The relationship between histamine receptor type 4 gene polymorphism (rs11662595) and response to H1 antihistamine drugs in patients with chronic idiopathic urticaria
Background and Aim Chronic urticaria is a common allergic skin disease that is characterized by itching and hives, with or without angioedema, that persist for more than 6 weeks. This study aimed to investigate the genetic frequency of the histamine receptor 4 gene polymorphism at SNP rs11662595 in...
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Kurdistan University of Medical Sciences
2024-10-01
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| Series: | مجله علمی دانشگاه علوم پزشکی کردستان |
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| Online Access: | http://sjku.muk.ac.ir/article-1-7621-en.pdf |
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| author | Ebrahim Mohammadi Fakhredin Karimpour Ramin Lotfi Fatemeh Zamani Abbas Aghaei Rasoul Nasiri Kalmarzi |
| author_facet | Ebrahim Mohammadi Fakhredin Karimpour Ramin Lotfi Fatemeh Zamani Abbas Aghaei Rasoul Nasiri Kalmarzi |
| author_sort | Ebrahim Mohammadi |
| collection | DOAJ |
| description | Background and Aim Chronic urticaria is a common allergic skin disease that is characterized by itching and hives, with or without angioedema, that persist for more than 6 weeks. This study aimed to investigate the genetic frequency of the histamine receptor 4 gene polymorphism at SNP rs11662595 in individuals with chronic urticaria and their response to antihistamine treatment and side effects.
Materials and Methods 130 patients with confirmed chronic idiophatic urticaria (CIU) and no other infectious or inflammatory diseases were selected for this study. With the consent of the patients, 5 ml of peripheral blood was taken from each patient for DNA extraction (in a CBC tube) and IgE measurement (in a hemolysis tube without anticoagulant). Genotyping of rs11662595 SNP was determined by PCR-RFLP and total IgE serum level was measured by ELISA method. The disease severity score in the first and twelfth weeks after treatment and the side effects of antihistamine drugs were determined for all patients.
Results: The frequency of rs11662595 polymorphism of HRH4 gene in chronic urticaria patients and healthy control participating in the study was calculated as 17.7% and 18.6%, respectively. However, according to the follow-up during 12 weeks, chronic urticaria patients who had the rs11662595 variant of the HRH4 gene did not show a different response to antihistamine treatment than those without the variant. The only variable that was significantly higher in people with the HRH4 gene variant than in people without this variant was the serum level of D-dimer.
Conclusion: Considering that there was no difference between patients with normal SNP and HRH4 gene polymorphism in any of the examined parameters, it is not important in the response to H1 antihistamine drugs. To find individual genetic responses to antihistamine drugs, other genes involved in the response to these drugs should be examined. |
| format | Article |
| id | doaj-art-d26344326e054ec6801d9ba66932d1bb |
| institution | Kabale University |
| issn | 1560-652X 2345-4040 |
| language | fas |
| publishDate | 2024-10-01 |
| publisher | Kurdistan University of Medical Sciences |
| record_format | Article |
| series | مجله علمی دانشگاه علوم پزشکی کردستان |
| spelling | doaj-art-d26344326e054ec6801d9ba66932d1bb2025-02-01T09:41:06ZfasKurdistan University of Medical Sciencesمجله علمی دانشگاه علوم پزشکی کردستان1560-652X2345-40402024-10-012943948The relationship between histamine receptor type 4 gene polymorphism (rs11662595) and response to H1 antihistamine drugs in patients with chronic idiopathic urticariaEbrahim Mohammadi0Fakhredin Karimpour1Ramin Lotfi2Fatemeh Zamani3Abbas Aghaei4Rasoul Nasiri Kalmarzi5 Kurdistan University of Medical Sciences Kurdistan University of Medical Sciences Kurdistan University of Medical Sciences Kurdistan University of Medical Sciences Kurdistan University of Medical Sciences Kurdistan University of Medical Sciences Background and Aim Chronic urticaria is a common allergic skin disease that is characterized by itching and hives, with or without angioedema, that persist for more than 6 weeks. This study aimed to investigate the genetic frequency of the histamine receptor 4 gene polymorphism at SNP rs11662595 in individuals with chronic urticaria and their response to antihistamine treatment and side effects. Materials and Methods 130 patients with confirmed chronic idiophatic urticaria (CIU) and no other infectious or inflammatory diseases were selected for this study. With the consent of the patients, 5 ml of peripheral blood was taken from each patient for DNA extraction (in a CBC tube) and IgE measurement (in a hemolysis tube without anticoagulant). Genotyping of rs11662595 SNP was determined by PCR-RFLP and total IgE serum level was measured by ELISA method. The disease severity score in the first and twelfth weeks after treatment and the side effects of antihistamine drugs were determined for all patients. Results: The frequency of rs11662595 polymorphism of HRH4 gene in chronic urticaria patients and healthy control participating in the study was calculated as 17.7% and 18.6%, respectively. However, according to the follow-up during 12 weeks, chronic urticaria patients who had the rs11662595 variant of the HRH4 gene did not show a different response to antihistamine treatment than those without the variant. The only variable that was significantly higher in people with the HRH4 gene variant than in people without this variant was the serum level of D-dimer. Conclusion: Considering that there was no difference between patients with normal SNP and HRH4 gene polymorphism in any of the examined parameters, it is not important in the response to H1 antihistamine drugs. To find individual genetic responses to antihistamine drugs, other genes involved in the response to these drugs should be examined.http://sjku.muk.ac.ir/article-1-7621-en.pdfchronic urticariaantihistamine drugshistamine receptorpolymorphism |
| spellingShingle | Ebrahim Mohammadi Fakhredin Karimpour Ramin Lotfi Fatemeh Zamani Abbas Aghaei Rasoul Nasiri Kalmarzi The relationship between histamine receptor type 4 gene polymorphism (rs11662595) and response to H1 antihistamine drugs in patients with chronic idiopathic urticaria مجله علمی دانشگاه علوم پزشکی کردستان chronic urticaria antihistamine drugs histamine receptor polymorphism |
| title | The relationship between histamine receptor type 4 gene polymorphism (rs11662595) and response to H1 antihistamine drugs in patients with chronic idiopathic urticaria |
| title_full | The relationship between histamine receptor type 4 gene polymorphism (rs11662595) and response to H1 antihistamine drugs in patients with chronic idiopathic urticaria |
| title_fullStr | The relationship between histamine receptor type 4 gene polymorphism (rs11662595) and response to H1 antihistamine drugs in patients with chronic idiopathic urticaria |
| title_full_unstemmed | The relationship between histamine receptor type 4 gene polymorphism (rs11662595) and response to H1 antihistamine drugs in patients with chronic idiopathic urticaria |
| title_short | The relationship between histamine receptor type 4 gene polymorphism (rs11662595) and response to H1 antihistamine drugs in patients with chronic idiopathic urticaria |
| title_sort | relationship between histamine receptor type 4 gene polymorphism rs11662595 and response to h1 antihistamine drugs in patients with chronic idiopathic urticaria |
| topic | chronic urticaria antihistamine drugs histamine receptor polymorphism |
| url | http://sjku.muk.ac.ir/article-1-7621-en.pdf |
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