Identification of CβS and ODC antimony resistance markers in anthroponotic cutaneous leishmaniasis field isolates by gene expression profiling
Antiparasitic resistance represents a serious global public health concern with tremendous economic and safety implications. This study intended to investigate the expression of the two major resistant markers: cystathionine β synthase (CβS) and ornithine decarboxylase (ODC) in antimony unresponsive...
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Elsevier
2025-02-01
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| Series: | Parasite Epidemiology and Control |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2405673125000066 |
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| author | Farzaneh Zarrinkar Iraj Sharifi Razieh Tavakoli Oliaee Ali Afgar Elaheh Molaakbari Mehdi Bamorovat Zahra Babaei Ebrahim Eskandari Ehsan Salarkia Marzieh Asadi |
| author_facet | Farzaneh Zarrinkar Iraj Sharifi Razieh Tavakoli Oliaee Ali Afgar Elaheh Molaakbari Mehdi Bamorovat Zahra Babaei Ebrahim Eskandari Ehsan Salarkia Marzieh Asadi |
| author_sort | Farzaneh Zarrinkar |
| collection | DOAJ |
| description | Antiparasitic resistance represents a serious global public health concern with tremendous economic and safety implications. This study intended to investigate the expression of the two major resistant markers: cystathionine β synthase (CβS) and ornithine decarboxylase (ODC) in antimony unresponsive Leishmania tropica isolates compared to responsive ones. Twenty-six patients were randomly selected from widely known foci of anthroponotic cutaneous leishmaniasis in southeastern Iran. Written informed consent of the patients was obtained. Two smears were prepared from the edge of each active lesion; one for microscopic direct smear preparation and the other for inoculation into monophasic NNN media, then for mass production of promastigotes into RPMI-1640 monophasic culture for performing nested PCR and gene expression quantification by real-time PCR. Twenty-six patients consisting of 13 unresponsive and 13 responsive equally distributed among female and male groups. All cases were identified to be L. tropica. Both resistant gene markers were significantly up-regulated in unresponsive and responsive isolates. The findings showed that CβS and ODC are directly linked with the resistance to L. tropica. Alternative drugs or combination therapy and monitoring drug resistance to prevent the spread of resistant isolates are proper strategies to control the disease. |
| format | Article |
| id | doaj-art-d25f05df515043fba1c98aef0ddba471 |
| institution | Kabale University |
| issn | 2405-6731 |
| language | English |
| publishDate | 2025-02-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Parasite Epidemiology and Control |
| spelling | doaj-art-d25f05df515043fba1c98aef0ddba4712025-02-06T05:12:32ZengElsevierParasite Epidemiology and Control2405-67312025-02-0128e00413Identification of CβS and ODC antimony resistance markers in anthroponotic cutaneous leishmaniasis field isolates by gene expression profilingFarzaneh Zarrinkar0Iraj Sharifi1Razieh Tavakoli Oliaee2Ali Afgar3Elaheh Molaakbari4Mehdi Bamorovat5Zahra Babaei6Ebrahim Eskandari7Ehsan Salarkia8Marzieh Asadi9Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, IranLeishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, Iran; Corresponding author.Leishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, IranCenter for Hydatid Disease, Kerman University of Medical Sciences, Kerman, IranLeishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, IranLeishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, IranLeishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, IranCenter for Research and Training in Skin Diseases and Leprosy, Tehran University of Medical Sciences, Tehran, IranLeishmaniasis Research Center, Kerman University of Medical Sciences, Kerman, IranToxoplasmosis Research Center, Department of Parasitology, School of Medicine, Mazandaran University of Medical Sciences, Sari, IranAntiparasitic resistance represents a serious global public health concern with tremendous economic and safety implications. This study intended to investigate the expression of the two major resistant markers: cystathionine β synthase (CβS) and ornithine decarboxylase (ODC) in antimony unresponsive Leishmania tropica isolates compared to responsive ones. Twenty-six patients were randomly selected from widely known foci of anthroponotic cutaneous leishmaniasis in southeastern Iran. Written informed consent of the patients was obtained. Two smears were prepared from the edge of each active lesion; one for microscopic direct smear preparation and the other for inoculation into monophasic NNN media, then for mass production of promastigotes into RPMI-1640 monophasic culture for performing nested PCR and gene expression quantification by real-time PCR. Twenty-six patients consisting of 13 unresponsive and 13 responsive equally distributed among female and male groups. All cases were identified to be L. tropica. Both resistant gene markers were significantly up-regulated in unresponsive and responsive isolates. The findings showed that CβS and ODC are directly linked with the resistance to L. tropica. Alternative drugs or combination therapy and monitoring drug resistance to prevent the spread of resistant isolates are proper strategies to control the disease.http://www.sciencedirect.com/science/article/pii/S2405673125000066Antimonial resistanceLeishmania tropicaGenetic markersCystathionine β synthaseOrnithine decarboxylase |
| spellingShingle | Farzaneh Zarrinkar Iraj Sharifi Razieh Tavakoli Oliaee Ali Afgar Elaheh Molaakbari Mehdi Bamorovat Zahra Babaei Ebrahim Eskandari Ehsan Salarkia Marzieh Asadi Identification of CβS and ODC antimony resistance markers in anthroponotic cutaneous leishmaniasis field isolates by gene expression profiling Parasite Epidemiology and Control Antimonial resistance Leishmania tropica Genetic markers Cystathionine β synthase Ornithine decarboxylase |
| title | Identification of CβS and ODC antimony resistance markers in anthroponotic cutaneous leishmaniasis field isolates by gene expression profiling |
| title_full | Identification of CβS and ODC antimony resistance markers in anthroponotic cutaneous leishmaniasis field isolates by gene expression profiling |
| title_fullStr | Identification of CβS and ODC antimony resistance markers in anthroponotic cutaneous leishmaniasis field isolates by gene expression profiling |
| title_full_unstemmed | Identification of CβS and ODC antimony resistance markers in anthroponotic cutaneous leishmaniasis field isolates by gene expression profiling |
| title_short | Identification of CβS and ODC antimony resistance markers in anthroponotic cutaneous leishmaniasis field isolates by gene expression profiling |
| title_sort | identification of cβs and odc antimony resistance markers in anthroponotic cutaneous leishmaniasis field isolates by gene expression profiling |
| topic | Antimonial resistance Leishmania tropica Genetic markers Cystathionine β synthase Ornithine decarboxylase |
| url | http://www.sciencedirect.com/science/article/pii/S2405673125000066 |
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