Effects of Colchicine and <sup>60</sup>Co-γ Radiation Treatments on the Leaf Size and Fruit Quality of Kiwifruit ‘Donghong’
Colchicine and <sup>60</sup>Co-γ radiation are commonly used breeding techniques for kiwifruit, offering advantages such as low cost, rapid execution, and high efficiency. The buds of red-fleshed kiwifruit (<i>Actinidia chinensis</i>) cv. ‘Donghong’ were used as experimental...
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2025-01-01
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| author | Limei Wang Qing Liu Xishi Li Guanglian Liao Xiaobiao Xu |
| author_facet | Limei Wang Qing Liu Xishi Li Guanglian Liao Xiaobiao Xu |
| author_sort | Limei Wang |
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| description | Colchicine and <sup>60</sup>Co-γ radiation are commonly used breeding techniques for kiwifruit, offering advantages such as low cost, rapid execution, and high efficiency. The buds of red-fleshed kiwifruit (<i>Actinidia chinensis</i>) cv. ‘Donghong’ were used as experimental material and subjected to different concentrations of colchicine and different doses of <sup>60</sup>Co-γ radiation, respectively. Then, the buds were grafted on rootstock, and the ploidy, leaf size, and fruit quality of mutant fruit were evaluated, and principal component analysis (PCA) and simple sequence repeat markers were used to comprehensively assess and detect genetic variations, respectively. The results indicated that a total of 19 buds successfully germinated, with 13 branches successfully bearing fruit. Significant changes were observed in both leaf and fruit morphology following the mutation treatments. Most of the mutant materials showed significant increases in fruit weight, flesh firmness, and soluble sugar content, while titratable acidity and ascorbic acid content significantly decreased. Notably, the 25Gy <sup>60</sup>Co-γ radiation (25d) treatment demonstrated outstanding results, with fruit weight increasing by 256.10%, soluble sugar content rising by 88.29%, titratable acidity decreasing by 29.86%, and ascorbic acid content increasing by 35.60%. PCA results showed that the 25d mutant had the best comprehensive traits. And, except for the 0.4c mutant, all other mutant materials exhibited significant genetic changes at the DNA level. |
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| institution | Kabale University |
| issn | 2311-7524 |
| language | English |
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| spelling | doaj-art-d2452f5d06c942b6aff0a9dbd0931db52025-01-24T13:34:42ZengMDPI AGHorticulturae2311-75242025-01-011117810.3390/horticulturae11010078Effects of Colchicine and <sup>60</sup>Co-γ Radiation Treatments on the Leaf Size and Fruit Quality of Kiwifruit ‘Donghong’Limei Wang0Qing Liu1Xishi Li2Guanglian Liao3Xiaobiao Xu4College of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaCollege of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaCollege of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaCollege of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaCollege of Agronomy, Jiangxi Agricultural University, Nanchang 330045, ChinaColchicine and <sup>60</sup>Co-γ radiation are commonly used breeding techniques for kiwifruit, offering advantages such as low cost, rapid execution, and high efficiency. The buds of red-fleshed kiwifruit (<i>Actinidia chinensis</i>) cv. ‘Donghong’ were used as experimental material and subjected to different concentrations of colchicine and different doses of <sup>60</sup>Co-γ radiation, respectively. Then, the buds were grafted on rootstock, and the ploidy, leaf size, and fruit quality of mutant fruit were evaluated, and principal component analysis (PCA) and simple sequence repeat markers were used to comprehensively assess and detect genetic variations, respectively. The results indicated that a total of 19 buds successfully germinated, with 13 branches successfully bearing fruit. Significant changes were observed in both leaf and fruit morphology following the mutation treatments. Most of the mutant materials showed significant increases in fruit weight, flesh firmness, and soluble sugar content, while titratable acidity and ascorbic acid content significantly decreased. Notably, the 25Gy <sup>60</sup>Co-γ radiation (25d) treatment demonstrated outstanding results, with fruit weight increasing by 256.10%, soluble sugar content rising by 88.29%, titratable acidity decreasing by 29.86%, and ascorbic acid content increasing by 35.60%. PCA results showed that the 25d mutant had the best comprehensive traits. And, except for the 0.4c mutant, all other mutant materials exhibited significant genetic changes at the DNA level.https://www.mdpi.com/2311-7524/11/1/78<sup>60</sup>Co-γ radiationcolchicinefruit qualitykiwifruitsimple sequence repeat |
| spellingShingle | Limei Wang Qing Liu Xishi Li Guanglian Liao Xiaobiao Xu Effects of Colchicine and <sup>60</sup>Co-γ Radiation Treatments on the Leaf Size and Fruit Quality of Kiwifruit ‘Donghong’ Horticulturae <sup>60</sup>Co-γ radiation colchicine fruit quality kiwifruit simple sequence repeat |
| title | Effects of Colchicine and <sup>60</sup>Co-γ Radiation Treatments on the Leaf Size and Fruit Quality of Kiwifruit ‘Donghong’ |
| title_full | Effects of Colchicine and <sup>60</sup>Co-γ Radiation Treatments on the Leaf Size and Fruit Quality of Kiwifruit ‘Donghong’ |
| title_fullStr | Effects of Colchicine and <sup>60</sup>Co-γ Radiation Treatments on the Leaf Size and Fruit Quality of Kiwifruit ‘Donghong’ |
| title_full_unstemmed | Effects of Colchicine and <sup>60</sup>Co-γ Radiation Treatments on the Leaf Size and Fruit Quality of Kiwifruit ‘Donghong’ |
| title_short | Effects of Colchicine and <sup>60</sup>Co-γ Radiation Treatments on the Leaf Size and Fruit Quality of Kiwifruit ‘Donghong’ |
| title_sort | effects of colchicine and sup 60 sup co γ radiation treatments on the leaf size and fruit quality of kiwifruit donghong |
| topic | <sup>60</sup>Co-γ radiation colchicine fruit quality kiwifruit simple sequence repeat |
| url | https://www.mdpi.com/2311-7524/11/1/78 |
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