Vascular Endothelial Growth Factor in Tear Samples of Patients with Systemic Sclerosis
Background. Systemic sclerosis is an autoimmune disease, characterized by widespread small vessel vasculopathy, immune dysregulation with production of autoantibodies, and progressive fibrosis. Changes in levels of proangiogenic cytokines had already been determined largely in serum. Our aim was to...
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| Format: | Article |
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Wiley
2015-01-01
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| Series: | Mediators of Inflammation |
| Online Access: | http://dx.doi.org/10.1155/2015/573681 |
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| author | Anikó Rentka Jolán Hársfalvi András Berta Krisztina Köröskényi Zoltán Szekanecz Gabriella Szücs Peter Szodoray Ádám Kemény-Beke |
| author_facet | Anikó Rentka Jolán Hársfalvi András Berta Krisztina Köröskényi Zoltán Szekanecz Gabriella Szücs Peter Szodoray Ádám Kemény-Beke |
| author_sort | Anikó Rentka |
| collection | DOAJ |
| description | Background. Systemic sclerosis is an autoimmune disease, characterized by widespread small vessel vasculopathy, immune dysregulation with production of autoantibodies, and progressive fibrosis. Changes in levels of proangiogenic cytokines had already been determined largely in serum. Our aim was to assess the levels of VEGF in human tears of patients with SSC. Patients and methods. Forty-three patients (40 female and 3 men, mean (SD) age 61 (48–74) years) with SSc and 27 healthy controls were enrolled in this study. Basal tear sample collection and tear velocity investigations were carried out followed by an ophthalmological examination. Total protein concentrations and VEGF levels were determined in tear samples. Results. The average collected tear fluid volume developed 10.4 μL (1.6–31.2) in patients and 15.63 μL (3.68–34.5) in control subjects. The average total protein level was 6.9 μg/μL (1.8–12.3) in tears of patients and control tears contained an average of 4.132 μg/μL (0.1–14.1) protein. In patients with SSc the average concentration of VEGF was 4.9 pg/μL (3.5–8.1) and 6.15 pg/μL (3.84–12.3) in healthy samples. Conclusions. Total protein production was increased because of the smaller tear volume. Decreased VEGF in tear of SSc patients can be explained also by the decreased tear secretion of patients. |
| format | Article |
| id | doaj-art-d1777e9c03634298addc7d85fbb12f36 |
| institution | Kabale University |
| issn | 0962-9351 1466-1861 |
| language | English |
| publishDate | 2015-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Mediators of Inflammation |
| spelling | doaj-art-d1777e9c03634298addc7d85fbb12f362025-02-03T06:42:01ZengWileyMediators of Inflammation0962-93511466-18612015-01-01201510.1155/2015/573681573681Vascular Endothelial Growth Factor in Tear Samples of Patients with Systemic SclerosisAnikó Rentka0Jolán Hársfalvi1András Berta2Krisztina Köröskényi3Zoltán Szekanecz4Gabriella Szücs5Peter Szodoray6Ádám Kemény-Beke7Department of Ophthalmology, University of Debrecen, Nagyerdei Körút 98, Debrecen 4012, HungaryDepartment of Biophysics and Radiation Biology, Semmelweis University, Tűzoltó Utca 37-47, Budapest 1094, HungaryDepartment of Ophthalmology, University of Debrecen, Nagyerdei Körút 98, Debrecen 4012, HungaryInstitute of Biochemistry and Molecular Biology, Signaling and Apoptosis Research Group, Hungarian Academy of Sciences, Research Center of Molecular Medicine, Nagyerdei Körút 98, Debrecen 4012, HungaryDepartment of Rheumatology, Institute of Medicine, Faculty of Medicine, University of Debrecen, Nagyerdei Körút 98, Debrecen 4012, HungaryDepartment of Rheumatology, Institute of Medicine, Faculty of Medicine, University of Debrecen, Nagyerdei Körút 98, Debrecen 4012, HungaryInstitute of Immunology, Rikshospitalet, Oslo University Hospital, Sognsvannsveien 20, 0372 Oslo, NorwayDepartment of Ophthalmology, University of Debrecen, Nagyerdei Körút 98, Debrecen 4012, HungaryBackground. Systemic sclerosis is an autoimmune disease, characterized by widespread small vessel vasculopathy, immune dysregulation with production of autoantibodies, and progressive fibrosis. Changes in levels of proangiogenic cytokines had already been determined largely in serum. Our aim was to assess the levels of VEGF in human tears of patients with SSC. Patients and methods. Forty-three patients (40 female and 3 men, mean (SD) age 61 (48–74) years) with SSc and 27 healthy controls were enrolled in this study. Basal tear sample collection and tear velocity investigations were carried out followed by an ophthalmological examination. Total protein concentrations and VEGF levels were determined in tear samples. Results. The average collected tear fluid volume developed 10.4 μL (1.6–31.2) in patients and 15.63 μL (3.68–34.5) in control subjects. The average total protein level was 6.9 μg/μL (1.8–12.3) in tears of patients and control tears contained an average of 4.132 μg/μL (0.1–14.1) protein. In patients with SSc the average concentration of VEGF was 4.9 pg/μL (3.5–8.1) and 6.15 pg/μL (3.84–12.3) in healthy samples. Conclusions. Total protein production was increased because of the smaller tear volume. Decreased VEGF in tear of SSc patients can be explained also by the decreased tear secretion of patients.http://dx.doi.org/10.1155/2015/573681 |
| spellingShingle | Anikó Rentka Jolán Hársfalvi András Berta Krisztina Köröskényi Zoltán Szekanecz Gabriella Szücs Peter Szodoray Ádám Kemény-Beke Vascular Endothelial Growth Factor in Tear Samples of Patients with Systemic Sclerosis Mediators of Inflammation |
| title | Vascular Endothelial Growth Factor in Tear Samples of Patients
with Systemic Sclerosis |
| title_full | Vascular Endothelial Growth Factor in Tear Samples of Patients
with Systemic Sclerosis |
| title_fullStr | Vascular Endothelial Growth Factor in Tear Samples of Patients
with Systemic Sclerosis |
| title_full_unstemmed | Vascular Endothelial Growth Factor in Tear Samples of Patients
with Systemic Sclerosis |
| title_short | Vascular Endothelial Growth Factor in Tear Samples of Patients
with Systemic Sclerosis |
| title_sort | vascular endothelial growth factor in tear samples of patients with systemic sclerosis |
| url | http://dx.doi.org/10.1155/2015/573681 |
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