In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria
Soft rot disease affects a range of crops in the field and also during transit and storage, resulting in significant yield losses and negative economic impacts. This study evaluated the in vitro antibacterial activities and mode of action of Piper betle extracts against the soft rot disease-causing...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2023-01-01
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| Series: | Scientifica |
| Online Access: | http://dx.doi.org/10.1155/2023/5806841 |
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| _version_ | 1832547391628115968 |
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| author | Punyisa Charirak Rapeepun Prajantasan Kantapon Premprayoon Nikom Srikacha Khakhanang Ratananikom |
| author_facet | Punyisa Charirak Rapeepun Prajantasan Kantapon Premprayoon Nikom Srikacha Khakhanang Ratananikom |
| author_sort | Punyisa Charirak |
| collection | DOAJ |
| description | Soft rot disease affects a range of crops in the field and also during transit and storage, resulting in significant yield losses and negative economic impacts. This study evaluated the in vitro antibacterial activities and mode of action of Piper betle extracts against the soft rot disease-causing bacteria, Erwinia caratovora subsp. caratovora (ECC). Dried leaves of P. betle were extracted with water, ethanol, and hexane solvents and evaluated for their antibacterial activity. The results showed the highest antibacterial activity against ECC in the ethanol extract, followed by hexane and water extracts with minimum inhibitory concentration (MIC) 1.562, 6.25, and more than 12.50 mg/mL, respectively. The time-kill assay indicated a bactericidal mode of action. ECC growth was destroyed within 6 and 8 hours after treatment with the ethanol extract at 4-fold MIC and 2-fold MIC, respectively. The ethanol extract of P. betle showed promising activity against ECC, with the potential for further development as a novel alternative treatment to control phytobacteria. |
| format | Article |
| id | doaj-art-d18863e5c3384c229ec067c21b80c0ba |
| institution | Kabale University |
| issn | 2090-908X |
| language | English |
| publishDate | 2023-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Scientifica |
| spelling | doaj-art-d18863e5c3384c229ec067c21b80c0ba2025-02-03T06:45:08ZengWileyScientifica2090-908X2023-01-01202310.1155/2023/5806841In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing BacteriaPunyisa Charirak0Rapeepun Prajantasan1Kantapon Premprayoon2Nikom Srikacha3Khakhanang Ratananikom4Department of Plant Production TechnologyDepartment of Science and MathematicsDepartment of Agricultural Machinery EngineeringDepartment of Animal ScienceDepartment of Science and MathematicsSoft rot disease affects a range of crops in the field and also during transit and storage, resulting in significant yield losses and negative economic impacts. This study evaluated the in vitro antibacterial activities and mode of action of Piper betle extracts against the soft rot disease-causing bacteria, Erwinia caratovora subsp. caratovora (ECC). Dried leaves of P. betle were extracted with water, ethanol, and hexane solvents and evaluated for their antibacterial activity. The results showed the highest antibacterial activity against ECC in the ethanol extract, followed by hexane and water extracts with minimum inhibitory concentration (MIC) 1.562, 6.25, and more than 12.50 mg/mL, respectively. The time-kill assay indicated a bactericidal mode of action. ECC growth was destroyed within 6 and 8 hours after treatment with the ethanol extract at 4-fold MIC and 2-fold MIC, respectively. The ethanol extract of P. betle showed promising activity against ECC, with the potential for further development as a novel alternative treatment to control phytobacteria.http://dx.doi.org/10.1155/2023/5806841 |
| spellingShingle | Punyisa Charirak Rapeepun Prajantasan Kantapon Premprayoon Nikom Srikacha Khakhanang Ratananikom In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria Scientifica |
| title | In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria |
| title_full | In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria |
| title_fullStr | In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria |
| title_full_unstemmed | In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria |
| title_short | In Vitro Antibacterial Activity and Mode of Action of Piper betle Extracts against Soft Rot Disease-Causing Bacteria |
| title_sort | in vitro antibacterial activity and mode of action of piper betle extracts against soft rot disease causing bacteria |
| url | http://dx.doi.org/10.1155/2023/5806841 |
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