dyneema Purity UHMWPE. We concluded that the Hemobile minimally affects blood and could be adjusted to high blood flows, simulating arterial shear stress. The Hemobile was used to measure hemocompatibility of graft material and showed Dyneema Purity UHMWPE fiber in many ways more hemocompatible than ePTFE and PET.http://dx.doi.org/10.1155/2012/673163 |
| spellingShingle | Wim van Oeveren Ignace F. Tielliu Jurgen de Hart Comparison of Modified Chandler, Roller Pump, and Ball Valve Circulation Models for In Vitro Testing in High Blood Flow Conditions: Application in Thrombogenicity Testing of Different Materials for Vascular Applications International Journal of Biomaterials |
| title | Comparison of Modified Chandler, Roller Pump, and Ball Valve Circulation Models for In Vitro Testing in High Blood Flow Conditions: Application in Thrombogenicity Testing of Different Materials for Vascular Applications |
| title_full | Comparison of Modified Chandler, Roller Pump, and Ball Valve Circulation Models for In Vitro Testing in High Blood Flow Conditions: Application in Thrombogenicity Testing of Different Materials for Vascular Applications |
| title_fullStr | Comparison of Modified Chandler, Roller Pump, and Ball Valve Circulation Models for In Vitro Testing in High Blood Flow Conditions: Application in Thrombogenicity Testing of Different Materials for Vascular Applications |
| title_full_unstemmed | Comparison of Modified Chandler, Roller Pump, and Ball Valve Circulation Models for In Vitro Testing in High Blood Flow Conditions: Application in Thrombogenicity Testing of Different Materials for Vascular Applications |
| title_short | Comparison of Modified Chandler, Roller Pump, and Ball Valve Circulation Models for In Vitro Testing in High Blood Flow Conditions: Application in Thrombogenicity Testing of Different Materials for Vascular Applications |
| title_sort | comparison of modified chandler roller pump and ball valve circulation models for in vitro testing in high blood flow conditions application in thrombogenicity testing of different materials for vascular applications |
| url | http://dx.doi.org/10.1155/2012/673163 |
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