p53 Mutations and DNA Ploidy in Colorectal Adenocarcinomas
The p53 tumour suppressor gene has an important role in the the maintenance of genome stability and its mutational inactivation may be at the origin of aneuploidy in cancer cells. The aim of this study was to determine whether p53 mutations were associated to DNA aneuploidy, as assessed by flow cyto...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
1998-01-01
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| Series: | Analytical Cellular Pathology |
| Online Access: | http://dx.doi.org/10.1155/1998/396371 |
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| author | Paola Campomenosi Paola Assereto Massimo Bogliolo Gilberto Fronza Angelo Abbondandolo Antonella Capasso Patrizia F. Bellomo Roberto Monaco Anna Rapallo Andrea Sciutto Roberto Orecchia Elio Geido Walter Giaretti |
| author_facet | Paola Campomenosi Paola Assereto Massimo Bogliolo Gilberto Fronza Angelo Abbondandolo Antonella Capasso Patrizia F. Bellomo Roberto Monaco Anna Rapallo Andrea Sciutto Roberto Orecchia Elio Geido Walter Giaretti |
| author_sort | Paola Campomenosi |
| collection | DOAJ |
| description | The p53 tumour suppressor gene has an important role in the the maintenance of genome stability and its mutational inactivation may be at the origin of aneuploidy in cancer cells. The aim of this study was to determine whether p53 mutations were associated to DNA aneuploidy, as assessed by flow cytometry, in colorectal adenocarcinomas. Analysis of p53 mutations spectrum of the sorted nuclei was done by Denaturing Gradient Gel Electrophoresis (DGGE) and DNA sequencing. Overall, we studied 20 adenocarcinomas, the corresponding control mucosa, and 7 lymph node metastases. |
| format | Article |
| id | doaj-art-d245d921cb234739b76d4daa89b5b012 |
| institution | Kabale University |
| issn | 0921-8912 1878-3651 |
| language | English |
| publishDate | 1998-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Analytical Cellular Pathology |
| spelling | doaj-art-d245d921cb234739b76d4daa89b5b0122025-02-03T01:30:48ZengWileyAnalytical Cellular Pathology0921-89121878-36511998-01-0117111210.1155/1998/396371p53 Mutations and DNA Ploidy in Colorectal AdenocarcinomasPaola Campomenosi0Paola Assereto1Massimo Bogliolo2Gilberto Fronza3Angelo Abbondandolo4Antonella Capasso5Patrizia F. Bellomo6Roberto Monaco7Anna Rapallo8Andrea Sciutto9Roberto Orecchia10Elio Geido11Walter Giaretti12CSTA-Mutagenesis Laboratory, National Cancer Institute-Genova, Genoa, ItalyCSTA-Mutagenesis Laboratory, National Cancer Institute-Genova, Genoa, ItalyCSTA-Mutagenesis Laboratory, National Cancer Institute-Genova, Genoa, ItalyCSTA-Mutagenesis Laboratory, National Cancer Institute-Genova, Genoa, ItalyCSTA-Mutagenesis Laboratory, National Cancer Institute-Genova, Genoa, ItalyCardarelli Hospital, Naples, ItalyCardarelli Hospital, Naples, ItalyCardarelli Hospital, Naples, ItalyBiophysics and Cytometry Laboratory, National Cancer Institute-Genova, Genoa, ItalyBiophysics and Cytometry Laboratory, National Cancer Institute-Genova, Genoa, ItalyBiophysics and Cytometry Laboratory, National Cancer Institute-Genova, Genoa, ItalyBiophysics and Cytometry Laboratory, National Cancer Institute-Genova, Genoa, ItalyBiophysics and Cytometry Laboratory, National Cancer Institute-Genova, Genoa, ItalyThe p53 tumour suppressor gene has an important role in the the maintenance of genome stability and its mutational inactivation may be at the origin of aneuploidy in cancer cells. The aim of this study was to determine whether p53 mutations were associated to DNA aneuploidy, as assessed by flow cytometry, in colorectal adenocarcinomas. Analysis of p53 mutations spectrum of the sorted nuclei was done by Denaturing Gradient Gel Electrophoresis (DGGE) and DNA sequencing. Overall, we studied 20 adenocarcinomas, the corresponding control mucosa, and 7 lymph node metastases.http://dx.doi.org/10.1155/1998/396371 |
| spellingShingle | Paola Campomenosi Paola Assereto Massimo Bogliolo Gilberto Fronza Angelo Abbondandolo Antonella Capasso Patrizia F. Bellomo Roberto Monaco Anna Rapallo Andrea Sciutto Roberto Orecchia Elio Geido Walter Giaretti p53 Mutations and DNA Ploidy in Colorectal Adenocarcinomas Analytical Cellular Pathology |
| title | p53 Mutations and DNA Ploidy in Colorectal Adenocarcinomas |
| title_full | p53 Mutations and DNA Ploidy in Colorectal Adenocarcinomas |
| title_fullStr | p53 Mutations and DNA Ploidy in Colorectal Adenocarcinomas |
| title_full_unstemmed | p53 Mutations and DNA Ploidy in Colorectal Adenocarcinomas |
| title_short | p53 Mutations and DNA Ploidy in Colorectal Adenocarcinomas |
| title_sort | p53 mutations and dna ploidy in colorectal adenocarcinomas |
| url | http://dx.doi.org/10.1155/1998/396371 |
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