VANGL2 downregulates HINT1 to inhibit the ATM-p53 pathway and promote cisplatin resistance in small cell lung cancer

Abstract Cisplatin is a first-line drug for the treatment of small cell lung cancer (SCLC). Although the majority of patients with SCLC initially respond to cisplatin therapy, cisplatin resistance readily develops, leading to tumor progression. Therefore, this study aims to elucidate the mechanisms...

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Main Authors: Jiayi Xie, Huiying Liu, Chunqian Yang, Weitao Shen, Jian Zhang
Format: Article
Language:English
Published: Nature Publishing Group 2025-04-01
Series:Cell Death Discovery
Online Access:https://doi.org/10.1038/s41420-025-02424-w
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author Jiayi Xie
Huiying Liu
Chunqian Yang
Weitao Shen
Jian Zhang
author_facet Jiayi Xie
Huiying Liu
Chunqian Yang
Weitao Shen
Jian Zhang
author_sort Jiayi Xie
collection DOAJ
description Abstract Cisplatin is a first-line drug for the treatment of small cell lung cancer (SCLC). Although the majority of patients with SCLC initially respond to cisplatin therapy, cisplatin resistance readily develops, leading to tumor progression. Therefore, this study aims to elucidate the mechanisms underlying cisplatin resistance in SCLC. We found that VANGL2 is a poor prognostic factor and promotes cisplatin resistance in SCLC. Mechanistically, in cisplatin-resistant cells, VANGL2 overexpression leads to the autophagic degradation of HINT1. This reduction in HINT1 expression further reduces the phosphorylation of ATM and p53 induced by cisplatin-mediated DNA damage. The decreased phosphorylation of p53 inhibits downstream apoptotic pathways, thereby reducing cisplatin-induced apoptosis. In conclusion, VANGL2 regulates the ATM-p53 pathway-mediated apoptotic response of SCLC to cisplatin by downregulating HINT1, thereby promoting cisplatin resistance. Thus, VANGL2 may serve as a potential therapeutic target for reversing cisplatin resistance in SCLC patients.
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id doaj-art-d17fa8d3e84944b8b51f44c61b0cab0a
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spelling doaj-art-d17fa8d3e84944b8b51f44c61b0cab0a2025-08-20T03:10:09ZengNature Publishing GroupCell Death Discovery2058-77162025-04-0111111210.1038/s41420-025-02424-wVANGL2 downregulates HINT1 to inhibit the ATM-p53 pathway and promote cisplatin resistance in small cell lung cancerJiayi Xie0Huiying Liu1Chunqian Yang2Weitao Shen3Jian Zhang4Department of Oncology, Zhujiang Hospital, Southern Medical UniversityDepartment of Oncology, Zhujiang Hospital, Southern Medical UniversitySchool of Basic Medical Sciences, Chongqing Medical UniversityDepartment of Oncology, Zhujiang Hospital, Southern Medical UniversityDepartment of Oncology, Zhujiang Hospital, Southern Medical UniversityAbstract Cisplatin is a first-line drug for the treatment of small cell lung cancer (SCLC). Although the majority of patients with SCLC initially respond to cisplatin therapy, cisplatin resistance readily develops, leading to tumor progression. Therefore, this study aims to elucidate the mechanisms underlying cisplatin resistance in SCLC. We found that VANGL2 is a poor prognostic factor and promotes cisplatin resistance in SCLC. Mechanistically, in cisplatin-resistant cells, VANGL2 overexpression leads to the autophagic degradation of HINT1. This reduction in HINT1 expression further reduces the phosphorylation of ATM and p53 induced by cisplatin-mediated DNA damage. The decreased phosphorylation of p53 inhibits downstream apoptotic pathways, thereby reducing cisplatin-induced apoptosis. In conclusion, VANGL2 regulates the ATM-p53 pathway-mediated apoptotic response of SCLC to cisplatin by downregulating HINT1, thereby promoting cisplatin resistance. Thus, VANGL2 may serve as a potential therapeutic target for reversing cisplatin resistance in SCLC patients.https://doi.org/10.1038/s41420-025-02424-w
spellingShingle Jiayi Xie
Huiying Liu
Chunqian Yang
Weitao Shen
Jian Zhang
VANGL2 downregulates HINT1 to inhibit the ATM-p53 pathway and promote cisplatin resistance in small cell lung cancer
Cell Death Discovery
title VANGL2 downregulates HINT1 to inhibit the ATM-p53 pathway and promote cisplatin resistance in small cell lung cancer
title_full VANGL2 downregulates HINT1 to inhibit the ATM-p53 pathway and promote cisplatin resistance in small cell lung cancer
title_fullStr VANGL2 downregulates HINT1 to inhibit the ATM-p53 pathway and promote cisplatin resistance in small cell lung cancer
title_full_unstemmed VANGL2 downregulates HINT1 to inhibit the ATM-p53 pathway and promote cisplatin resistance in small cell lung cancer
title_short VANGL2 downregulates HINT1 to inhibit the ATM-p53 pathway and promote cisplatin resistance in small cell lung cancer
title_sort vangl2 downregulates hint1 to inhibit the atm p53 pathway and promote cisplatin resistance in small cell lung cancer
url https://doi.org/10.1038/s41420-025-02424-w
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