Molecular characterization and functional annotation of a hypothetical protein (SCO0618) of A3(2)
Streptomyces coelicolor is a gram-positive soil bacterium which is well known for the production of several antibiotics used in various biotechnological applications. But numerous proteins from its genome are considered hypothetical proteins. Therefore, the present study aimed to reveal the function...
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| Format: | Article |
| Language: | English |
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BioMed Central
2020-09-01
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| Series: | Genomics & Informatics |
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| Online Access: | http://genominfo.org/upload/pdf/gi-2020-18-3-e28.pdf |
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| author | Nadim Ferdous Mahjerin Nasrin Reza Md. Tabassum Hossain Emon Md. Shariful Islam A. K. M. Mohiuddin Mohammad Uzzal Hossain |
| author_facet | Nadim Ferdous Mahjerin Nasrin Reza Md. Tabassum Hossain Emon Md. Shariful Islam A. K. M. Mohiuddin Mohammad Uzzal Hossain |
| author_sort | Nadim Ferdous |
| collection | DOAJ |
| description | Streptomyces coelicolor is a gram-positive soil bacterium which is well known for the production of several antibiotics used in various biotechnological applications. But numerous proteins from its genome are considered hypothetical proteins. Therefore, the present study aimed to reveal the functions of a hypothetical protein from the genome of S. coelicolor. Several bioinformatics tools were employed to predict the structure and function of this protein. Sequence similarity was searched through the available bioinformatics databases to find out the homologous protein. The secondary and tertiary structure were predicted and further validated with quality assessment tools. Furthermore, the active site and the interacting proteins were also explored with the utilization of CASTp and STRING server. The hypothetical protein showed the important biological activity having with two functional domain including POD-like_MBL-fold and rhodanese homology domain. The functional annotation exposed that the selected hypothetical protein could show the hydrolase activity. Furthermore, protein-protein interactions of selected hypothetical protein revealed several functional partners those have the significant role for the bacterial survival. At last, the current study depicts that the annotated hypothetical protein is linked with hydrolase activity which might be of great interest to the further research in bacterial genetics. |
| format | Article |
| id | doaj-art-d2784abd36f1489e9054e0781a84a5bb |
| institution | Kabale University |
| issn | 2234-0742 |
| language | English |
| publishDate | 2020-09-01 |
| publisher | BioMed Central |
| record_format | Article |
| series | Genomics & Informatics |
| spelling | doaj-art-d2784abd36f1489e9054e0781a84a5bb2025-02-02T20:47:29ZengBioMed CentralGenomics & Informatics2234-07422020-09-01183e2810.5808/GI.2020.18.3.e28621Molecular characterization and functional annotation of a hypothetical protein (SCO0618) of A3(2)Nadim Ferdous0Mahjerin Nasrin Reza1Md. Tabassum Hossain Emon2Md. Shariful Islam3A. K. M. Mohiuddin4Mohammad Uzzal Hossain5 Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Tangail 1902, Bangladesh Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Tangail 1902, Bangladesh Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Tangail 1902, Bangladesh Laboratory of Reproductive and Developmental Biology, Hokkaido University, Sapporo 060-0808, Japan Department of Biotechnology and Genetic Engineering, Faculty of Life Science, Mawlana Bhashani Science and Technology University, Tangail 1902, Bangladesh Bioinformatics Division, National Institute of Biotechnology, Savar, Dhaka 1349, BangladeshStreptomyces coelicolor is a gram-positive soil bacterium which is well known for the production of several antibiotics used in various biotechnological applications. But numerous proteins from its genome are considered hypothetical proteins. Therefore, the present study aimed to reveal the functions of a hypothetical protein from the genome of S. coelicolor. Several bioinformatics tools were employed to predict the structure and function of this protein. Sequence similarity was searched through the available bioinformatics databases to find out the homologous protein. The secondary and tertiary structure were predicted and further validated with quality assessment tools. Furthermore, the active site and the interacting proteins were also explored with the utilization of CASTp and STRING server. The hypothetical protein showed the important biological activity having with two functional domain including POD-like_MBL-fold and rhodanese homology domain. The functional annotation exposed that the selected hypothetical protein could show the hydrolase activity. Furthermore, protein-protein interactions of selected hypothetical protein revealed several functional partners those have the significant role for the bacterial survival. At last, the current study depicts that the annotated hypothetical protein is linked with hydrolase activity which might be of great interest to the further research in bacterial genetics.http://genominfo.org/upload/pdf/gi-2020-18-3-e28.pdfgenomehypothetical proteinmodelinghydrolases |
| spellingShingle | Nadim Ferdous Mahjerin Nasrin Reza Md. Tabassum Hossain Emon Md. Shariful Islam A. K. M. Mohiuddin Mohammad Uzzal Hossain Molecular characterization and functional annotation of a hypothetical protein (SCO0618) of A3(2) Genomics & Informatics genome hypothetical protein modeling hydrolases |
| title | Molecular characterization and functional annotation of a hypothetical protein (SCO0618) of A3(2) |
| title_full | Molecular characterization and functional annotation of a hypothetical protein (SCO0618) of A3(2) |
| title_fullStr | Molecular characterization and functional annotation of a hypothetical protein (SCO0618) of A3(2) |
| title_full_unstemmed | Molecular characterization and functional annotation of a hypothetical protein (SCO0618) of A3(2) |
| title_short | Molecular characterization and functional annotation of a hypothetical protein (SCO0618) of A3(2) |
| title_sort | molecular characterization and functional annotation of a hypothetical protein sco0618 of a3 2 |
| topic | genome hypothetical protein modeling hydrolases |
| url | http://genominfo.org/upload/pdf/gi-2020-18-3-e28.pdf |
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