Heat Tolerance Differences Between Hu Sheep and Hu Crossbred Sheep in Microbial Community Structure and Metabolism
Background: The frequent occurrence of extreme temperature events causes significant economic losses to the livestock industry. Therefore, delving into the differences in the physiological and molecular mechanisms of heat stress across different sheep breeds is crucial for developing effective manag...
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2025-01-01
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| author | Jing-Da Yuan Li-Wei Wang Shao-Yin Fu Ri-Ge-Li-Tu E Xiao-Qi Ren Hua Sun Fang Liu Biao Wang Jiang-Hong An Meng-Ran Zhao Jiang-Feng He Xiao-Long He |
| author_facet | Jing-Da Yuan Li-Wei Wang Shao-Yin Fu Ri-Ge-Li-Tu E Xiao-Qi Ren Hua Sun Fang Liu Biao Wang Jiang-Hong An Meng-Ran Zhao Jiang-Feng He Xiao-Long He |
| author_sort | Jing-Da Yuan |
| collection | DOAJ |
| description | Background: The frequent occurrence of extreme temperature events causes significant economic losses to the livestock industry. Therefore, delving into the differences in the physiological and molecular mechanisms of heat stress across different sheep breeds is crucial for developing effective management and breeding strategies. Methods: This study explores the differences in heat tolerance mechanisms between Hu sheep and Xinggao sheep by comparing their growth performance under normal and heat stress conditions, as well as examining the differences in physiological, biochemical, and antioxidant indicators related to heat tolerance, serum metabolomics, and gut microbiomics in a heat stress environment. Results: The results indicate that with changes in the temperature–humidity index (THI), Hu sheep exhibit superior stability in respiratory rate (RR) and rectal temperature (RT) fluctuations compared to Xinggao sheep. In terms of biochemical indicators and antioxidant capacity, the levels of creatinine (Cr) and superoxide dismutase (SOD) in Hu sheep serum are significantly higher than those in Xinggao sheep. In comparison, alkaline phosphatase (ALP) and malondialdehyde (MDA) levels are significantly lower. Metabolomic results showed that, compared to Hu sheep, Xinggao sheep exhibited higher cortisol (COR) and dopamine (DA) levels under heat stress conditions, a stronger lipid mobilization capacity, and elevated levels of tricarboxylic acid (TCA) cycle-related metabolites. Furthermore, gut microbiome analysis results indicate that Hu sheep demonstrate stronger cellulose degradation capabilities, as evidenced by significantly higher abundances of microorganisms such as <i>Ruminococcus</i>, <i>Fibrobacter</i>, and <i>Bacteroidales_RF16_group</i>, compared to Xinggao sheep. Conclusions: In summary, Hu sheep exhibit stronger heat tolerance compared to Xinggao sheep. These findings provide an important theoretical basis for the breeding and selection of heat-tolerant meat sheep varieties and offer strong support for the region’s livestock industry in addressing the challenges posed by global warming. |
| format | Article |
| id | doaj-art-d17e66488ebb4687a651cf82436a5936 |
| institution | Kabale University |
| issn | 2218-1989 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Metabolites |
| spelling | doaj-art-d17e66488ebb4687a651cf82436a59362025-01-24T13:41:15ZengMDPI AGMetabolites2218-19892025-01-011514010.3390/metabo15010040Heat Tolerance Differences Between Hu Sheep and Hu Crossbred Sheep in Microbial Community Structure and MetabolismJing-Da Yuan0Li-Wei Wang1Shao-Yin Fu2Ri-Ge-Li-Tu E3Xiao-Qi Ren4Hua Sun5Fang Liu6Biao Wang7Jiang-Hong An8Meng-Ran Zhao9Jiang-Feng He10Xiao-Long He11Inner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, ChinaInner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, ChinaInner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, ChinaInner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, ChinaInner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, ChinaInner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, ChinaInner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, ChinaInner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, ChinaInner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, ChinaInner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, ChinaInner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, ChinaInner Mongolia Academy of Agricultural & Animal Husbandry Sciences, Hohhot 010031, ChinaBackground: The frequent occurrence of extreme temperature events causes significant economic losses to the livestock industry. Therefore, delving into the differences in the physiological and molecular mechanisms of heat stress across different sheep breeds is crucial for developing effective management and breeding strategies. Methods: This study explores the differences in heat tolerance mechanisms between Hu sheep and Xinggao sheep by comparing their growth performance under normal and heat stress conditions, as well as examining the differences in physiological, biochemical, and antioxidant indicators related to heat tolerance, serum metabolomics, and gut microbiomics in a heat stress environment. Results: The results indicate that with changes in the temperature–humidity index (THI), Hu sheep exhibit superior stability in respiratory rate (RR) and rectal temperature (RT) fluctuations compared to Xinggao sheep. In terms of biochemical indicators and antioxidant capacity, the levels of creatinine (Cr) and superoxide dismutase (SOD) in Hu sheep serum are significantly higher than those in Xinggao sheep. In comparison, alkaline phosphatase (ALP) and malondialdehyde (MDA) levels are significantly lower. Metabolomic results showed that, compared to Hu sheep, Xinggao sheep exhibited higher cortisol (COR) and dopamine (DA) levels under heat stress conditions, a stronger lipid mobilization capacity, and elevated levels of tricarboxylic acid (TCA) cycle-related metabolites. Furthermore, gut microbiome analysis results indicate that Hu sheep demonstrate stronger cellulose degradation capabilities, as evidenced by significantly higher abundances of microorganisms such as <i>Ruminococcus</i>, <i>Fibrobacter</i>, and <i>Bacteroidales_RF16_group</i>, compared to Xinggao sheep. Conclusions: In summary, Hu sheep exhibit stronger heat tolerance compared to Xinggao sheep. These findings provide an important theoretical basis for the breeding and selection of heat-tolerant meat sheep varieties and offer strong support for the region’s livestock industry in addressing the challenges posed by global warming.https://www.mdpi.com/2218-1989/15/1/40heat stressmeat sheepthermotolerancemetabolomegut microbiota |
| spellingShingle | Jing-Da Yuan Li-Wei Wang Shao-Yin Fu Ri-Ge-Li-Tu E Xiao-Qi Ren Hua Sun Fang Liu Biao Wang Jiang-Hong An Meng-Ran Zhao Jiang-Feng He Xiao-Long He Heat Tolerance Differences Between Hu Sheep and Hu Crossbred Sheep in Microbial Community Structure and Metabolism Metabolites heat stress meat sheep thermotolerance metabolome gut microbiota |
| title | Heat Tolerance Differences Between Hu Sheep and Hu Crossbred Sheep in Microbial Community Structure and Metabolism |
| title_full | Heat Tolerance Differences Between Hu Sheep and Hu Crossbred Sheep in Microbial Community Structure and Metabolism |
| title_fullStr | Heat Tolerance Differences Between Hu Sheep and Hu Crossbred Sheep in Microbial Community Structure and Metabolism |
| title_full_unstemmed | Heat Tolerance Differences Between Hu Sheep and Hu Crossbred Sheep in Microbial Community Structure and Metabolism |
| title_short | Heat Tolerance Differences Between Hu Sheep and Hu Crossbred Sheep in Microbial Community Structure and Metabolism |
| title_sort | heat tolerance differences between hu sheep and hu crossbred sheep in microbial community structure and metabolism |
| topic | heat stress meat sheep thermotolerance metabolome gut microbiota |
| url | https://www.mdpi.com/2218-1989/15/1/40 |
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