Dysbiosis of gut microbiota with enriched pro-inflammatory species in children with idiopathic short stature: a case-control study

Abstract Idiopathic short state (ISS) demotes a condition of diminished height in children lacking identifiable pathological etiologies, emering as a prominent elements, can precipitate ISS. We explored the prevalence, variability, organization, and contribution of gut microbiota in children affecte...

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Main Authors: Qin Zeng, Xianqiong Feng, Yanling Hu, Shaoyu Su, Bi Ru Luo, Jing Chen
Format: Article
Language:English
Published: Nature Portfolio 2025-06-01
Series:Scientific Reports
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Online Access:https://doi.org/10.1038/s41598-025-04569-1
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author Qin Zeng
Xianqiong Feng
Yanling Hu
Shaoyu Su
Bi Ru Luo
Jing Chen
author_facet Qin Zeng
Xianqiong Feng
Yanling Hu
Shaoyu Su
Bi Ru Luo
Jing Chen
author_sort Qin Zeng
collection DOAJ
description Abstract Idiopathic short state (ISS) demotes a condition of diminished height in children lacking identifiable pathological etiologies, emering as a prominent elements, can precipitate ISS. We explored the prevalence, variability, organization, and contribution of gut microbiota in children affected by ISS. This study selected 58 ISS individuals aged 6–12, serving as the experimental group, 58 non-ISS children constituted the control group. Subsequent to the collection of fresh fecal specimens from both groups, 16 S rRNA gene sequencing facilitated an analysis and juxtaposition of species abundance, species richness, diversity, uniformity, structure, and composition within the intestinal microbiota of the childrens. There were significant differences in the abundance, species richness, diversity, evenness, and colony structure of gut microbiota between the two groups(P < 0.05). Compared with non-ISS children, ISS children have significantly reduced abundance, species richness, diversity, and evenness of their gut microbiota. Upon scrutinizing the gut microbiota composition, In children with ISS, there were 17 increased microorganisms and 13 decreased microorganisms at the three taxonomic levels of families, genus and species.The dominant bacteria in ISS mainly include Peptostreptococcaceae, Prevotella and Porphyromonas bennonis. The abundance, species richness, diversity, and evenness of the gut microbiota decrease, the surge in pro-inflammatory bacteria and key opportunistic pathogens, alongside the reduction of beneficial bacteria and certain opportunistic pathogens, may be linked to the occurrence and development of ISS disease.
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spelling doaj-art-d1f5afbbfbfe49e6b0a2f627f1a7b10d2025-08-20T02:05:38ZengNature PortfolioScientific Reports2045-23222025-06-0115111210.1038/s41598-025-04569-1Dysbiosis of gut microbiota with enriched pro-inflammatory species in children with idiopathic short stature: a case-control studyQin Zeng0Xianqiong Feng1Yanling Hu2Shaoyu Su3Bi Ru Luo4Jing Chen5Department of Pediatrics Nursing, West China Second University Hospital, Sichuan UniversityDepartment of Nursing, West China Hospital, Sichuan University/West China School of Nursing, Nursing Key Laboratory of Sichuan Province, Sichuan UniversityDepartment of Pediatrics Nursing, West China Second University Hospital, Sichuan UniversityDepartment of Pediatrics Nursing, West China Second University Hospital, Sichuan UniversityKey Laboratory of Birth Defects and Related Diseases of Women and Children (Sichuan University), Ministry of EducationDepartment of Pediatrics Nursing, West China Second University Hospital, Sichuan UniversityAbstract Idiopathic short state (ISS) demotes a condition of diminished height in children lacking identifiable pathological etiologies, emering as a prominent elements, can precipitate ISS. We explored the prevalence, variability, organization, and contribution of gut microbiota in children affected by ISS. This study selected 58 ISS individuals aged 6–12, serving as the experimental group, 58 non-ISS children constituted the control group. Subsequent to the collection of fresh fecal specimens from both groups, 16 S rRNA gene sequencing facilitated an analysis and juxtaposition of species abundance, species richness, diversity, uniformity, structure, and composition within the intestinal microbiota of the childrens. There were significant differences in the abundance, species richness, diversity, evenness, and colony structure of gut microbiota between the two groups(P < 0.05). Compared with non-ISS children, ISS children have significantly reduced abundance, species richness, diversity, and evenness of their gut microbiota. Upon scrutinizing the gut microbiota composition, In children with ISS, there were 17 increased microorganisms and 13 decreased microorganisms at the three taxonomic levels of families, genus and species.The dominant bacteria in ISS mainly include Peptostreptococcaceae, Prevotella and Porphyromonas bennonis. The abundance, species richness, diversity, and evenness of the gut microbiota decrease, the surge in pro-inflammatory bacteria and key opportunistic pathogens, alongside the reduction of beneficial bacteria and certain opportunistic pathogens, may be linked to the occurrence and development of ISS disease.https://doi.org/10.1038/s41598-025-04569-1Idiopathic short statureIntestinal microbiotaAssociation analysis16S rRNA gene sequencing
spellingShingle Qin Zeng
Xianqiong Feng
Yanling Hu
Shaoyu Su
Bi Ru Luo
Jing Chen
Dysbiosis of gut microbiota with enriched pro-inflammatory species in children with idiopathic short stature: a case-control study
Scientific Reports
Idiopathic short stature
Intestinal microbiota
Association analysis
16S rRNA gene sequencing
title Dysbiosis of gut microbiota with enriched pro-inflammatory species in children with idiopathic short stature: a case-control study
title_full Dysbiosis of gut microbiota with enriched pro-inflammatory species in children with idiopathic short stature: a case-control study
title_fullStr Dysbiosis of gut microbiota with enriched pro-inflammatory species in children with idiopathic short stature: a case-control study
title_full_unstemmed Dysbiosis of gut microbiota with enriched pro-inflammatory species in children with idiopathic short stature: a case-control study
title_short Dysbiosis of gut microbiota with enriched pro-inflammatory species in children with idiopathic short stature: a case-control study
title_sort dysbiosis of gut microbiota with enriched pro inflammatory species in children with idiopathic short stature a case control study
topic Idiopathic short stature
Intestinal microbiota
Association analysis
16S rRNA gene sequencing
url https://doi.org/10.1038/s41598-025-04569-1
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