Sensory Neuropeptides and Endogenous Opioids Expression in Human Dental Pulp with Asymptomatic Inflammation: In Vivo Study
Purpose. This study quantified the expression of substance P (SP), calcitonin gene-related peptide (CGRP), β-endorphins (β-End), and methionine-enkephalin (Met-Enk) in human dental pulp following orthodontic intrusion. Methods. Eight patients were selected according to preestablished inclusion crite...
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| Format: | Article |
| Language: | English |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/879126 |
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| author | Daniel Chavarria-Bolaños Hector Flores-Reyes Nelson Lombana-Sanchez Bernardino Cerda-Cristerna Amaury Pozos-Guillen |
| author_facet | Daniel Chavarria-Bolaños Hector Flores-Reyes Nelson Lombana-Sanchez Bernardino Cerda-Cristerna Amaury Pozos-Guillen |
| author_sort | Daniel Chavarria-Bolaños |
| collection | DOAJ |
| description | Purpose. This study quantified the expression of substance P (SP), calcitonin gene-related peptide (CGRP), β-endorphins (β-End), and methionine-enkephalin (Met-Enk) in human dental pulp following orthodontic intrusion. Methods. Eight patients were selected according to preestablished inclusion criteria. From each patient, two premolars (indicated for extraction due to orthodontic reasons) were randomly assigned to two different groups: the asymptomatic inflammation group (EXPg), which would undergo controlled intrusive force for seven days, and the control group (CTRg), which was used to determine the basal levels of each substance. Once extracted, dental pulp tissue was prepared to determine the expression levels of both neuropeptides and endogenous opioids by radioimmunoassay (RIA). Results. All samples from the CTRg exhibited basal levels of both neuropeptides and endogenous opioids. By day seven, all patients were asymptomatic, even when all orthodontic-intrusive devices were still active. In the EXPg, the SP and CGRP exhibited statistically significant different levels. Although none of the endogenous opioids showed statistically significant differences, they all expressed increasing trends in the EXPg. Conclusions. SP and CGRP were identified in dental pulp after seven days of controlled orthodontic intrusion movement, even in the absence of pain. |
| format | Article |
| id | doaj-art-d22a1341909d46e19391c975e0f1d962 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-d22a1341909d46e19391c975e0f1d9622025-02-03T05:58:49ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/879126879126Sensory Neuropeptides and Endogenous Opioids Expression in Human Dental Pulp with Asymptomatic Inflammation: In Vivo StudyDaniel Chavarria-Bolaños0Hector Flores-Reyes1Nelson Lombana-Sanchez2Bernardino Cerda-Cristerna3Amaury Pozos-Guillen4Faculty of Dentistry, Costa Rica University, 1493-3000 San José, Costa RicaEndodontics Postgraduate Program, Faculty of Dentistry, San Luis Potosi University, 78290 San Luis Potosi, SLP, MexicoResearch and Development, Axopod Consultants for Life, 110921 Bogotá, ColombiaEndodontics Postgraduate Program, Faculty of Dentistry, San Luis Potosi University, 78290 San Luis Potosi, SLP, MexicoEndodontics Postgraduate Program, Faculty of Dentistry, San Luis Potosi University, 78290 San Luis Potosi, SLP, MexicoPurpose. This study quantified the expression of substance P (SP), calcitonin gene-related peptide (CGRP), β-endorphins (β-End), and methionine-enkephalin (Met-Enk) in human dental pulp following orthodontic intrusion. Methods. Eight patients were selected according to preestablished inclusion criteria. From each patient, two premolars (indicated for extraction due to orthodontic reasons) were randomly assigned to two different groups: the asymptomatic inflammation group (EXPg), which would undergo controlled intrusive force for seven days, and the control group (CTRg), which was used to determine the basal levels of each substance. Once extracted, dental pulp tissue was prepared to determine the expression levels of both neuropeptides and endogenous opioids by radioimmunoassay (RIA). Results. All samples from the CTRg exhibited basal levels of both neuropeptides and endogenous opioids. By day seven, all patients were asymptomatic, even when all orthodontic-intrusive devices were still active. In the EXPg, the SP and CGRP exhibited statistically significant different levels. Although none of the endogenous opioids showed statistically significant differences, they all expressed increasing trends in the EXPg. Conclusions. SP and CGRP were identified in dental pulp after seven days of controlled orthodontic intrusion movement, even in the absence of pain.http://dx.doi.org/10.1155/2015/879126 |
| spellingShingle | Daniel Chavarria-Bolaños Hector Flores-Reyes Nelson Lombana-Sanchez Bernardino Cerda-Cristerna Amaury Pozos-Guillen Sensory Neuropeptides and Endogenous Opioids Expression in Human Dental Pulp with Asymptomatic Inflammation: In Vivo Study Mediators of Inflammation |
| title | Sensory Neuropeptides and Endogenous Opioids Expression in Human Dental Pulp with Asymptomatic Inflammation: In Vivo Study |
| title_full | Sensory Neuropeptides and Endogenous Opioids Expression in Human Dental Pulp with Asymptomatic Inflammation: In Vivo Study |
| title_fullStr | Sensory Neuropeptides and Endogenous Opioids Expression in Human Dental Pulp with Asymptomatic Inflammation: In Vivo Study |
| title_full_unstemmed | Sensory Neuropeptides and Endogenous Opioids Expression in Human Dental Pulp with Asymptomatic Inflammation: In Vivo Study |
| title_short | Sensory Neuropeptides and Endogenous Opioids Expression in Human Dental Pulp with Asymptomatic Inflammation: In Vivo Study |
| title_sort | sensory neuropeptides and endogenous opioids expression in human dental pulp with asymptomatic inflammation in vivo study |
| url | http://dx.doi.org/10.1155/2015/879126 |
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