A Sensitive and Selective Method for Determination of Aesculin in Cortex Fraxini by Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Application in Pharmacokinetic Study
A rapid and sensitive method for determining aesculin of Cortex fraxini in rat was developed using high-performance liquid chromatography (HPLC) quadrupole time-of-flight (QTOF) tandem mass (MS/MS). Rat plasma was pretreated by fourfold methanol to remove plasma proteins. Chromatographic separation...
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| Format: | Article |
| Language: | English |
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Wiley
2013-01-01
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| Series: | Journal of Analytical Methods in Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2013/432465 |
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| author | Yi Li Hui Guo Yinying Wu Qianqian Geng Danfeng Dong Huili Wu Enxiao Li |
| author_facet | Yi Li Hui Guo Yinying Wu Qianqian Geng Danfeng Dong Huili Wu Enxiao Li |
| author_sort | Yi Li |
| collection | DOAJ |
| description | A rapid and sensitive method for determining aesculin of Cortex fraxini in rat was developed using high-performance liquid chromatography (HPLC) quadrupole time-of-flight (QTOF) tandem mass (MS/MS). Rat plasma was pretreated by fourfold methanol to remove plasma proteins. Chromatographic separation was performed on a reverse phase column. A tandem mass spectrometric detection with an electrospray ionization (ESI) interface was achieved using collision-induced dissociation (CID) under positive ionization mode. The MS/MS patterns monitored were m/z 341.2716 → m/z 179.1043 for aesculin and m/z 248.3025 → m/z 120.9130 for tinidazole (internal standard). The linear range was calculated to be 10.0–1500.0 ng/mL with a detection limit of 2.0 ng/mL. The inter- and intraday accuracy and precision were within ±7.0%. Pharmacokinetic study showed that aesculin was confirmed to be a one-compartment open model. The method is believed to have good linear range, high sensitivity and recoveries, and superior analytical efficiency. It will probably be an alternative for pharmacokinetic study of aesculin. |
| format | Article |
| id | doaj-art-d1b6c7db67274826b420509d058d3009 |
| institution | Kabale University |
| issn | 2090-8865 2090-8873 |
| language | English |
| publishDate | 2013-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Analytical Methods in Chemistry |
| spelling | doaj-art-d1b6c7db67274826b420509d058d30092025-02-03T01:26:12ZengWileyJournal of Analytical Methods in Chemistry2090-88652090-88732013-01-01201310.1155/2013/432465432465A Sensitive and Selective Method for Determination of Aesculin in Cortex Fraxini by Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Application in Pharmacokinetic StudyYi Li0Hui Guo1Yinying Wu2Qianqian Geng3Danfeng Dong4Huili Wu5Enxiao Li6Department of Medical Oncology, First Affiliated Hospital of Medical College, Xi’an Jiaotong University, No. 277, Yanta Western Road, Xi’an, Shaanxi 710061, ChinaDepartment of Medical Oncology, First Affiliated Hospital of Medical College, Xi’an Jiaotong University, No. 277, Yanta Western Road, Xi’an, Shaanxi 710061, ChinaDepartment of Medical Oncology, First Affiliated Hospital of Medical College, Xi’an Jiaotong University, No. 277, Yanta Western Road, Xi’an, Shaanxi 710061, ChinaDepartment of Medical Oncology, First Affiliated Hospital of Medical College, Xi’an Jiaotong University, No. 277, Yanta Western Road, Xi’an, Shaanxi 710061, ChinaDepartment of Medical Oncology, First Affiliated Hospital of Medical College, Xi’an Jiaotong University, No. 277, Yanta Western Road, Xi’an, Shaanxi 710061, ChinaDepartment of Medical Oncology, First Affiliated Hospital of Medical College, Xi’an Jiaotong University, No. 277, Yanta Western Road, Xi’an, Shaanxi 710061, ChinaDepartment of Medical Oncology, First Affiliated Hospital of Medical College, Xi’an Jiaotong University, No. 277, Yanta Western Road, Xi’an, Shaanxi 710061, ChinaA rapid and sensitive method for determining aesculin of Cortex fraxini in rat was developed using high-performance liquid chromatography (HPLC) quadrupole time-of-flight (QTOF) tandem mass (MS/MS). Rat plasma was pretreated by fourfold methanol to remove plasma proteins. Chromatographic separation was performed on a reverse phase column. A tandem mass spectrometric detection with an electrospray ionization (ESI) interface was achieved using collision-induced dissociation (CID) under positive ionization mode. The MS/MS patterns monitored were m/z 341.2716 → m/z 179.1043 for aesculin and m/z 248.3025 → m/z 120.9130 for tinidazole (internal standard). The linear range was calculated to be 10.0–1500.0 ng/mL with a detection limit of 2.0 ng/mL. The inter- and intraday accuracy and precision were within ±7.0%. Pharmacokinetic study showed that aesculin was confirmed to be a one-compartment open model. The method is believed to have good linear range, high sensitivity and recoveries, and superior analytical efficiency. It will probably be an alternative for pharmacokinetic study of aesculin.http://dx.doi.org/10.1155/2013/432465 |
| spellingShingle | Yi Li Hui Guo Yinying Wu Qianqian Geng Danfeng Dong Huili Wu Enxiao Li A Sensitive and Selective Method for Determination of Aesculin in Cortex Fraxini by Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Application in Pharmacokinetic Study Journal of Analytical Methods in Chemistry |
| title | A Sensitive and Selective Method for Determination of Aesculin in Cortex Fraxini by Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Application in Pharmacokinetic Study |
| title_full | A Sensitive and Selective Method for Determination of Aesculin in Cortex Fraxini by Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Application in Pharmacokinetic Study |
| title_fullStr | A Sensitive and Selective Method for Determination of Aesculin in Cortex Fraxini by Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Application in Pharmacokinetic Study |
| title_full_unstemmed | A Sensitive and Selective Method for Determination of Aesculin in Cortex Fraxini by Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Application in Pharmacokinetic Study |
| title_short | A Sensitive and Selective Method for Determination of Aesculin in Cortex Fraxini by Liquid Chromatography Quadrupole Time-of-Flight Tandem Mass Spectrometry and Application in Pharmacokinetic Study |
| title_sort | sensitive and selective method for determination of aesculin in cortex fraxini by liquid chromatography quadrupole time of flight tandem mass spectrometry and application in pharmacokinetic study |
| url | http://dx.doi.org/10.1155/2013/432465 |
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