Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage
Diabetic retinopathy is the leading cause of blindness, yet its treatment is very limited. Anti-VEGF drug has been widely applied in ocular disease, but its effects on diabetic retinopathy and the underlying mechanism have remained to be fully explored. To elucidate the role of anti-VEGF treatment,...
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| Format: | Article |
| Language: | English |
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Wiley
2018-01-01
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| Series: | Journal of Diabetes Research |
| Online Access: | http://dx.doi.org/10.1155/2018/9216791 |
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| author | Jiao Lv Miao-Miao Chen Zhi-Hao Mu Fang Wang Zhong-Yi Qian Lei Zhou Qiu-Ting Guo Zhi-Min Zhao Yu-Ping Pan Xin-Yu Liao Zhi-Hong Yang Ning Cai Ying-Ying Zou Shu-De Li |
| author_facet | Jiao Lv Miao-Miao Chen Zhi-Hao Mu Fang Wang Zhong-Yi Qian Lei Zhou Qiu-Ting Guo Zhi-Min Zhao Yu-Ping Pan Xin-Yu Liao Zhi-Hong Yang Ning Cai Ying-Ying Zou Shu-De Li |
| author_sort | Jiao Lv |
| collection | DOAJ |
| description | Diabetic retinopathy is the leading cause of blindness, yet its treatment is very limited. Anti-VEGF drug has been widely applied in ocular disease, but its effects on diabetic retinopathy and the underlying mechanism have remained to be fully explored. To elucidate the role of anti-VEGF treatment, we sought to determine the effects of bevacizumab on diabetic neurovascular changes extending from the 3rd to 9th week with induced diabetes in adult rats. The retinal neurovascular changes included increased expression of VEGF, nNOS, iNOS, eNOS, and NO in the course of diabetes progression. In diabetic rats given bevacizumab injection, the ganglion cell loss and alterations of retinal thickness were ameliorated. In this connection, the immunofluorescence labeling of the above biomarkers was noticeably decreased. Along with this, Western blotting confirmed that bevacizumab treatment was associated with a decrease of VEGF, Flk-1, and cAMP response element binding and protein kinase C protein expression. The present results suggest that bevacizumab treatment in the early stage of the retinopathy may ameliorate the lesions of retinopathy, in which VEGF/Flk-1 signaling has been shown here to play an important role. |
| format | Article |
| id | doaj-art-d26b37db681f414da7ee13229899dfb2 |
| institution | Kabale University |
| issn | 2314-6745 2314-6753 |
| language | English |
| publishDate | 2018-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Diabetes Research |
| spelling | doaj-art-d26b37db681f414da7ee13229899dfb22025-02-03T01:24:15ZengWileyJournal of Diabetes Research2314-67452314-67532018-01-01201810.1155/2018/92167919216791Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early StageJiao Lv0Miao-Miao Chen1Zhi-Hao Mu2Fang Wang3Zhong-Yi Qian4Lei Zhou5Qiu-Ting Guo6Zhi-Min Zhao7Yu-Ping Pan8Xin-Yu Liao9Zhi-Hong Yang10Ning Cai11Ying-Ying Zou12Shu-De Li13Department of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, ChinaDepartment of Ophthalmology, First Affiliated Hospital of Kunming Medical University, 295 Xi Chang Road, Kunming 650032, ChinaDepartment of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, ChinaDepartment of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, ChinaDepartment of Morphological Laboratory, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, ChinaThe Key Laboratory of Stem Cell and Regenerative Medicine of Yunnan Province, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, ChinaDepartment of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, ChinaDepartment of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, ChinaDepartment of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, ChinaDepartment of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, ChinaDepartment of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, ChinaDepartment of Ophthalmology, First Affiliated Hospital of Kunming Medical University, 295 Xi Chang Road, Kunming 650032, ChinaDepartment of Pathology and Pathophysiology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, ChinaDepartment of Biochemistry and Molecular Biology, Faculty of Basic Medical Sciences, Kunming Medical University, 1168 West Chunrong Road, Kunming 650500, ChinaDiabetic retinopathy is the leading cause of blindness, yet its treatment is very limited. Anti-VEGF drug has been widely applied in ocular disease, but its effects on diabetic retinopathy and the underlying mechanism have remained to be fully explored. To elucidate the role of anti-VEGF treatment, we sought to determine the effects of bevacizumab on diabetic neurovascular changes extending from the 3rd to 9th week with induced diabetes in adult rats. The retinal neurovascular changes included increased expression of VEGF, nNOS, iNOS, eNOS, and NO in the course of diabetes progression. In diabetic rats given bevacizumab injection, the ganglion cell loss and alterations of retinal thickness were ameliorated. In this connection, the immunofluorescence labeling of the above biomarkers was noticeably decreased. Along with this, Western blotting confirmed that bevacizumab treatment was associated with a decrease of VEGF, Flk-1, and cAMP response element binding and protein kinase C protein expression. The present results suggest that bevacizumab treatment in the early stage of the retinopathy may ameliorate the lesions of retinopathy, in which VEGF/Flk-1 signaling has been shown here to play an important role.http://dx.doi.org/10.1155/2018/9216791 |
| spellingShingle | Jiao Lv Miao-Miao Chen Zhi-Hao Mu Fang Wang Zhong-Yi Qian Lei Zhou Qiu-Ting Guo Zhi-Min Zhao Yu-Ping Pan Xin-Yu Liao Zhi-Hong Yang Ning Cai Ying-Ying Zou Shu-De Li Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage Journal of Diabetes Research |
| title | Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage |
| title_full | Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage |
| title_fullStr | Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage |
| title_full_unstemmed | Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage |
| title_short | Intravitreal Bevacizumab Injection Attenuates Diabetic Retinopathy in Adult Rats with Experimentally Induced Diabetes in the Early Stage |
| title_sort | intravitreal bevacizumab injection attenuates diabetic retinopathy in adult rats with experimentally induced diabetes in the early stage |
| url | http://dx.doi.org/10.1155/2018/9216791 |
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