Site Saturation Mutagenesis Applications on Candida methylica Formate Dehydrogenase
In NADH regeneration, Candida methylica formate dehydrogenase (cmFDH) is a highly significant enzyme in pharmaceutical industry. In this work, site saturation mutagenesis (SSM) which is a combination of both rational design and directed evolution approaches is applied to alter the coenzyme specifici...
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Main Authors: | , , , , , |
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Format: | Article |
Language: | English |
Published: |
Wiley
2016-01-01
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Series: | Scientifica |
Online Access: | http://dx.doi.org/10.1155/2016/4902450 |
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