New Platform Technology for Comprehensive Serological Diagnostics of Autoimmune Diseases
Antibody assessment is an essential part in the serological diagnosis of autoimmune diseases. However, different diagnostic strategies have been proposed for the work up of sera in particular from patients with systemic autoimmune rheumatic disease (SARD). In general, screening for SARD-associated a...
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| Format: | Article |
| Language: | English |
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Wiley
2012-01-01
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| Series: | Clinical and Developmental Immunology |
| Online Access: | http://dx.doi.org/10.1155/2012/284740 |
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| author | Annika Willitzki Rico Hiemann Vanessa Peters Ulrich Sack Peter Schierack Stefan Rödiger Ursula Anderer Karsten Conrad Dimitrios P. Bogdanos Dirk Reinhold Dirk Roggenbuck |
| author_facet | Annika Willitzki Rico Hiemann Vanessa Peters Ulrich Sack Peter Schierack Stefan Rödiger Ursula Anderer Karsten Conrad Dimitrios P. Bogdanos Dirk Reinhold Dirk Roggenbuck |
| author_sort | Annika Willitzki |
| collection | DOAJ |
| description | Antibody assessment is an essential part in the serological diagnosis of autoimmune diseases. However, different diagnostic strategies have been proposed for the work up of sera in particular from patients with systemic autoimmune rheumatic disease (SARD). In general, screening for SARD-associated antibodies by indirect immunofluorescence (IIF) is followed by confirmatory testing covering different assay techniques. Due to lacking automation, standardization, modern data management, and human bias in IIF screening, this two-stage approach has recently been challenged by multiplex techniques particularly in laboratories with high workload. However, detection of antinuclear antibodies by IIF is still recommended to be the gold standard method for antibody screening in sera from patients with suspected SARD. To address the limitations of IIF and to meet the demand for cost-efficient autoantibody screening, automated IIF methods employing novel pattern recognition algorithms for image analysis have been introduced recently. In this respect, the AKLIDES technology has been the first commercially available platform for automated interpretation of cell-based IIF testing and provides multiplexing by addressable microbead immunoassays for confirmatory testing. This paper gives an overview of recently published studies demonstrating the advantages of this new technology for SARD serology. |
| format | Article |
| id | doaj-art-d2cbc4374bc54d86b2f1b21bac4bd7b6 |
| institution | Kabale University |
| issn | 1740-2522 1740-2530 |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Clinical and Developmental Immunology |
| spelling | doaj-art-d2cbc4374bc54d86b2f1b21bac4bd7b62025-02-03T01:22:54ZengWileyClinical and Developmental Immunology1740-25221740-25302012-01-01201210.1155/2012/284740284740New Platform Technology for Comprehensive Serological Diagnostics of Autoimmune DiseasesAnnika Willitzki0Rico Hiemann1Vanessa Peters2Ulrich Sack3Peter Schierack4Stefan Rödiger5Ursula Anderer6Karsten Conrad7Dimitrios P. Bogdanos8Dirk Reinhold9Dirk Roggenbuck10Institute of Molecular and Clinical Immunology, Otto-von-Guericke University, 39120 Magdeburg, GermanyFaculty of Natural Sciences, Lausitz University of Applied Sciences, 01968 Senftenberg, GermanyR/D, Medipan GmbH, 15827 Dahlewitz/Berlin, GermanyInstitute of Clinical Immunology, Medical Faculty, University of Leipzig, 04103 Leipzig, GermanyFaculty of Natural Sciences, Lausitz University of Applied Sciences, 01968 Senftenberg, GermanyFaculty of Natural Sciences, Lausitz University of Applied Sciences, 01968 Senftenberg, GermanyFaculty of Natural Sciences, Lausitz University of Applied Sciences, 01968 Senftenberg, GermanyInstitute of Immunology, Technical University, 01307 Dresden, GermanyDivision of Transplantation Immunology and Mucosal Biology, King's College London School of Medicine, King's College Hospital, Denmark Hill Campus, Bessemer Road, London SE5 9RJ, UKInstitute of Molecular and Clinical Immunology, Otto-von-Guericke University, 39120 Magdeburg, GermanyFaculty of Natural Sciences, Lausitz University of Applied Sciences, 01968 Senftenberg, GermanyAntibody assessment is an essential part in the serological diagnosis of autoimmune diseases. However, different diagnostic strategies have been proposed for the work up of sera in particular from patients with systemic autoimmune rheumatic disease (SARD). In general, screening for SARD-associated antibodies by indirect immunofluorescence (IIF) is followed by confirmatory testing covering different assay techniques. Due to lacking automation, standardization, modern data management, and human bias in IIF screening, this two-stage approach has recently been challenged by multiplex techniques particularly in laboratories with high workload. However, detection of antinuclear antibodies by IIF is still recommended to be the gold standard method for antibody screening in sera from patients with suspected SARD. To address the limitations of IIF and to meet the demand for cost-efficient autoantibody screening, automated IIF methods employing novel pattern recognition algorithms for image analysis have been introduced recently. In this respect, the AKLIDES technology has been the first commercially available platform for automated interpretation of cell-based IIF testing and provides multiplexing by addressable microbead immunoassays for confirmatory testing. This paper gives an overview of recently published studies demonstrating the advantages of this new technology for SARD serology.http://dx.doi.org/10.1155/2012/284740 |
| spellingShingle | Annika Willitzki Rico Hiemann Vanessa Peters Ulrich Sack Peter Schierack Stefan Rödiger Ursula Anderer Karsten Conrad Dimitrios P. Bogdanos Dirk Reinhold Dirk Roggenbuck New Platform Technology for Comprehensive Serological Diagnostics of Autoimmune Diseases Clinical and Developmental Immunology |
| title | New Platform Technology for Comprehensive Serological Diagnostics of Autoimmune Diseases |
| title_full | New Platform Technology for Comprehensive Serological Diagnostics of Autoimmune Diseases |
| title_fullStr | New Platform Technology for Comprehensive Serological Diagnostics of Autoimmune Diseases |
| title_full_unstemmed | New Platform Technology for Comprehensive Serological Diagnostics of Autoimmune Diseases |
| title_short | New Platform Technology for Comprehensive Serological Diagnostics of Autoimmune Diseases |
| title_sort | new platform technology for comprehensive serological diagnostics of autoimmune diseases |
| url | http://dx.doi.org/10.1155/2012/284740 |
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