Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage
A macrophage is an important component of innate immunity which can be activated by infection. A series of inflammatory cytokines are produced and released to eliminate pathogens. CpG DNA is an immune stimulator recognized by TLR9, subsequently inducing inflammatory responses in macrophages. Long no...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2020/1407654 |
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| author | Ying Zheng Xi Luo Zailong Qin Zhiguang Zhou |
| author_facet | Ying Zheng Xi Luo Zailong Qin Zhiguang Zhou |
| author_sort | Ying Zheng |
| collection | DOAJ |
| description | A macrophage is an important component of innate immunity which can be activated by infection. A series of inflammatory cytokines are produced and released to eliminate pathogens. CpG DNA is an immune stimulator recognized by TLR9, subsequently inducing inflammatory responses in macrophages. Long noncoding RNA (lncRNA) is a novel class of noncoding RNA, whose length is more than 200 nt, but without protein-coding capacity. lncRNAs are involved in many physiological and pathological processes, including inflammatory responses. In our study, a lncRNA microarray assay was performed to identify differentially expressed lncRNAs and mRNAs in RAW264.7 cells at different time points following CpG ODN stimulation. The results revealed that expression levels of 734 lncRNAs and 734 mRNAs were altered at all time points. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analyses were performed to predict the functions of dysregulated genes. Coexpression networks of lncRNA-mRNA were constructed based on the correlation analysis between differentially expressed lncRNAs and 10 selected upregulated mRNAs, which have been reported to be involved in CpG DNA-induced inflammatory responses. In addition, we selected 8 dysregulated lncRNAs for further validation by quantitative real-time PCR. The present study provided a systematic perspective on the potential functions of lncRNAs in CpG ODN-induced macrophage activation. |
| format | Article |
| id | doaj-art-d2791df4d3224d27b90bfe617137a68a |
| institution | Kabale University |
| issn | 2314-8861 2314-7156 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-d2791df4d3224d27b90bfe617137a68a2025-02-03T01:27:54ZengWileyJournal of Immunology Research2314-88612314-71562020-01-01202010.1155/2020/14076541407654Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated MacrophageYing Zheng0Xi Luo1Zailong Qin2Zhiguang Zhou3Center for Medical Research, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaDepartment of Metabolism & Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaGenetic and Metabolic Central Laboratory, Maternal and Child Health Hospital of Guangxi, Nanning, Guangxi 530003, ChinaDepartment of Metabolism & Endocrinology, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, ChinaA macrophage is an important component of innate immunity which can be activated by infection. A series of inflammatory cytokines are produced and released to eliminate pathogens. CpG DNA is an immune stimulator recognized by TLR9, subsequently inducing inflammatory responses in macrophages. Long noncoding RNA (lncRNA) is a novel class of noncoding RNA, whose length is more than 200 nt, but without protein-coding capacity. lncRNAs are involved in many physiological and pathological processes, including inflammatory responses. In our study, a lncRNA microarray assay was performed to identify differentially expressed lncRNAs and mRNAs in RAW264.7 cells at different time points following CpG ODN stimulation. The results revealed that expression levels of 734 lncRNAs and 734 mRNAs were altered at all time points. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) biological pathway analyses were performed to predict the functions of dysregulated genes. Coexpression networks of lncRNA-mRNA were constructed based on the correlation analysis between differentially expressed lncRNAs and 10 selected upregulated mRNAs, which have been reported to be involved in CpG DNA-induced inflammatory responses. In addition, we selected 8 dysregulated lncRNAs for further validation by quantitative real-time PCR. The present study provided a systematic perspective on the potential functions of lncRNAs in CpG ODN-induced macrophage activation.http://dx.doi.org/10.1155/2020/1407654 |
| spellingShingle | Ying Zheng Xi Luo Zailong Qin Zhiguang Zhou Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage Journal of Immunology Research |
| title | Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage |
| title_full | Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage |
| title_fullStr | Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage |
| title_full_unstemmed | Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage |
| title_short | Identification of Differentially Expressed lncRNAs in a CpG ODN-Activated Macrophage |
| title_sort | identification of differentially expressed lncrnas in a cpg odn activated macrophage |
| url | http://dx.doi.org/10.1155/2020/1407654 |
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