Black mulberry (Morus nigra L.) prevents deleterious effects of excess glucose in obese C. elegans decreasing lipofuscin accumulation and ROS production
Black mulberries have been traditionally used as antidiabetic agents and are a source of nutrients and phenolic compounds, particularly anthocyanins. The objective of this work is to determine if Morus nigra berries could prevent metabolic and obesity-related disorders using in vitro systems and in...
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Elsevier
2025-01-01
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405844025002786 |
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| author | Sonia Núñez Adrián Millán-Laleona Javier Cano-Lou Andrea Corella Cristina Moliner Guillermo Cásedas Filippo Maggi Víctor López Carlota Gómez-Rincón |
| author_facet | Sonia Núñez Adrián Millán-Laleona Javier Cano-Lou Andrea Corella Cristina Moliner Guillermo Cásedas Filippo Maggi Víctor López Carlota Gómez-Rincón |
| author_sort | Sonia Núñez |
| collection | DOAJ |
| description | Black mulberries have been traditionally used as antidiabetic agents and are a source of nutrients and phenolic compounds, particularly anthocyanins. The objective of this work is to determine if Morus nigra berries could prevent metabolic and obesity-related disorders using in vitro systems and in vivo alternative models such as C. elegans. An aqueous solvent-free extract from Morus nigra fruits rich in phenolic compounds like chlorogenic acid, hyperoside, rutin and cyanidin 3-glucoside was evaluated in the C. elegans obese model subjected to high glucose concentrations evaluating different parameters such as lipid droplets, lipofuscin accumulation and ROS production. The capacity of the extract to inhibit advance glycation end products and free radicals as well as pancreatic lipase and α-amylase was also evaluated in vitro. The black mulberry extract showed a significant capacity to inhibit the accumulation of lipid droplets, reducing by 50.40 % the fat deposits. The extract was able to reverse the deleterious effects of excess glucose in C. elegans enhancing stress resistance, preventing the accumulation of lipofuscin, and decreasing the ROS production. The anti-glycation and antioxidant effects in vitro were higher than the reference substances aminoguanidine and quercetin respectively. Morus nigra was also able to inhibit the pancreatic enzymes α-amylase and lipase and could be considered an interesting traditional food ingredient in the prevention of certain metabolic diseases. |
| format | Article |
| id | doaj-art-d2c31fb3f5784ff9bef9357bb2b33aa8 |
| institution | Kabale University |
| issn | 2405-8440 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Heliyon |
| spelling | doaj-art-d2c31fb3f5784ff9bef9357bb2b33aa82025-02-02T05:28:30ZengElsevierHeliyon2405-84402025-01-01112e41898Black mulberry (Morus nigra L.) prevents deleterious effects of excess glucose in obese C. elegans decreasing lipofuscin accumulation and ROS productionSonia Núñez0Adrián Millán-Laleona1Javier Cano-Lou2Andrea Corella3Cristina Moliner4Guillermo Cásedas5Filippo Maggi6Víctor López7Carlota Gómez-Rincón8Department of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, Villanueva de Gállego, 50830, Zaragoza, Spain; Instituto Agroalimentario de Aragón-IA2, CITA-Universidad de Zaragoza, 50013, Zaragoza, SpainDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, Villanueva de Gállego, 50830, Zaragoza, Spain; Instituto Agroalimentario de Aragón-IA2, CITA-Universidad de Zaragoza, 50013, Zaragoza, SpainDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, Villanueva de Gállego, 50830, Zaragoza, SpainDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, Villanueva de Gállego, 50830, Zaragoza, SpainDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, Villanueva de Gállego, 50830, Zaragoza, SpainDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, Villanueva de Gállego, 50830, Zaragoza, Spain; Instituto Agroalimentario de Aragón-IA2, CITA-Universidad de Zaragoza, 50013, Zaragoza, SpainSchool of Pharmacy, Chemistry Interdisciplinary Project (ChIP), University of Camerino, Camerino, 62032, ItalyDepartment of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, Villanueva de Gállego, 50830, Zaragoza, Spain; Instituto Agroalimentario de Aragón-IA2, CITA-Universidad de Zaragoza, 50013, Zaragoza, Spain; Corresponding author. Universidad San Jorge, Villanueva de Gállego, 50830, Zaragoza, Spain.Department of Pharmacy, Faculty of Health Sciences, Universidad San Jorge, Villanueva de Gállego, 50830, Zaragoza, Spain; Instituto Agroalimentario de Aragón-IA2, CITA-Universidad de Zaragoza, 50013, Zaragoza, SpainBlack mulberries have been traditionally used as antidiabetic agents and are a source of nutrients and phenolic compounds, particularly anthocyanins. The objective of this work is to determine if Morus nigra berries could prevent metabolic and obesity-related disorders using in vitro systems and in vivo alternative models such as C. elegans. An aqueous solvent-free extract from Morus nigra fruits rich in phenolic compounds like chlorogenic acid, hyperoside, rutin and cyanidin 3-glucoside was evaluated in the C. elegans obese model subjected to high glucose concentrations evaluating different parameters such as lipid droplets, lipofuscin accumulation and ROS production. The capacity of the extract to inhibit advance glycation end products and free radicals as well as pancreatic lipase and α-amylase was also evaluated in vitro. The black mulberry extract showed a significant capacity to inhibit the accumulation of lipid droplets, reducing by 50.40 % the fat deposits. The extract was able to reverse the deleterious effects of excess glucose in C. elegans enhancing stress resistance, preventing the accumulation of lipofuscin, and decreasing the ROS production. The anti-glycation and antioxidant effects in vitro were higher than the reference substances aminoguanidine and quercetin respectively. Morus nigra was also able to inhibit the pancreatic enzymes α-amylase and lipase and could be considered an interesting traditional food ingredient in the prevention of certain metabolic diseases.http://www.sciencedirect.com/science/article/pii/S2405844025002786Traditional foodsFlavonoidsAnthocyaninsFunctional foodsObesityDiabetes |
| spellingShingle | Sonia Núñez Adrián Millán-Laleona Javier Cano-Lou Andrea Corella Cristina Moliner Guillermo Cásedas Filippo Maggi Víctor López Carlota Gómez-Rincón Black mulberry (Morus nigra L.) prevents deleterious effects of excess glucose in obese C. elegans decreasing lipofuscin accumulation and ROS production Heliyon Traditional foods Flavonoids Anthocyanins Functional foods Obesity Diabetes |
| title | Black mulberry (Morus nigra L.) prevents deleterious effects of excess glucose in obese C. elegans decreasing lipofuscin accumulation and ROS production |
| title_full | Black mulberry (Morus nigra L.) prevents deleterious effects of excess glucose in obese C. elegans decreasing lipofuscin accumulation and ROS production |
| title_fullStr | Black mulberry (Morus nigra L.) prevents deleterious effects of excess glucose in obese C. elegans decreasing lipofuscin accumulation and ROS production |
| title_full_unstemmed | Black mulberry (Morus nigra L.) prevents deleterious effects of excess glucose in obese C. elegans decreasing lipofuscin accumulation and ROS production |
| title_short | Black mulberry (Morus nigra L.) prevents deleterious effects of excess glucose in obese C. elegans decreasing lipofuscin accumulation and ROS production |
| title_sort | black mulberry morus nigra l prevents deleterious effects of excess glucose in obese c elegans decreasing lipofuscin accumulation and ros production |
| topic | Traditional foods Flavonoids Anthocyanins Functional foods Obesity Diabetes |
| url | http://www.sciencedirect.com/science/article/pii/S2405844025002786 |
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