Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal
Extended-spectrum β-lactamases (ESBLs) prevalence was studied in the north of Portugal, among 193 clinical isolates belonging to citizens in a district in the boundaries between this country and Spain from a total of 7529 clinical strains. In the present study we recovered some members of Enterobact...
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| Language: | English |
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Wiley
2014-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1155/2014/782897 |
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| author | Rúben Fernandes Paula Amador Carla Oliveira Cristina Prudêncio |
| author_facet | Rúben Fernandes Paula Amador Carla Oliveira Cristina Prudêncio |
| author_sort | Rúben Fernandes |
| collection | DOAJ |
| description | Extended-spectrum β-lactamases (ESBLs) prevalence was studied in the north of Portugal, among 193 clinical isolates belonging to citizens in a district in the boundaries between this country and Spain from a total of 7529 clinical strains. In the present study we recovered some members of Enterobacteriaceae family, producing ESBL enzymes, including Escherichia coli (67.9%), Klebsiella pneumoniae (30.6%), Klebsiella oxytoca (0.5%), Enterobacter aerogenes (0.5%), and Citrobacter freundii (0.5%). β-lactamases genes blaTEM, blaSHV, and blaCTX-M were screened by polymerase chain reaction (PCR) and sequencing approaches. TEM enzymes were among the most prevalent types (40.9%) followed by CTX-M (37.3%) and SHV (23.3%). Among our sample of 193 ESBL-producing strains 99.0% were resistant to the fourth-generation cephalosporin cefepime. Of the 193 isolates 81.3% presented transferable plasmids harboring blaESBL genes. Clonal studies were performed by PCR for the enterobacterial repetitive intragenic consensus (ERIC) sequences. This study reports a high diversity of genetic patterns. Ten clusters were found for E. coli isolates and five clusters for K. pneumoniae strains by means of ERIC analysis. In conclusion, in this country, the most prevalent type is still the TEM-type, but CTX-M is growing rapidly. |
| format | Article |
| id | doaj-art-d272db0873cb4dc5b1c0fd0cac666d27 |
| institution | Kabale University |
| issn | 2356-6140 1537-744X |
| language | English |
| publishDate | 2014-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-d272db0873cb4dc5b1c0fd0cac666d272025-02-03T07:25:40ZengWileyThe Scientific World Journal2356-61401537-744X2014-01-01201410.1155/2014/782897782897Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern PortugalRúben Fernandes0Paula Amador1Carla Oliveira2Cristina Prudêncio3Ciências Químicas e das Biomoléculas (CQB) e Centro de Investigação em Saúde e Ambiente (CISA), Escola Superior de Tecnologia da Saúde do Porto (ESTSP), Instituto Politécnico do Porto (IPP), 4400-330 Porto, PortugalDepartamento de Ciências do Ambiente, Escola Superior Agrária de Coimbra, Instituto Politécnico de Coimbra, Coimbra, PortugalDepartamento de Biologia Molecular, Instituto de Ciência Biomédicas de Abel Salazar (ICBAS), Universidade do Porto, Porto, PortugalCiências Químicas e das Biomoléculas (CQB) e Centro de Investigação em Saúde e Ambiente (CISA), Escola Superior de Tecnologia da Saúde do Porto (ESTSP), Instituto Politécnico do Porto (IPP), 4400-330 Porto, PortugalExtended-spectrum β-lactamases (ESBLs) prevalence was studied in the north of Portugal, among 193 clinical isolates belonging to citizens in a district in the boundaries between this country and Spain from a total of 7529 clinical strains. In the present study we recovered some members of Enterobacteriaceae family, producing ESBL enzymes, including Escherichia coli (67.9%), Klebsiella pneumoniae (30.6%), Klebsiella oxytoca (0.5%), Enterobacter aerogenes (0.5%), and Citrobacter freundii (0.5%). β-lactamases genes blaTEM, blaSHV, and blaCTX-M were screened by polymerase chain reaction (PCR) and sequencing approaches. TEM enzymes were among the most prevalent types (40.9%) followed by CTX-M (37.3%) and SHV (23.3%). Among our sample of 193 ESBL-producing strains 99.0% were resistant to the fourth-generation cephalosporin cefepime. Of the 193 isolates 81.3% presented transferable plasmids harboring blaESBL genes. Clonal studies were performed by PCR for the enterobacterial repetitive intragenic consensus (ERIC) sequences. This study reports a high diversity of genetic patterns. Ten clusters were found for E. coli isolates and five clusters for K. pneumoniae strains by means of ERIC analysis. In conclusion, in this country, the most prevalent type is still the TEM-type, but CTX-M is growing rapidly.http://dx.doi.org/10.1155/2014/782897 |
| spellingShingle | Rúben Fernandes Paula Amador Carla Oliveira Cristina Prudêncio Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal The Scientific World Journal |
| title | Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal |
| title_full | Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal |
| title_fullStr | Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal |
| title_full_unstemmed | Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal |
| title_short | Molecular Characterization of ESBL-Producing Enterobacteriaceae in Northern Portugal |
| title_sort | molecular characterization of esbl producing enterobacteriaceae in northern portugal |
| url | http://dx.doi.org/10.1155/2014/782897 |
| work_keys_str_mv | AT rubenfernandes molecularcharacterizationofesblproducingenterobacteriaceaeinnorthernportugal AT paulaamador molecularcharacterizationofesblproducingenterobacteriaceaeinnorthernportugal AT carlaoliveira molecularcharacterizationofesblproducingenterobacteriaceaeinnorthernportugal AT cristinaprudencio molecularcharacterizationofesblproducingenterobacteriaceaeinnorthernportugal |