Partial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver
Cyanide is one of the most toxic substances present in a wide variety of food materials that are consumed by animals. Rhodanese, a ubiquitous enzyme, can catalyse the detoxification of cyanide by sulphuration reaction. In this study, rhodanese was partially purified and characterized from the liver...
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Wiley
2012-01-01
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| Series: | The Scientific World Journal |
| Online Access: | http://dx.doi.org/10.1100/2012/648085 |
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| author | Hossein Tayefi-Nasrabadi Reza Rahmani |
| author_facet | Hossein Tayefi-Nasrabadi Reza Rahmani |
| author_sort | Hossein Tayefi-Nasrabadi |
| collection | DOAJ |
| description | Cyanide is one of the most toxic substances present in a wide variety of food materials that are consumed by animals. Rhodanese, a ubiquitous enzyme, can catalyse the detoxification of cyanide by sulphuration reaction. In this study, rhodanese was partially purified and characterized from the liver tissue homogenate of the rainbow trout. The enzyme was active in a broad range of pH, from 5 to 12. The optimal activity was found at a high pH (pH 10.5), and the temperature optimum was 25∘C. The enzyme was heat labile, losing > 50% of relative activity after only 5 min of incubation at 40∘C. The Km values for KCN and Na2S2O3 as substrates were 36.81 mM and 19.84 mM, respectively. Studies on the enzyme with a number of cations showed that the activity of the enzyme was not affected by Sn2+, but Hg2+, Ba2+, Pb2+, and Ca2+ inhibited and Cu2+ activated the enzyme with a concentration-dependent manner. |
| format | Article |
| id | doaj-art-d1ed5469d2394e3594f9d6d4e0324e0a |
| institution | Kabale University |
| issn | 1537-744X |
| language | English |
| publishDate | 2012-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | The Scientific World Journal |
| spelling | doaj-art-d1ed5469d2394e3594f9d6d4e0324e0a2025-02-03T05:59:14ZengWileyThe Scientific World Journal1537-744X2012-01-01201210.1100/2012/648085648085Partial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) LiverHossein Tayefi-Nasrabadi0Reza Rahmani1Department of Basic Sciences, College of Veterinary Medicine, University of Tabriz, P.O. Box 51666-16471, Tabriz, IranDepartment of Basic Sciences, College of Veterinary Medicine, University of Tabriz, P.O. Box 51666-16471, Tabriz, IranCyanide is one of the most toxic substances present in a wide variety of food materials that are consumed by animals. Rhodanese, a ubiquitous enzyme, can catalyse the detoxification of cyanide by sulphuration reaction. In this study, rhodanese was partially purified and characterized from the liver tissue homogenate of the rainbow trout. The enzyme was active in a broad range of pH, from 5 to 12. The optimal activity was found at a high pH (pH 10.5), and the temperature optimum was 25∘C. The enzyme was heat labile, losing > 50% of relative activity after only 5 min of incubation at 40∘C. The Km values for KCN and Na2S2O3 as substrates were 36.81 mM and 19.84 mM, respectively. Studies on the enzyme with a number of cations showed that the activity of the enzyme was not affected by Sn2+, but Hg2+, Ba2+, Pb2+, and Ca2+ inhibited and Cu2+ activated the enzyme with a concentration-dependent manner.http://dx.doi.org/10.1100/2012/648085 |
| spellingShingle | Hossein Tayefi-Nasrabadi Reza Rahmani Partial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver The Scientific World Journal |
| title | Partial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver |
| title_full | Partial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver |
| title_fullStr | Partial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver |
| title_full_unstemmed | Partial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver |
| title_short | Partial Purification and Characterization of Rhodanese from Rainbow Trout (Oncorhynchus mykiss) Liver |
| title_sort | partial purification and characterization of rhodanese from rainbow trout oncorhynchus mykiss liver |
| url | http://dx.doi.org/10.1100/2012/648085 |
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