Site Saturation Mutagenesis Applications on Candida methylica Formate Dehydrogenase

In NADH regeneration, Candida methylica formate dehydrogenase (cmFDH) is a highly significant enzyme in pharmaceutical industry. In this work, site saturation mutagenesis (SSM) which is a combination of both rational design and directed evolution approaches is applied to alter the coenzyme specifici...

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Bibliographic Details
Main Authors: Gülşah P. Özgün, Emel B. Ordu, H. Esra Tütüncü, Emrah Yelboğa, Richard B. Sessions, Nevin Gül Karagüler
Format: Article
Language:English
Published: Wiley 2016-01-01
Series:Scientifica
Online Access:http://dx.doi.org/10.1155/2016/4902450
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